We narrowed to 62,258 results for: cloning

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Notch3 intracellular domain-pcw107-V5

Plasmid

#64623

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH3 (NOTCH3 Human)

Use

Lentiviral

Tags

V5

Mutation

intracellular domain *

Promoter

PGK

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
GFP-PHIP

Plasmid

#65389

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

PHIP (PHIP Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
Rasgrf2-2A-dCre targeting vector

Plasmid

#61573

Purpose

Target a DHFR-destabilized Cre recombinase gene to the stop codon of the mouse Rasgrf2 gene

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Rasgrf2-2A-dCre (Rasgrf2 P1 bacteriophage, Mouse)

Use

Cre/Lox and Mouse Targeting

Available Since

March 31, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiX2-PURO-shLKB1-Hu

Plasmid

#61242

Purpose

Lentivirus expressing shRNA to human LKB1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

LKB1 (STK11 Human)

Use

Lentiviral

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1511

Plasmid

#62365

Purpose

pEF1a-puro-T2A-ATHmmuRPL10A

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

Puro-T2A-AVITEVHAmmuRPL10A

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pDCC5

Plasmid

#59984

Purpose

Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

Depositor

Peter Duchek

Article

Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

Insert

Cas9-D10A

Use

CRISPR

Tags

HA

Expression

Insect

Mutation

D10A

Promoter

hsp70Bb

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHAhFMRPiso1I304N

Plasmid

#48692

Purpose

Expresses hFMRP iso 1 I304N

Depositor

Thomas Tuschl

Article

Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

Insert

FlagHA FMR1 Iso 1 I304N (FMR1 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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Active Wnt2-V5

Plasmid

#43809

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT2 (WNT2 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-N 16E5

Plasmid

#37874

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 16 E5 (E5 )

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

July 25, 2012

Availability

Academic Institutions and Nonprofits only
LHP1481 - TOPO-ProteinA

Plasmid

#32225

Depositor

Linda Hicke

Insert

Protein A

Use

Cloning

Promoter

P-lac

Available Since

Oct. 24, 2011

Availability

Academic Institutions and Nonprofits only
pLenti6/V5-DEST-FASCIN

Plasmid

#31207

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

FASCIN (FSCN1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pLD-puro-CcVA

Plasmid

#24588

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-ABL2

Plasmid

#23909

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ABL2 (ABL2 Human)

Use

Gateway Cloning

Mutation

K115M

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MST1R

Plasmid

#23942

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MST1R (MST1R Human)

Use

Gateway Cloning

Mutation

R322Q

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR221-hSOX10

Plasmid

#24749

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway Cloning

Mutation

wildtype sequence

Available Since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
pJA245

Plasmid

#21506

Depositor

Julie Ahringer

Article

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

pmex-5::GFP (w introns)::(Gly)5Ala

Use

Gateway Cloning

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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MS-1 magA

Plasmid

#21751

Depositor

Elliot Meyerowitz

Article

Bertani et al Gene. 2001 Feb 21. 264(2):257-63.

Insert

magA (magA M. magnetotacticum)

Use

Cloning

Available Since

Aug. 21, 2009

Availability

Academic Institutions and Nonprofits only
pENTR4-HA-myc-nuc (w557-1)

Plasmid

#20025

Purpose

Gateway entry vector for nuclear targeting of domains (N-term HA , C-term NLS, myc tags)

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

HA, Myc, and NLS

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pCG144

Plasmid

#21376

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

mCherry in pDONR201

Use

Gateway Cloning

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pDEST12.2 DYRK2 WT

Plasmid

#20005

Depositor

Anjana Rao

Article

Gwack et al Nature. 2006 Jun 1. 441(7093):646-50.

Insert

DYRK2 (DYRK2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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