We narrowed to 1,604 results for: N-pac

1. pHLS-EF1a-FKBP12-Gag(HIV)

   Plasmid

   #138476

   Purpose

   Expresses FKBP12 fused Gag for packaging FRB-SpCas9 into NanoMEDIC particle.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   HIV Gag
   

   Tags

   FRBP12 and Lynn Myristolation site

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PL-5LTR-RGR(DMD​#​1​)-AmCyan-A

   Plasmid

   #138482

   Purpose

   Expresses sgRNA targeting human DMD (Dystrophin) for packaging into NanoMEDIC particle.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   Ribozyme-flanked gRNA and AmCyan
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-hSyn-ZipT-mScarlet-KV2.1

   Plasmid

   #221617

   Purpose

   A recombinant AAV2 plasmid encoding the ZipACR I151V mutant and soma targeting with KV2.1 motif.

   Depositor

   John Lin

   Article

   Mermet-Joret et al eLife 2023 12:RP90327

   Insert

   ZipACR (I151T)-mScarlet with soma targeting
   

   Use

   AAV

   Mutation

   ZipACR (I151T) with soma targeting

   Promoter

   Human Synapsin

   Available Since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-hSyn-ZipV-mScarlet-KV2.1

   Plasmid

   #221616

   Purpose

   A recombinant AAV2 plasmid encoding the ZipACR I151T mutant and soma targeting with KV2.1 motif.

   Depositor

   John Lin

   Article

   Mermet-Joret et al eLife 2023 12:RP90327

   Insert

   ZipACR (I151V)-mScarlet with soma targeting
   

   Use

   AAV

   Mutation

   ZipACR (I151V) with soma targeting

   Promoter

   Human Synapsin

   Available Since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHLS-EF1a-FRB-SpCas9-A

   Plasmid

   #138477

   Purpose

   Expresses FRB fused SpCas9 for NanoMEDIC packaging.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   SpCas9, human codon optimized
   

   Use

   CRISPR

   Tags

   3x HA tag and FRB

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pINS-Puro

   Plasmid

   #31389

   Depositor

   Yegor Vassetzky

   Article

   Dmitriev et al BMC Res Notes. 2008 Dec 23;1:135. doi: 1

   Insert

   pac
   

   Use

   Cre/Lox

   Available Since

   Sept. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTE5400_pMBP_CDS dropout vector

   Plasmid

   #221189

   Purpose

   Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

   Depositor

   Tobias Erb

   Article

   Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

   Type

   Empty backbone

   Tags

   10x His-tag + E. coli maltose binding protein (MB…

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-hSyn-ZipT-2A-IvfChr (citrine, KV2.1)

   Plasmid

   #221619

   Purpose

   A recombinant AAV2 plasmid encoding the ZipACR I151T mutant with soma targeting with KV2.1 motif and membrane expression enhanced vfChrimson-Citrine with soma targeting KV2.1 motif.

   Depositor

   John Lin

   Article

   Mermet-Joret et al eLife 2023 12:RP90327

   Insert

   ZipACR (I151V)KV2.1-2A-IvfChr-citrine-Kv2.1
   

   Use

   AAV

   Mutation

   ZipACR (I151T) with soma targeting, vfChrimson w…

   Promoter

   Human Synapsin

   Available Since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-hSyn-ZipT-2A-IvfChr (mCherry, KV2.1)

   Plasmid

   #221621

   Purpose

   A recombinant AAV2 plasmid encoding the ZipACR I151T mutant with soma targeting with KV2.1 motif and membrane expression enhanced vfChrimson-mCHerry with soma targeting KV2.1 motif.

   Depositor

   John Lin

   Article

   Mermet-Joret et al eLife 2023 12:RP90327

   Insert

   ZipACR (I151V)KV2.1-2A-IvfChr-mCherry-Kv2.1
   

   Use

   AAV

   Mutation

   ZipACR (I151T) with soma targeting, vfChrimson w…

   Promoter

   Human Synapsin

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-hSyn-ZipV-2A-IvfChr (citrine, KV2.1)

    Plasmid

    #221618

    Purpose

    A recombinant AAV2 plasmid encoding the ZipACR I151V mutant with soma targeting with KV2.1 motif and membrane expression enhanced vfChrimson-Citrine with soma targeting KV2.1 motif.

    Depositor

    John Lin

    Article

    Mermet-Joret et al eLife 2023 12:RP90327

    Insert

    ZipACR (I151V)KV2.1-2A-IvfChr-citrine-Kv2.1
    

    Use

    AAV

    Mutation

    ZipACR (I151V) with soma targeting, vfChrimson w…

    Promoter

    Human Synapsin

    Available Since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-FRT-TO-FH-Nprot

    Plasmid

    #157729

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nprot (N SARS-CoV-2)
    

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Available Since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pJT329 Lenti pEF-rTetR(SE-G72P)-3XFLAG-N+Z+F-T2A-mCherry-BSD-WPRE

    Plasmid

    #187339

    Purpose

    rTetR effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    rTetR(SE-G72P)-3XFLAG-N+Z+F-T2A-mCherry-BSD-WPRE
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-SpRYc-ABE8e-EGFP

    Plasmid

    #208340

    Purpose

    Expresses SpRYc-ABE8e in mammalian cells

    Depositor

    Pranam Chatterjee

    Article

    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

    Insert

    ecTadA(8e)-nSpRYc
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Sc++ (1-1119), SpRY (1111-1368), D10A

    Promoter

    CMV

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTE5417_p3WJdB_promoter dropout vector

    Plasmid

    #221191

    Purpose

    Promoter probe vector with dropout sequence for promoter sequences with 3WJdB reporter

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Type

    Empty backbone

    Use

    Synthetic Biology

    Promoter

    Promoter Placeholder/Dropout Vector

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME_Cp_0_3b_026_Flag-tag

    Plasmid

    #235879

    Purpose

    FLAG N-tag

    Depositor

    Tobias Erb

    Insert

    FLAG N-tag
    

    Use

    Synthetic Biology

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME_Cp_0_3b_031_mVenus

    Plasmid

    #235884

    Purpose

    mVenus N-tag

    Depositor

    Tobias Erb

    Insert

    mVenus N-tag
    

    Use

    Synthetic Biology

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pME_Cp_0_3b_030_mCerulean

    Plasmid

    #235883

    Purpose

    mCerulean N-tag

    Depositor

    Tobias Erb

    Insert

    mCerulean N-tag
    

    Use

    Synthetic Biology

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVSV-G

    Plasmid

    #138479

    Purpose

    Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    VSV-G envelop protein
    

    Expression

    Mammalian

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-AmCyan

    Plasmid

    #138481

    Purpose

    For cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    AmCyan
    

    Expression

    Mammalian

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PL-5LTR-GW-A

    Plasmid

    #138480

    Purpose

    For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
    

    Expression

    Mammalian

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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