We narrowed to 6,924 results for: pcr

1. pCRISPRyl_MFE1

   Plasmid

   #84612

   Purpose

   Introduces DSB at MFE1 locus in Y. lipolytica

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   UAS1B8-TEF(136)

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPRyl_D17

   Plasmid

   #84611

   Purpose

   Introduces DSB at D17 locus in Y. lipolytica

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   UAS1B8-TEF(136)

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPRyl_A08

   Plasmid

   #84610

   Purpose

   Introduces DSB at A08 locus in Y. lipolytica

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   UAS1B8-TEF(136)

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPRyl_XPR2

   Plasmid

   #84609

   Purpose

   Introduces DSB at XPR2 locus in Y. lipolytica

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   UAS1B8-TEF(136)

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPomyces-2

   Plasmid

   #61737

   Purpose

   Streptomyces expression of codon-optimized Cas9 and custom gRNA

   Depositor

   Huimin Zhao

   Article

   Cobb et al ACS Synth Biol. 2014 Dec 8.

   Inserts

   sSpCas9
   gRNA cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   BbsI-flanked lacZ cassette inserted in place of s…

   Promoter

   gapdhp(EL) and rpsL(XC)-BbsI

   Available since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCR1265

   Plasmid

   #111094

   Purpose

   lentiviral cDNA expression vector: plenti-x1-neo-linker-IRES-Thy1.1

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRZeroT

   Plasmid

   #120276

   Purpose

   Bifunctional vector for TA-cloning and blunt-end cloning of PCR-products using ZERO-backgound system

   Depositor

   Ken Motohashi

   Article

   Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCrlM

   Plasmid

   #122444

   Purpose

   Expresses myc tagged mouse reelin ORF in mammalian cells

   Depositor

   Tom Curran

   Article

   D'Arcangelo et al J Neurosci. 1997 Jan 1;17(1):23-31.

   Insert

   reelin (Reln Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET22B_SA_PcrA

   Plasmid

   #102999

   Purpose

   The plasmid is used to express Staphylococcus aureus PcrA, a DNA helicase, with N-terminal His-tag and Gly-Gly linker

   Depositor

   Martin Webb

   Insert

   SA_PcrA
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   PcrA 199-2423

   Promoter

   T7

   Available since

   May 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRZero

    Plasmid

    #120275

    Purpose

    Blunt-end cloning vector of PCR-products using ZERO-backgound system

    Depositor

    Ken Motohashi

    Article

    Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCrl

    Plasmid

    #122443

    Purpose

    Expresses entire mouse reelin ORF in mammalian cells

    Depositor

    Tom Curran

    Article

    D'Arcangelo et al J Neurosci. 1997 Jan 1;17(1):23-31.

    Insert

    reelin (Reln Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRII_TOPO_hNEAT1

    Plasmid

    #61518

    Purpose

    human NEAT1_v1 short isoform 3.7kb insert in general cloning vector

    Depositor

    Archa Fox

    Article

    Clemson et al Mol Cell. 2009 Mar 27;33(6):717-26. doi:

    Insert

    NEAT1 (NEAT1 Human)
    

    Use

    General cloning vector

    Tags

    Expression

    Mutation

    *see comment below

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid

    #91249

    Purpose

    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPathBrick

    Plasmid

    #65006

    Purpose

    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive native promoters

    Available since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCR1307

    Plasmid

    #111133

    Purpose

    Expresses WDR48.

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    WDR48 (WDR48 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCRT

    Plasmid

    #120274

    Purpose

    TA-cloning vector of PCR-products

    Depositor

    Ken Motohashi

    Article

    Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCR1325

    Plasmid

    #111132

    Purpose

    Expresses USP1.

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    USP1 (USP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCR1068

    Plasmid

    #111092

    Purpose

    BFP/puromycin marked gRNA vector. Contains sgRNA against GFP/BFP N-terminus.

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    BlpI site disrupted

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPRi

    Plasmid

    #199808

    Purpose

    vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

    Depositor

    Steve Salipante

    Article

    Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

    Inserts

    dCas9
    sgRNA with GFP cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCri-1b

    Plasmid

    #61327

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag and MBP-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only