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pET28a-SpyTagMBP

Plasmid

#35050

Depositor

Mark Howarth

Publication

Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

Insert

SpyTagMBP

Tags

His6

Expression

Bacterial

Mutation

Simple peptide tag added to the N-terminus of MBP…

Promoter

T7 promoter

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pKJ1712

Plasmid

#21870

Depositor

Keith Joung

Publication

Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pKM464 (deletions)

Plasmid

#108322

Purpose

Orbit integrating plasmid to delete target gene.

Depositor

Kenan Murphy

Publication

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

Bxb1 attB site

Use

Orbit integration plasmid for creating knockouts …

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pRRL-MHCK7-GCaMP6

Plasmid

#65042

Purpose

Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

Depositor

Nenad Bursac

Publication

Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

Insert

GCaMP6

Use

Lentiviral

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Promoter

MHCK7

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pDD284

Plasmid

#66825

Purpose

TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Publication

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

TagRFP-T-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized TagRFP-T

Expression

Worm

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pRSET-6xTR-TUBE

Plasmid

#110313

Purpose

E. coli expression and purification of 6xTR-TUBE

Depositor

Yasushi Saeki

Publication

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

6xTR-TUBE (UBQLN1 Human)

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3.1-hLbCpf1(TYCV) (pY230)

Plasmid

#89355

Purpose

Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

Depositor

Feng Zhang

Publication

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

G532R/K595R

Promoter

CMV

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHSP02

Plasmid

#117259

Purpose

Genome editing for Lactobacillus plantarum WCSF1

Depositor

Sheng Yang

Publication

Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

Insert

Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537

Use

Other

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pH-ePPE

Plasmid

#183097

Purpose

For plant prime editing in rice plants

Depositor

Caixia Gao

Publication

Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

Insert

Nls-nCas9-NC-nls-MLV-Nls

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV-CMV-SOD2-2A-Catalase-WPRE

Plasmid

#67635

Purpose

AAV vector expressing both SOD2 and mitochondrial targeted Catalase

Depositor

Connie Cepko

Publication

Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

Insert

SOD-2A-Catalase (Sod2, Cat Mouse)

Use

AAV

Mutation

Deleted the Peroxisome Targeting Signal from Cata…

Promoter

CMV

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PT3.5/CMV-EGFRvIII

Plasmid

#20280

Depositor

John Ohlfest

Publication

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

EGFRvII (EGFR Human)

Use

Transposon

Mutation

See depositor comments below.

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PX404 Campylobacter jejuni Cas9

Plasmid

#68338

Purpose

Campylobacter jejuni Cas9

Depositor

Feng Zhang

Insert

CjCas9

Use

CRISPR

Tags

HA-NLS and NLS

Expression

Mammalian

Promoter

CMV

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pmIL-6 FL

Plasmid

#61286

Purpose

drives luciferase from Wt mouse IL-6 promoter (full length)

Depositor

Gail Bishop

Publication

Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

Insert

IL-6 promoter (Il6 Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

IL-6 full length promoter

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pTAT-HA

Plasmid

#35612

Depositor

Steven Dowdy

Publication

Nagahara et al Nat Med. 1998 Dec;4(12):1449-52.

Type

Empty backbone

Tags

6xHIS, HA, and TAT

Expression

Bacterial

Promoter

T7

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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Mouse metabolism knockout library

Pooled library

#160129

Purpose

Targets mouse metabolic genes and includes most metabolic enzymes and transporters.

Depositor

Kivanc Birsoy

Publication

Zhu et al Cell Metab. 2020 Oct 28. pii: S1550-4131

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)

Plasmid

#199604

Purpose

Expression of cell permeable spCas9 in E. Coli.

Depositor

Junwei Shi

Publication

Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

Insert

Cas9-T6N

Use

CRISPR

Expression

Bacterial

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-sACE2(WT)-Fc(IgG1)

Plasmid

#145163

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1)

Depositor

Erik Procko

Publication

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pX330a dCas9-LSD1

Plasmid

#92362

Purpose

Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Publication

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9-LSD1

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJW1219

Plasmid

#61250

Purpose

CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans

Depositor

Jordan Ward

Publication

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

sgRNA(F+E)

Use

CRISPR

Expression

Worm

Promoter

U6 promoter

Available since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCMV-PTEN

Plasmid

#28298

Depositor

Maria-Magdalena Georgescu

Publication

Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

Insert

PTEN (PTEN Human)

Tags

FLAG

Expression

Mammalian

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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