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pcDNA3.2-Cx43-M100/125/147/213-siResist

Plasmid

#49858

Purpose

Encodes human connexin 43 with M100, M125, M147, and M213 mutated to L and wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Expression

Mammalian

Mutation

Methionine100, Methionine 125, Methionine 147, an…

Promoter

CMV

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K5S-Mt206B

Plasmid

#34600

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

The KLF4 K5S region was mutated at the miR-206 bi…

Promoter

CMV

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
pENTR223-PARK7H126A

Plasmid

#115180

Purpose

Gateway cloning of PARK7H126A into any destination vector

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7H126A (PARK7 Human)

Use

Gateway cloning into appropriate destination vect…

Mutation

p.H126A

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR223-PARK7E163K

Plasmid

#115181

Purpose

Gateway cloning of PARK7E163K into any destination vector

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7E163K (PARK7 Human)

Use

Gateway cloning into appropriate destination vect…

Mutation

p.E163K

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2

Plasmid

#115192

Purpose

Lentiviral transduction and expression of PDHA2 into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PDHA2WT (PDHA2 Human)

Use

Lentiviral

Promoter

EF1alpha

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2S291A

Plasmid

#115196

Purpose

Lentiviral transduction and expression of PDHA2S291A into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PDHA2S291A (PDHA2 Human)

Use

Lentiviral

Mutation

p.S291A

Promoter

EF1alpha

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2S291A/S293A

Plasmid

#115198

Purpose

Lentiviral transduction and expression of PDHA2S291A/S293A into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PDHA2S291A/S293A (PDHA2 Human)

Use

Lentiviral

Mutation

p.S291A/S293A

Promoter

EF1alpha

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR223-PARK7V51G

Plasmid

#115178

Purpose

Gateway cloning of PARK7V51G into any destination vector

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7V51G (PARK7 Human)

Use

Gateway cloning into appropriate destination vect…

Mutation

p.V51G

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR223-PARK7C53A

Plasmid

#115179

Purpose

Gateway cloning of PARK7C53G into any destination vector

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7C53A (PARK7 Human)

Use

Gateway cloning into appropriate destination vect…

Mutation

p.C53A

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPHAGE-EF1α-Luc-C20orf24-3’UTR-VAR

Plasmid

#128507

Purpose

Lentiviral constitutive expression of Firefly luciferase under control of 3'UTR of human C20orf24 with variant from COX deficiency patient.

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

Firefly luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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RCH1:HDT1,HDT2-RNAi

Plasmid

#108446

Purpose

Arabidopsis transformation, RNAi vector targeting HDT1/HDT2 with RCH1 promoter

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Inserts

HDT1 (HDA3 Mustard Weed)
HDT2 (HD2B Mustard Weed)

Use

RNAi

Expression

Plant

Promoter

RCH1

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only
pBEST-p70a-UTR1-deGFP Y35TAG D193TAG-6xHis-T500

Plasmid

#92227

Purpose

Expression of deGFP mutant Y35TAG D193TAG for ncAA incoporation using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

deGFP Y35TAG D193TAG

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Mutation

Residues Y35 D193 were mutated into TAG (Amber) s…

Promoter

p70a

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pTH748-CEN-RLuc/min8maxCFLuc

Plasmid

#38214

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

The first eight codons of the original FLuc gene …

Promoter

ADH1 and TDH3 (=GDP)

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pTH753-CEN-RLuc/min346maxCFLuc

Plasmid

#38219

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

The first 346 codons of the original FLuc gene we…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pTH747-CEN-RLuc/min4maxCFLuc

Plasmid

#38213

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

The first four codons of the original FLuc gene w…

Promoter

ADH1 and TDH3 (=GDP)

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-Luc-KLF4-K5S-Mt344B

Plasmid

#34602

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

The KLF4 K5S region was mutated at the miR-344 bi…

Promoter

CMV

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K5S-Mt206A

Plasmid

#34599

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

The KLF4 K5S region was mutated at the miR-206 bi…

Promoter

CMV

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
pZE21-GFPaav

Plasmid

#26643

Depositor

Michael Elowitz

Article

Elowitz et al Nature. 2000 Jan 20. 403(6767):335-8.

Insert

pZE21-GFPaav

Tags

GFPaav

Expression

Bacterial

Mutation

na

Available Since

June 22, 2011

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-Fluc-HhR_E

Plasmid

#146247

Purpose

Insect Expression of HhR

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jan . 15(1):21-32.

Insert

HhR

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-Fluc-HSL_E

Plasmid

#146248

Purpose

Insect Expression of HSL

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jan . 15(1):21-32.

Insert

HSL

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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