Addgene

We narrowed to 9,334 results for: FIE

Showing: 381 - 400 of 9334 results

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pFUGW hUbC-Sub-dCas9-KRAB-T2A-GFP

Plasmid

#227012

Purpose

Lentiviral Expression of dCas9-KRAB from S. uberis fused to the repressive KRAB domain

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Expression

Mutation

Promoter

UbC

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pEThT-MAHS delta MTP

Plasmid

#90032

Purpose

To express His-tagged MAHS in bacteria

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

MAHS

Use

Tags

His

Expression

Bacterial

Mutation

Deletion of mitochondria targeting peptide

Promoter

T7

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-Axin1

Plasmid

#109370

Purpose

Transient over-expression of human Axin1

Depositor

Mariann Bienz

Article

Fiedler et al Proc Natl Acad Sci U S A. 2011 Feb 1;108

Insert

Axin1 (AXIN1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pDB1 (CK2alpha)

Plasmid

#27083

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha (CSNK2A1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pAB46 (CK2beta)

Plasmid

#27085

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2beta (CSNK2B Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 17, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV.minBG-EGFP-W3SL_2x-U6-sasgAi14

Plasmid

#231365

Purpose

minBG-driven EGFP, also encoding U6-driven sgRNAs to direct saCas9 to both sides of stop cassette in Ai14 mice.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.Ple155-CI-EGFP-W3SL_2x-U6-sasgAi14

Plasmid

#231366

Purpose

Ple155-driven EGFP, also encoding U6-driven sgRNAs to direct saCas9 to both sides of stop cassette in Ai14 mice.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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HA-human-BNIP3

Plasmid

#100781

Purpose

Mammalian expression of BNIP3

Depositor

Joseph Gordon

Article

Field et al Cell Death Discov. 2018 Sep 21;4:37. doi

Insert

human-BNIP3 (BNIP3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pSPIH6

Plasmid

#190676

Purpose

E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)

Depositor

John Vederas

Article

Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi

Inserts

Small ubiquitin-like modifier
Mxe GyrA intein with C-terminal chitin binding domain and His tag

Use

Tags

Chitin binding domain, His tag, and N-terminal Hi…

Expression

Bacterial

Mutation

Added C-terminal his tag and None

Promoter

T7

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pZW12 (CK2beta)

Plasmid

#27088

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2beta (CSNK2B Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pDB6 (CK2alpha')

Plasmid

#27084

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha' (CSNK2A2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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F-MED26

Plasmid

#15367

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

Insert

MED26 (MED26 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pET28a-pro-sumo1

Plasmid

#25101

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

pro-SUMO1 (SUMO1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Changed Serine 2 to Alanine

Promoter

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only
pGV15 (CK2alpha K68M)

Plasmid

#27089

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha K68M (kinase dead) (CSNK2A1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K68M (kinase dead)

Promoter

CMV

Available since

May 17, 2012

Availability

Academic Institutions and Nonprofits only
pET21a-TEX264-deltaLRR-His6

Plasmid

#220215

Purpose

Bacterial expression of TEX264, 2-25 residues (LRR) truncated, C-terminal His6 tag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Use

Tags

His6

Expression

Bacterial

Mutation

c.4-75del / p.2-24del

Promoter

T7

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5 TEX264wt-cSSH

Plasmid

#220209

Purpose

Mammalian expression of wild type TEX264, C-terminal twin-Strep_HA tag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Use

Tags

Strep-strep-HA

Expression

Mammalian

Mutation

Promoter

CMV/TetO

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pZW16 (CK2alpha')

Plasmid

#27087

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha' (CSNK2A2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2012

Availability

Academic Institutions and Nonprofits only

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dmBACCS2NS-IRES-dOrai-IRES-GFP

Plasmid

#72895

Purpose

Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2NS-IRES-dOrai-IRES-GFP

Use

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pGP18 (CK2alpha' K69M)

Plasmid

#27090

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha' K69M (CSNK2A2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K69M

Promoter

CMV

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pAB07 (CK2beta)

Plasmid

#27091

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2beta (CSNK2B Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2012

Availability

Academic Institutions and Nonprofits only