We narrowed to 6,369 results for: itch

1. pTcI39-switch

   Plasmid

   #195557

   Purpose

   Temperature state switch expressing low-temperature mWasabi and high-temperature mRFP1

   Depositor

   Mikhail Shapiro

   Article

   Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

   Inserts

   mRFP1
   mWasabi
   TcI39
   

   Use

   Tags

   6x His and AANDENYADAS degradation tag

   Expression

   Bacterial

   Mutation

   A67T

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Twitch-2B pRSETB

   Plasmid

   #48203

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch-2B
   

   Use

   Tags

   6xHIS, cpVenusCD, and mCerulean3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. Twitch-2B pcDNA3

   Plasmid

   #49531

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch-2B
   

   Use

   Tags

   ECFP and cpCit174

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pN-PITCh-GFP

   Plasmid

   #127888

   Purpose

   Provides the repair template with PSMD2 microhomology on each end for PSMD2 N-ter GFP tagging

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Insert

   Puro-GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Twitch-1 pcDNA3

   Plasmid

   #49812

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch-1
   

   Use

   Tags

   ECFP and cpCit174

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pN-PITCh-3XHA

   Plasmid

   #127881

   Purpose

   Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HA tagging

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Insert

   GFP-Puro-3XHA (PPP2CA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Twitch-4 pcDNA3

   Plasmid

   #49533

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch-4
   

   Use

   Tags

   ECFP and cpCit174

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

8. Twitch-1 pRSETB

   Plasmid

   #48207

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Mues et al Nat Med. 2013 Jun;19(6):778-83. doi: 10.

   Insert

   Twitch-1
   

   Use

   Tags

   6xHIS, ECFP, and cpCit174

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

9. Twitch-3 pcDNA3

   Plasmid

   #49532

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch-3
   

   Use

   Tags

   ECFP and cpCit174

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pN-PITCh-mCherry

    Plasmid

    #127887

    Purpose

    Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter mCherry tagging

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Insert

    Puro-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.hSyn1.Twitch2B.WPRE.SV40

    Plasmid

    #100040

    Purpose

    AAV expression of Twitch2B driven from hSyn1 promoter. Twitch 2B is a fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Has Service

    AAV1

    Insert

    Twitch2B
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pN-PITCh-HBTH

    Plasmid

    #127883

    Purpose

    Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HBTH tagging

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Insert

    GFP-Puro-HBTH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pN-PITCh-FAID

    Plasmid

    #127885

    Purpose

    Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter FAID tagging

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Insert

    GFP-Puro-H3F-AID (PPP2CA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pN-PITCh-H3FAID

    Plasmid

    #127884

    Purpose

    Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3FAID tagging

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Insert

    GFP-Puro-H3F-AID
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Twitch-2C pcDNA3

    Plasmid

    #104501

    Purpose

    fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-2C
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC-STITCH

    Plasmid

    #129535

    Purpose

    STITCH (Synthetic Toplogical Insulator with TetO for CHromatin-engineering) with homology arms for MYC locus insertion.

    Depositor

    Hideyuki Okano

    Article

    Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

    Insert

    STITCH
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Twitch-3 pRSETB

    Plasmid

    #48204

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-3A
    

    Use

    Tags

    6xHIS, cpVenusCD, and mCerulean3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pBS_KS_attB2_SA(0)_T2AGeneSwitch

    Plasmid

    #125214

    Purpose

    Artificial phase 0 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GeneSwitch
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. p204-U6-Switch-ON

    Plasmid

    #217883

    Purpose

    Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAG_Ptet*_ToeRep_N01_switch

    Plasmid

    #132737

    Purpose

    Inducible expression of first-generation toehold repressor index 1 switch RNA with a GFP-ASV reporter via the Ptet* promoter

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    Ptet*_ToeRep_N01_switch
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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