Addgene

We narrowed to 3,628 results for: WOL

Showing: 381 - 400 of 3628 results

Results per page:

Sort results by:

pAAVS1-Nst-CAG-DEST

Plasmid

#80489

Purpose

Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-OTUB1 C91S-pcDNA3.1

Plasmid

#118210

Purpose

Expresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

changed cysteine 91 to serine

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-TAG-ERN

Plasmid

#80476

Purpose

All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and neomycin resistance)

Depositor

Knut Woltjen

Article

Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

Type

Empty backbone

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
LbCas12a-2C-NLS in pCSDest

Plasmid

#126638

Purpose

Expresses LbCas12a-2C-NLS in mammalian cell

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

LbCas12a-2C-NLS

Use

CRISPR

Tags

3xHuman influenza hemagglutinin (HA)-Tag

Expression

Mammalian

Mutation

Promoter

CMV IE94 promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-21a-PE2-His

Plasmid

#201973

Purpose

Express His-tagged PE2 in bacteria

Depositor

Scot Wolfe

Article

Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

Insert

PE2-HIS

Use

Tags

His and c-myc NLS

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPBT-PE2-PGK-Blast

Plasmid

#173219

Purpose

piggyBac transposon containing PE2 and Blasticidin expression cassettes

Depositor

Jacob Giehm Mikkelsen

Article

Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

Inserts

PE2
Blast

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

CMV and hPGK

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-OTUB1 T134R-pcDNA3.1

Plasmid

#118211

Purpose

Expresses FLAG-OTUB1 T134R mutant in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

changed threonine 134 to arginine

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-TET-mSox2

Plasmid

#20908

Purpose

piggyBac vector with tet inducible mSox2

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

Sox2 (Sox2 Mouse)

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-21a_2xNLS_LbCpf1_protein_expression

Plasmid

#114366

Purpose

Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

Depositor

Scot Wolfe

Article

Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

Insert

2xNLS_LbCpf1

Use

CRISPR

Tags

NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEW3: pET-32a Dot1L(2-416)

Plasmid

#124098

Purpose

Dot1L(2-416) expression plasmid

Depositor

Cynthia Wolberger

Article

Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

Insert

Dot1L(2-416) (DOT1L Human)

Use

Tags

Trx-His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPBT-peRNA_GG-Puro

Plasmid

#173220

Purpose

piggyBac transposon containing hU6 promotor and pegRNA cloning site

Depositor

Jacob Giehm Mikkelsen

Article

Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

Inserts

pegRNA cloning cassette
PuroR

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EF1-a and hU6

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pB1H1-Zif268

Plasmid

#12612

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

Insert

Zif268 in pB1H1 (Egr1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
SpyCas9 EMX1-nicking sgRNA

Plasmid

#169859

Purpose

SpyCas9 nicking sgRNA for EMX1

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SpyCas9 EMX1-nicking sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AsCas12a-DR-cr in PBCSK-

Plasmid

#126640

Purpose

Cloning vector AsCas12a DR-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

AsCas12a Full Length Direct Repeat crRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-ING1b

Plasmid

#79052

Purpose

expression of p33ING1b in mammalian cells

Depositor

Karl Riabowol

Article

Scott et al J Cell Sci. 2001 Oct;114(Pt 19):3455-62.

Insert

ING1 (p33 ING1Ab isoform) (ING1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only
pRCP

Plasmid

#195862

Purpose

Encodes chromogenic protein (CP). CP inducible with rhamnose.

Depositor

Gregory Phillips

Article

DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

Insert

Chromogenic Protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-21a_FnCpf1_2xNLS_protein_expression

Plasmid

#124548

Purpose

Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

FnCpf1_2xNLS

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-HA-GAAZFP-FokI-RR

Plasmid

#18754

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

zinc finger nuclease

Use

Tags

Expression

Bacterial

Mutation

RNA expression vector for expressing the zinc fin…

Promoter

Available since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
SauCas9 EMX1-nicking sgRNA

Plasmid

#169860

Purpose

SauCas9 nicking sgRNA for EMX1

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SauCas9 EMX1-nicking sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEW4: pET-32a Dot1L(2-386)

Plasmid

#124099

Purpose

Dot1L(2-386) expression plasmid

Depositor

Cynthia Wolberger

Article

Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

Insert

Dot1L(2-386) (DOT1L Human)

Use

Tags

Trx-His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge