We narrowed to 829 results for: TG

1. pLenti-Blast-sgAMPKa1

   Plasmid

   #138704

   Purpose

   Expresses a human AMPKa1-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgAMPKa1 human (PRKAA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. sFlt1-e15a V5

   Plasmid

   #86045

   Purpose

   human Soluble Flt1 isoform e15a (terminates in exon 15a)

   Depositor

   Christie Thomas

   Article

   Thomas et al J Clin Endocrinol Metab. 2009 Jul;94(7):

   Insert

   human soluble Flt1 isoform e15a (FLT1 Human)
   

   Use

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   isoform e15a (terminates in exon 15a)

   Promoter

   CMV

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40

   Plasmid

   #101061

   Purpose

   AAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Has Service

   AAV1

   Insert

   CaMPARI2-F391W
   

   Use

   AAV

   Tags

   FLAG-HA-myc and NES_his

   Expression

   Mutation

   Promoter

   hsyn (synapsin-1)

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. p-mCherry-C1-iC++_CA

   Plasmid

   #98168

   Purpose

   slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with cyan light, inactivation max with 590 nm (accelarates closure to ms). Codon optimized for mammalian expression.

   Depositor

   Peter Hegemann

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct iC++_C128A gene
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A, V24…

   Promoter

   CMV (+enhancer)

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-Puro-sgAMPKa1

   Plasmid

   #138684

   Purpose

   Expresses a human AMPKa1-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgAMPKa1 human (PRKAA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V

   Plasmid

   #119723

   Purpose

   AAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Thomas Oertner

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Inserts

   CaMPARI2
   CaMPARI2
   

   Use

   AAV

   Tags

   NES-his

   Expression

   Mammalian

   Mutation

   F391W, L398V

   Promoter

   hSyn1

   Available Since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRetroX-TRE3G_FLAG-CRTC1/MAML2_PGK-GpNLuc

   Plasmid

   #154265

   Purpose

   Dox/Tet-inducible expression of the CRTC1-MAML2 fusion oncogene in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

   Insert

   FLAG-CRTC1/MAML2 fusion oncogene
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p-mCherry-C1-Phobos_CA

   Plasmid

   #98169

   Purpose

   slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.

   Depositor

   Peter Hegemann

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct Phobos_C128A gene
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…

   Promoter

   CMV (+enhancer)

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40

   Plasmid

   #101062

   Purpose

   AAV vector expressing CaMPARI2_H396K (Kd = 360nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Insert

   CaMPARI2-H396K
   

   Use

   AAV

   Tags

   FLAG-HA-myc and NES_his

   Expression

   Mutation

   Promoter

   hsyn (synapsin-1)

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p-mCherry-C1-Aurora_CA

    Plasmid

    #98170

    Purpose

    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Aurora_C128A gene
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…

    Promoter

    CMV (+enhancer)

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV-TRE3G_3xFLAG-PGC1a4_CMV-mCherry:T2A:Hygro

    Plasmid

    #154263

    Purpose

    Dox/Tet-inducible expression of the Human PGC-1a4 splice variant in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    3xFLAG-PGC-1a4 splice variant (PPARGC1A Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV2_hSyn_Phobos_CA_Citrine

    Plasmid

    #98218

    Purpose

    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.

    Depositor

    Simon Wiegert

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Phobos_C128A gene
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…

    Promoter

    human synapsin

    Available Since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid

    #127668

    Purpose

    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and hU6

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MSCV-shCasp2-IRES-GFP

    Plasmid

    #52061

    Purpose

    stable knockdown of Caspase-2 in mammalian cells

    Depositor

    Tyler Jacks, Trudy Oliver

    Article

    Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

    Insert

    Caspase-2 shRNA (CASP2 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer

    Plasmid

    #86195

    Purpose

    U6 based expression of N meningitidis sgRNA

    Depositor

    Scot Wolfe

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    nmCas9 sgRNA cloning cassete
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LADL Bridge + Promoter Only Target

    Plasmid

    #127669

    Purpose

    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    CRY2-HA-2A-mCherry and gRNAs 115 and 117
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and hU6

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Control-TP53-LRG-GFP

    Plasmid

    #225874

    Purpose

    Guides targeting P53 and a control sequence

    Depositor

    Janai Carr-Ascher

    Article

    Freeland et al Clin Cancer Res. 2024 Aug 23. doi: 10.11

    Insert

    Control and TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDH_CMV_dHMGA_mKate2_CLEAVAGE

    Plasmid

    #167337

    Purpose

    Expresses mKate2 fused to TFAM dHMGA mutant in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Lentiviral

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Truncation mutant containing MTS (1-42) as well a…

    Promoter

    CMV

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDH_CMV_HMGB_ctail_mKate2_CLEAVAGE

    Plasmid

    #167338

    Purpose

    Expresses mKate2 fused to TFAM HMGB+C-tail mutant in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Lentiviral

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Truncation mutant containing MTS (1-42) as well a…

    Promoter

    CMV

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV2_hSyn_Aurora_CA_Citrine

    Plasmid

    #98219

    Purpose

    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.

    Depositor

    Simon Wiegert

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Aurora_C128A gene
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…

    Promoter

    human synapsin

    Available Since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge