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pcDNA3.3-SARS2-B.1.617.2

Plasmid

#172320

Purpose

expressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.

Depositor

David Nemazee

Article

Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

Insert

Spike of B.1.617.2 strain (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19R, 156G, 157-158del, L452R, T478K, D614G, P681…

Promoter

CMV-F

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-FCHO1

Plasmid

#196910

Purpose

visualization of clathrin-coaed vesicle formation in living mammalian cells

Depositor

Kenji Sugimoto

Article

Sakaushi et al Biosci Biotechnol Biochem. 2007 Jul;71(7

Insert

FCHO1 (FCHO1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-CHC17KDP

Plasmid

#59799

Purpose

Expresses knockdown-proof human CHC17 tagged with GFP.

Depositor

Stephen Royle

Article

Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

Insert

CHC17 (CLTC Human)

Tags

GFP

Expression

Mammalian

Mutation

Silent mutations in amino acids 61-65 to confer s…

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223-FER

Plasmid

#23921

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FER (FER Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pT/Caggs-NRASV12

Plasmid

#20205

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

NRas (NRAS Human)

Use

Transposon

Mutation

G12V

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pLCNICK

Plasmid

#84653

Purpose

Genome editing for Lactobacillus casei Lc2W

Depositor

Sheng Yang

Article

Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

Inserts

Cas9 nickase
sgRNA
homology arms of LC2W_2179

Use

CRISPR

Mutation

D10A

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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PT3.5/CMV-EGFRvIII

Plasmid

#20280

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

EGFRvII (EGFR Human)

Use

Transposon

Mutation

See depositor comments below.

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pRevLCav2FeS

Plasmid

#60808

Purpose

to disrupt iron sulfur cluster in yeast Rev3

Depositor

Youri Pavlov, Tahir Tahirov

Article

Baranovskiy et al J Biol Chem. 2012 May 18;287(21):17281-7

Insert

REV3 (REV3 Budding Yeast)

Expression

Yeast

Mutation

contains 647 C-terminal amino acids and 335 bp of…

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only
pSG5-FER

Plasmid

#30191

Depositor

Nora Heisterkamp

Article

Hao et al Mol Cell Biol. 1989 Apr . 9(4):1587-93.

Insert

Fer tyrosine kinase (FER Human)

Expression

Mammalian

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
CCSB-Broad Human Kinase ORF Collection

Plasmid Kit

#1000000014

Purpose

Plasmids for over 500 human kinases and kinase-related proteins in Gateway entry vectors.

Depositor

William Hahn, David Root

Application

Gene Expression and Labeling

Vector Type

Mammalian Expression

Available Since

July 16, 2011

Availability

Academic Institutions and Nonprofits only
His6-PRC1

Plasmid

#69111

Purpose

For bacterial expression of recombinant human PRC1 tagged with His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Protein Regulator of Cytokinesis 1 (PRC1 Human)

Tags

His6

Expression

Bacterial

Available Since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only

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GST-TACC3-His6

Plasmid

#69106

Purpose

For bacterial expression of recombinant human TACC3 tagged with GST and His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Tags

GST and His6

Expression

Bacterial

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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MBP-ch-TOG-His6

Plasmid

#69108

Purpose

For bacterial expression of recombinant human ch-TOG tagged with MBP and His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)

Tags

His6 and MBP

Expression

Bacterial

Available Since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pFBOT563

Plasmid

#40864

Depositor

Harold Gainer

Article

Fields et al PLoS One. 2012;7(2):e32085. Epub 2012 Fe

Insert

Oxytocin promoter

Use

AAV

Expression

Bacterial and Insect

Promoter

mouse oxytocin promoter

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pTBNde(min)

Plasmid

#15125

Depositor

Franco Pagani

Article

Pagani et al Hum Mol Genet. 2003 May 15. 12(10):1111-

Insert

alpha-globin EDB fibronectin minigene (FN1 Human)

Expression

Mammalian

Promoter

alpha-globin

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only

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pGL4.27_ARE/NRF2-SPE

Plasmid

#177775

Purpose

Luciferase reporter for ARE/NRF2

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

NRF2 (NFE2L2 Human)

Use

Luciferase

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-puro_GCaMP6s

Plasmid

#164589

Purpose

Stable integration and expression of GCaMP6s in mammalian cells

Depositor

Joseph Hyser

Article

Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

Insert

GCaMP6s

Use

Lentiviral

Tags

6xHis, T7 tag, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-Ets2

Plasmid

#28128

Depositor

Pumin Zhang

Article

Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

Insert

Ets2 (ETS2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pT2/shp53/GFP4

Plasmid

#20208

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

shp53/GFP4

Use

Transposon

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pHS0810 pcDNA3.1(+) CMV-SV40 NLS-OgeuIscB-nucleoplasmin NLS-3xHA

Plasmid

#176540

Purpose

Mammalian expression of nuclear localized OgeuIscB

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

OgeuIscB

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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