Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 5,103 results for: Ado;

Showing: 31 - 60 of 5103 results

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pcDNA3.1+-Tornado-nLuc

Plasmid

#212611

Purpose

For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

Tornado-CVB3-nLuc

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_Ado1.0med

Plasmid

#153289

Purpose

Express the medium-affinity adenosine sensor GRAB_Ado1.0med in astrocytes

Depositor

Yulong Li

Article

Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Adenosine sensor GRAB_Ado1.0med (medium affinity)

Use

AAV

Promoter

GfaABC1D

Available Since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-Broccoli

Plasmid

#124360

Purpose

Overexpression of circular RNA (Broccoli)

Depositor

Samie Jaffrey

Article

Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

Insert

Tornado-Broccoli

Expression

Mammalian

Promoter

U6+27

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_Ado1.0

Plasmid

#153285

Purpose

Express the adenosine sensor GRAB_Ado1.0 in neurons

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine sensor GRAB_Ado1.0

Use

AAV

Promoter

hSyn

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_Ado1.0

Plasmid

#153288

Purpose

Express the adenosine sensor GRAB_Ado1.0 in astrocytes

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine sensor GRAB_Ado1.0

Use

AAV

Promoter

GfaABC1D

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_Ado1.0mut

Plasmid

#153290

Purpose

Express the adenosine-insensitive control sensor GRAB_Ado1.0mut in astrocytes

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)

Use

AAV

Promoter

GfaABC1D

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_Ado1.0mut

Plasmid

#153287

Purpose

Express the adenosine-insensitive control sensor GRAB_Ado1.0mut in neurons

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)

Use

AAV

Promoter

hSyn

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-RhoBAST

Plasmid

#196341

Purpose

Overexpression of circular RhoBAST

Depositor

Murat Sunbul

Article

Sunbul et al Nat Biotechnol. 2021 Jun;39(6):686-690.

Insert

Tornado-RhoBAST

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-BroccoliSamSensor#1

Plasmid

#124363

Purpose

Overexpression of circular RNA (Broccoli-based SAM sensor)

Depositor

Samie Jaffrey

Article

Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

Insert

Tornado-BroccoliSamSensor#1

Expression

Mammalian

Promoter

U6+27

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_Ado1.0med

Plasmid

#153286

Purpose

Express the medium-affinity adenosine sensor GRAB_Ado1.0med in neurons

Depositor

Yulong Li

Article

Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Has Service

AAV2

Insert

Adenosine sensor GRAB_Ado1.0med (medium affinity)

Use

AAV

Promoter

hSyn

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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miniCMV-tdTomado-tDeg

Plasmid

#185402

Purpose

tdTomado-tDeg fluorogenic protein

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

tdTomado-tDeg

Expression

Mammalian

Mutation

WT

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-Squash

Plasmid

#177915

Purpose

Expresses Squash aptamer as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-Squash

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-biSiRA

Plasmid

#196345

Purpose

Overexpression of circular biSiRA

Depositor

Murat Sunbul

Article

Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

Insert

Tornado-biSiRA

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-ShadowY-kCAAX

Plasmid

#115564

Purpose

dark ITRACK acceptor construct for dual color imaging

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

ShadowY with KRas membrane targeting domain

Tags

ShadowY

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-biRhoBAST

Plasmid

#196344

Purpose

Overexpression of circular biRhoBAST

Depositor

Murat Sunbul

Article

Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

Insert

Tornado-biRhoBAST

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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Pado Red pcDNA3.1

Plasmid

#79665

Purpose

Pado with mKate2

Depositor

Bradley Baker

Article

Kang et al Sci Rep. 2016 Apr 4;6:23865. doi: 10.103

Insert

Pado mKate2

Expression

Mammalian

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pTwist-iS-methadoneSnFR-Golgi

Plasmid

#220865

Purpose

Mammalian cell expression for subcellular pharmacokinetics with Golgi targeting of iS-methadoneSnFR

Depositor

Henry Lester

Article

Muthusamy et al J Am Chem Soc. 2022 May 18;144(19):8480-

Insert

iS-methadoneSnFR

Expression

Mammalian

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-ShadowY-CLC

Plasmid

#193552

Purpose

Expresses mouse Clathrin Light Chain A tagged with mEGFP and ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain A (Clta Mouse)

Tags

ShadowY and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-RhoBAST-MS2

Plasmid

#196342

Purpose

Overexpression of circular RhoBAST-MS2

Depositor

Murat Sunbul

Article

Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

Insert

Tornado-RhoBAST-MS2

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-Tornado-split-nLuc

Plasmid

#212612

Purpose

For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

Tornado-CVB3-split-nLuc

Use

Luciferase

Expression

Mammalian

Mutation

C373T mutation in CVB3

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_Ado1.0

Plasmid

#153291

Purpose

Express the adenosine sensor GRAB_Ado1.0 in Cre positive cells

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine sensor GRAB_Ado1.0

Use

AAV

Promoter

EFS

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-mutTornado-split-nLuc

Plasmid

#212707

Purpose

Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

mutTornado-CVB3-split-nLuc

Mutation

Deleted the partial 3'ribozyme sequence in T…

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-Pepper-RhoBAST

Plasmid

#196343

Purpose

Overexpression of circular Pepper-RhoBAST

Depositor

Murat Sunbul

Article

Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

Insert

Tornado-Pepper-RhoBAST

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Tornado-RNA sensor for MALAT-1

Plasmid

#185406

Purpose

circular RNA-scaffolded sensing modules for MALAT-1

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

circular RNA-scaffolded sensing modules

Expression

Mammalian

Mutation

WT

Promoter

U6

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_Ado1.0med

Plasmid

#153292

Purpose

Express the medium-affinity adenosine sensor GRAB_Ado1.0med in Cre positive cells

Depositor

Yulong Li

Article

Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Adenosine sensor GRAB_Ado1.0med (medium affinity)

Use

AAV

Promoter

EFS

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV Aquamarine-shadowG tandem

Plasmid

#157774

Purpose

Produces a fusion between Aquamarine and shadowG. It can be used as a positive control for FRET (using FLIM)

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Type

Empty backbone

Tags

Aquamarine and shadowG

Expression

Mammalian

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV ShadowG-AURKA-mTurq2

Plasmid

#157768

Purpose

Expression of AuroraA kinase biosensor under CMV promoter

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

mTurquoise2 and shadowG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV Aquamarine-shadowY tandem

Plasmid

#157775

Purpose

Produces a fusion between Aquamarine and shadowY. It can be used as a positive control for FRET (using FLIM)

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Type

Empty backbone

Tags

Aquamarine and shadowY

Expression

Mammalian

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Tornado-RNA sensor for survivin

Plasmid

#185405

Purpose

circular RNA-scaffolded sensing modules for survivin

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

circular RNA-scaffolded sensing modules

Expression

Mammalian

Mutation

WT

Promoter

U6

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV ShadowY-AURKA-mTurq2

Plasmid

#157770

Purpose

Expression of AuroraA kinase biosensor under CMV promoter

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

mTurquoise2 and shadowY

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 31 - 60 of 5103 results

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