We narrowed to 29,553 results for: des.2

1. pBS_KS_attB2_SA(2)_T2AGAL4

   Plasmid

   #125213

   Purpose

   Artificial phase 2 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

   Depositor

   Herman Dierick

   Article

   Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

   Insert

   GAL4
   

   Use

   Other

   Tags

   T2A

   Available Since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPTK003-2-pENO1

   Plasmid

   #84962

   Purpose

   Type 2, pENO1, Enolase 1 promoter

   Depositor

   Volker Sieber

   Article

   Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

   Insert

   Enolase 1 promoter
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   BsaI site removed (2350 g to c) and BsmBI site re…

   Promoter

   Non

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSico Spry-2

   Plasmid

   #14809

   Depositor

   Tyler Jacks

   Article

   Shaw et al Genes Dev. 2007 Mar 15. 21(6):694-707.

   Insert

   Sprouty-2 shRNA (Spry2 Mouse)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Available Since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pJMC-2-Reverse

   Plasmid

   #197801

   Purpose

   Level 1 Module 2 destination vector (reverse orientation MoClo insert)

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDTnLAPP2A frame 2

   Plasmid

   #28228

   Depositor

   Harald von Melchner

   Article

   Schnutgen et al Nat Methods. 2011 Feb . 8(2):103-4.

   Insert

   Hygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein
   

   Use

   Retroviral

   Tags

   EGFP and nLAP-tag

   Available Since

   Sept. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. Hv_HPT:pCER6#2:Gus_introns

   Plasmid

   #215200

   Purpose

   Evaluating guard cell specific promotors in Barley

   Depositor

   Penny Hundleby

   Article

   Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

   Insert

   3-ketoacyl-coa synthase6
   

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   HvCER6#2

   Available Since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAP-U2-2

   Plasmid

   #236473

   Purpose

   Plasmid for integration at the native URA3 locus with 3xmCherry under control of the SpH2B promoter and 3xGFP under control of the ScACT1 promoter.

   Depositor

   Daniel Lew

   Article

   Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

   Insert

   spH2Bp-3xmCherry scACT1p-3xGFP
   

   Expression

   Yeast

   Mutation

   Codon optimized for A. pullulans

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pML104-LEU2-2

   Plasmid

   #226263

   Purpose

   Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene

   Depositor

   Jolanda Van Leeuwen

   Article

   Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

   Insert

   LEU2 gRNA (LEU2 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459-homo_PTGS1-2

   Plasmid

   #184732

   Purpose

   COX1-KO in HeLa

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   A gRNA targeting the human COX1 gene.
   

   Use

   CRISPR

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459-homo_PLA2G4A-2

    Plasmid

    #184736

    Purpose

    cPLA2-KO in HeLa

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    A gRNA targeting the human cPLA2 gene.
    

    Use

    CRISPR

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJMC-2-BsaI

    Plasmid

    #197818

    Purpose

    Level 1 module 2 with BsaI expansion point

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    None
    

    Use

    Unspecified

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJMC-2-Esp3I

    Plasmid

    #197830

    Purpose

    Level 1 module 2 with Esp3I expansion point

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    None
    

    Use

    Unspecified

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Ssh1 gRNA#2

    Plasmid

    #163395

    Purpose

    Cas9-mediated knockout of Ssh1 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    ssh1 (Ssh1 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ssh2 gRNA#2

    Plasmid

    #163398

    Purpose

    Cas9-mediated knockout of Ssh2 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Ssh2 (Ssh2 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ssh3 gRNA#2

    Plasmid

    #163401

    Purpose

    Cas9-mediated knockout of Ssh3 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Ssh3 (Ssh3 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBzCas13b-gRNA-2

    Plasmid

    #164859

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-2
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLsCas13a-gRNA-2

    Plasmid

    #164866

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-2
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBS_KS_attB2_SA(2)_T2AGeneSwitch

    Plasmid

    #125216

    Purpose

    Artificial phase 2 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GeneSwitch
    

    Use

    Other

    Tags

    T2A

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBS_KS_attB2_SA(2)_T2AGAL80

    Plasmid

    #125219

    Purpose

    Artificial phase 2 exon to include a spliced T2A-GAL80 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GAL80
    

    Use

    Other

    Tags

    T2A

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. HM110 prp16-2

    Plasmid

    #111277

    Purpose

    prp16-2 in pSE358(Trp)

    Depositor

    Christine Guthrie

    Article

    Madhani et al Genetics. 1994 Jul;137(3):677-87.

    Insert

    PRP16
    

    Expression

    Yeast

    Mutation

    prp-2 (D575N)

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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