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Plasmid#109934PurposeProtein expression and purification of PPP1CA_MetallophosDepositorInsertPPP1CA_Metallophos
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHO_pTEFmut7_CggR_R250A_ble
Plasmid#124585PurposePlasmid for genomic integration of CggR_R250A repressor regulated by the pTEFmut7 promoterDepositorInsertcggR_R250A (cggR Synthetic)
UseSynthetic BiologyTagsExpressionMutationchanged arginine 250 to alaninePromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV2_hSyn_Phobos_CA_Citrine
Plasmid#98218PurposeSlow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.DepositorInsertSynthetic construct Phobos_C128A gene
UseAAVTagsCitrineExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…Promoterhuman synapsinAvailable sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV2_hSyn_Phobos_Citrine
Plasmid#98216PurposeBlue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.DepositorInsertSynthetic construct Phobos gene
UseAAVTagsCitrineExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…Promoterhuman synapsinAvailable sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHO_pTEFmut7_CggR_ble
Plasmid#124584PurposePlasmid for genomic integration of CggR repressor regulated by the pTEFmut7 promoterDepositorInsertcggR (cggR Synthetic)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Stenotrophomonas_maltophilia
Plasmid#224396PurposeFor expression of the type I CBASS system encoded by Stenotrophomonas maltophiliaDepositorInsertCap4_SmCdnD
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHOHO2_Hsp82viiA
Plasmid#61708PurposeYeast integration plasmid targeted to the HO loci with heterodimeric Hsp82. Digest with NotI prior to addition to yeast.DepositorInsertHsp82viiA (HSP82 Budding Yeast)
UseYeast integration vector targeting inserts to the…Tags6xHisExpressionYeastMutationHeteromeric coiled coil inserted after amino acid…PromoterTEF1Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-AmphoEnv
Plasmid#15799DepositorInsert4070A Amphotropic Murine leukemia virus envelope gene
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
Lambda Phosphatase
Plasmid#79748PurposeIntended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.DepositorInsertLambda Phosphatase
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
phOct4-EGFP
Plasmid#38776DepositorInsertOct-4 promoter (POU5F1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterhuman OCT4Available sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHO4d-Cas9
Plasmid#67881PurposeCas9nlsHis6 Bacterial Expression constructDepositorInsertCas9
UseCRISPRTagsHis6 and T7 epitopeExpressionBacterialMutationPromoterT7Available sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PTPN22_Y-phosphatase
Plasmid#110048PurposeProtein expression and purification of PTPN22_Y-phosphataseDepositorInsertPTPN22_Y-phosphatase (PTPN22 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBad-PhoCl2c
Plasmid#164035PurposeImproved PhoCl variant with enhanced dissociation efficiencyDepositorInsertPhoCl2c
UseTagsHis tag, T7 tag, Xpress TagExpressionBacterialMutationPromoteraraBad promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBad-PhoCl2f
Plasmid#164036PurposeImproved PhoCl variant with faster dissociation rateDepositorInsertPhoCl2f
UseTagsHis tag, T7 tag, Xpress TagExpressionBacterialMutationPromoteraraBad promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
phOCT4-Luc
Plasmid#17221DepositorInsertUpstream region of human OCT4 (POU5F1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PhoDer
Plasmid#188473PurposeExpresses the optogenetic human Gasdermin D (PhoDer) in mammalian cellsDepositorInsertPhoDer (GSDMD Human, Synthetic)
UseTagsExpressionMammalianMutationPromoterCMV IEAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHOT-P2Y2R
Plasmid#159108Purposeentiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high affinity G-protein coupled receptor that mobilizes Ca2+ from intracellular stores upon binding extracellular ATP.48,49 By using the GibsonDepositorInsertP2Y2R
UseLentiviralTagsExpressionMutationPromoterUBCAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
TfR-pHoran4
Plasmid#61547PurposeHuman transferrin receptor tagged with pH sensitive orange/red fluorescent protein pHoran4DepositorInsertTransferrin receptor (TFRC Human)
UseTagspHoran4ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMC059-HisMS2_PLP_NucPhos_pac
Plasmid#155039PurposeGeneration of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Nucleocapsid Phosphoprotein geneDepositorInsertsMaturation Protein
Coat Protein Dimer
Nucleocapsid Phosphoprotein
UseTagsExpressionBacterialMutationRemove TypeIIs Restriction SitesPromoterT7Available sinceSept. 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDisplay-pHoran4
Plasmid#61557PurposeOrange fluorescent protein based pH indicator in pDisplay vectorDepositorInsertpHoran4
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only