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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,508 results for: crispr

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Showing: 4021 - 4040 of 25508 results

  10. pCMV-BE-VQRCas9

    Plasmid
    #101740
    Purpose
    expression of an optimized Cas9 for base-editing in zebrafish
    Depositor
    Shuo Lin
    Publication
    Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, VQR mutations
    Promoter
    CMV
    Available since
    Jan. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101740-plasmid-map-sequence-id-198315

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  12. pJK422

    Plasmid
    #155373
    Purpose
    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)
    Depositor
    Pamela Silver
    Publication
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Available since
    Dec. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    155373-plasmid-map-sequence-id-319760

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  13. pJK423

    Plasmid
    #155374
    Purpose
    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)
    Depositor
    Pamela Silver
    Publication
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Available since
    Dec. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    155374-plasmid-map-sequence-id-319753

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  14. pJC2528 - CLYBL-Neo-CAGGS-LSL-dCas9-TagBFP-KRAB-CLYBL (LSL-CRISPRi)

    Plasmid
    #250253
    Purpose
    Homology donor for knock-in of CRISPRi system into CLYBL locus with addgene plasmids 62196 and 62197. Lox-Stop-Lox requires cre delivery for activation.
    Depositor
    Jacob Corn
    Publication
    Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:
    Insert
    dCas9-mTagBFP-KRAB
    Use
    CRISPR
    Tags
    HA-2xSV40 NLS-mTagBFP-KRAB
    Expression
    Mammalian
    Promoter
    CAGGS
    Available since
    Jan. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    250253-plasmid-map-sequence-id-498726

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  15. SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

    Plasmid
    #123615
    Purpose
    CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3(R33A/K34A)-P2A-EGFP
    Expression
    Mammalian
    Mutation
    R33A/K34A in rAPOBEC1, D10A in SpCas9
    Promoter
    CAG
    Available since
    April 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    123615-plasmid-map-sequence-id-239343

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  16. Cas9/pTREX-n

    Plasmid
    #68708
    Purpose
    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.
    Depositor
    Roberto Docampo
    Publication
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    Use
    CRISPR
    Tags
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …
    Mutation
    C4239T mutation that eliminates a BamHI restricti…
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68708-plasmid-map-sequence-id-123989

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Showing: 4021 - 4040 of 25508 results