We narrowed to 4,523 results for: ARA-2

1. AAV-CMV-scFVtet2_bGHpA

   Plasmid

   #177352

   Purpose

   AAV expression of scFV-fused catalytic domain of TET2 for targeted DNA methylation editing

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   Tet Methylcytosine Dioxygenase 2 (TET2 Human)
   

   Use

   AAV

   Tags

   myc and scFV

   Expression

   Mammalian

   Mutation

   Catalytic domains of human TET2 (1129–1936 and 14…

   Promoter

   CMV promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-hSyn-scFVtet2_bGHpA

   Plasmid

   #177353

   Purpose

   AAV expression of scFV-fused catalytic domain of TET2 from Synapisin promoter for targeted DNA methylation editing

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   Tet Methylcytosine Dioxygenase 2 (TET2 Human)
   

   Use

   AAV

   Tags

   myc and scFV

   Expression

   Mammalian

   Mutation

   Catalytic domains of human TET2 (1129–1936 and 14…

   Promoter

   human Synapsine promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-TRE-scFVtet2_bGHpA

   Plasmid

   #177358

   Purpose

   AAV expression of scFV-fused catalytic domain of TET2 from doxycycine-inducible promoter for targeted DNA methylation editing

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   Tet Methylcytosine Dioxygenase 2 (TET2 Human)
   

   Use

   AAV

   Tags

   myc and scFV

   Expression

   Mammalian

   Mutation

   Catalytic domains of human TET2 (1129–1936 and 14…

   Promoter

   Tetracycline-dependent promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMeCP2G pc1121

   Plasmid

   #167566

   Purpose

   The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced GFP (isolated from pEGFP-N1 vector; CLONTECH Laboratories, Inc.)

   Depositor

   Cristina Cardoso

   Article

   Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

   Inserts

   Methyl-CpG binding protein 2 (Mecp2 Rat)
   GFP
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONOR-SNCAe2-WT

   Plasmid

   #85845

   Purpose

   Donor plasmid for SNCA exon2 wild type sequence. Also contains TagBFP and EGFP

   Depositor

   Jens Schwamborn

   Article

   Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

   Insert

   SNCA exon 2 homology arms (SNCA Human)
   

   Tags

   -

   Expression

   Bacterial and Mammalian

   Mutation

   -

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4/TO h-Stx3 L165A/E166A-2xMyc/His

   Plasmid

   #99743

   Purpose

   Expresses human Stx3 locked into the open conformation with a C-term myc-myc-his tag in mammalian cells.

   Depositor

   Thomas Weimbs

   Article

   Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

   Insert

   Stx3 (STX3 Human)
   

   Tags

   myc-myc-his

   Expression

   Mammalian

   Mutation

   Changed both L165 and E166 to Alanine

   Promoter

   CMV

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TO-hNIL BSD-mApple

   Plasmid

   #182313

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression, bsd selection, mApple

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   hNGN2 (NEUROG2 Human)
   hISL1 (ISL1 Human)
   hLHX3 (LHX3 Human)
   bsd-T2A-mycNLS-mApple
   

   Tags

   T2A-mycNLS-mApple

   Expression

   Mammalian

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIRES-mCerulean-GAI1-92-KRas/N12-KRas/13C(Q61L)-GID1-mVenus-CAAX

   Plasmid

   #232262

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

   Inserts

   mCerulean-GAI1-92-KRas/N12 (KRAS Human)
   KRas/13C(Q61L)-GID1-mVenus-CAAX (KRAS Human)
   

   Tags

   mCerulean and mVenus

   Expression

   Mammalian

   Mutation

   KRas-Q61L

   Promoter

   CMV

   Available Since

   Sept. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIRES-mCerulean-GAI1-92-KRas/N12-KRas/13C(S17N)-GID1-mVenus-CAAX

   Plasmid

   #232263

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

   Inserts

   mCerulean-GAI1-92-KRas/N12 (KRAS Human)
   KRas/13C(S17N)-GID1-mVenus-CAAX (KRAS Human)
   

   Tags

   mCerulean and mVenus

   Expression

   Mammalian

   Mutation

   KRas-S17N

   Promoter

   CMV

   Available Since

   Sept. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHIS2-hsSTAM1 (1-143)

    Plasmid

    #21498

    Depositor

    James Hurley

    Article

    Ren et al Structure. 2009 Mar 11. 17(3):406-16.

    Insert

    phis2- STAM1(1-143) (STAM Human)
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

11. LV-AR-C619Y

    Plasmid

    #171222

    Purpose

    Lenti-viral expression of human androgen receptor DNA-binding mutant: C619Y, mutation at the end of zinc finger 2.

    Depositor

    Steven Balk

    Article

    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

    Insert

    AR-C619Y (AR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-C619Y

    Promoter

    EF-1a

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEVOL-pylT-AznLRS

    Plasmid

    #172482

    Purpose

    For E.Coli expression, 306L309L348I384F411W

    Depositor

    Wenshe Liu

    Article

    Wang et al Angew Chem Int Ed Engl. 2017 Feb 1;56(6)

    Insert

    AznLRS and pylTcua
    

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. YFP-Rac2(N17)

    Plasmid

    #11398

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    ras-related C3 botulinum toxin substrate 2 (RAC2 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Obtained with Threonine 17 changed to Asparagine.

    Available Since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWA PAS-E BAD K-GAFm

    Plasmid

    #39866

    Depositor

    Vladislav Verkhusha

    Article

    Filonov et al Chem Biol. 2013 Jul 23. pii: S1074-5521(

    Inserts

    iRFP protein PAS domain
    iRFP protein GAF domain (mutated)
    

    Tags

    E coil and K coil

    Expression

    Bacterial

    Mutation

    F165Y, D232Y, W308R

    Promoter

    arabinose inducible and rhamnose inducible

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEN6-Ubc5 Sc

    Plasmid

    #83234

    Purpose

    pHis-par2-UbHs/Ubc5Sc/ Uba1Ta

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Inserts

    Ubiqutin (UBC Human)
    Ubc 5 (UBC5 Budding Yeast)
    Uba 1
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA5-LRRK2

    Plasmid

    #229019

    Purpose

    Expression of untagged full length human LRRK2 in mammalian cells

    Depositor

    Samara Reck-Peterson

    Article

    Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

    Insert

    Leucine-rich repeat kinase 2 (LRRK2 Human)
    

    Tags

    no tags (untagged)

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFP.E222

    Plasmid

    #138248

    Purpose

    Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    ECF20.N.(VDA).M86.N2
    Terminator
    SspGyrB.C2.(SAK).ECF16.C
    Terminator
    rhaS
    ECF17.N.(NPC).NrdJ-1.N2
    Terminator
    M86.C2.(SDL).ECF20.C
    Terminator
    luxR
    Terminator
    ECF16.N.(AGG).SspGyrB.N2
    Terminator
    NrdJ-1.C2.(SEI).ECF17.C
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. GSO2/EDA23 X079_pECIA2

    Plasmid

    #115111

    Purpose

    Bait vector GSO2/EDA23 X079_pECIA2 should be used with prey vector GSO2/EDA23 X079_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT5G44700 (GSO2 Mustard Weed)
    

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHis6-SUMO-g0s2

    Plasmid

    #179293

    Purpose

    E. coli expression plasmid (T7 promoter) for full-length human G0S2 with N-terminal His6 + SUMO + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do

    Insert

    G0S2 (G0S2 Human)
    

    Tags

    His6 + SUMO + TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    full-length G0S2, residues 1-103 from accession n…

    Promoter

    T7

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. MBP-g0s2

    Plasmid

    #179294

    Purpose

    E. coli expression plasmid (Ptac promoter) for full-length human G0S2 with N-terminal mature maltose binding protein + enterokinase cleavage site

    Depositor

    Debra Hansen

    Article

    Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do

    Insert

    G0S2 (G0S2 Human)
    

    Tags

    mature maltose binding protein + enterokinase cle…

    Expression

    Bacterial

    Mutation

    full-length G0S2, residues 1-103 from accession n…

    Promoter

    Ptac

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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