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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,282 results for: LEA

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Showing: 4041 - 4060 of 12282 results

  4. pcDNA3-CTE-Luc

    Plasmid
    #60881
    Purpose
    Reporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTE
    Depositor
    Vinay Nandicoori
    Publication
    Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Luc-CTE
    Expression
    Mammalian
    Available since
    Jan. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    60881-plasmid-map-sequence-id-98231

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  9. P9_TEVs_cLuc

    Plasmid
    #119303
    Purpose
    Expresses C-terminus of split luciferase connected to P9 coil via TEVs cleavable linker/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic
    Depositor
    Roman Jerala
    Publication
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    split cLuc connected to P9 coil via TEVs cleavable linker
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    119303-plasmid-map-sequence-id-231805

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  11. 4YWQ

    Plasmid
    #69953
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RC3H1:YTC019-B07:C232791 (RC3H1 Human)
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article

  12. GLYR1

    Plasmid
    #38946
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GLYR1 (GLYR1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pCW57.1 DOX off blast POLE

    Plasmid
    #241437
    Purpose
    Expresses DOX-suppressible POLE
    Depositor
    Richard Possemato
    Insert
    POLE (POLE Human)
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Silent Mutations disrupt shRNA targeting sites
    Available since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    241437-plasmid-map-sequence-id-492172

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  14. pHis-TEV-Nsp15

    Plasmid
    #187656
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Publication
    Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004
    Insert
    Nsp15 (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7lac
    Available since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    187656-plasmid-map-sequence-id-388768

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  15. pM9-BetaGal-8C

    Plasmid
    #172489
    Purpose
    Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence
    Depositor
    Siegfried Musser
    Publication
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    M9-BetaGal-8C
    Tags
    6xHis and M9 nuclear localization sequence (from …
    Expression
    Bacterial
    Mutation
    BetaGal has two cysteine mutations (C77A, C1022S)…
    Promoter
    T7
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    172489-plasmid-map-sequence-id-356067

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  16. NLS-4LNDD-pcDNA3.1(+)

    Plasmid
    #172313
    Purpose
    Expresses ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS) in mammalian cells
    Depositor
    Joshua Rosenthal
    Publication
    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:
    Insert
    Lambda N peptide - human ADAR2 Deaminase Domain
    Tags
    6xHis, Flag, and Nuclear Localization Signal of S…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    172313-plasmid-map-sequence-id-339585

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  17. pTE2690

    Plasmid
    #137717
    Purpose
    Expresses dimpLbCas12a-SunTag in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dimpLbCas12a
    Use
    CRISPR
    Tags
    NLSx3 SV40 and SunTagx10
    Expression
    Mammalian
    Mutation
    D156R, G532R, K538V, Y542R, K595R, D832A,
    Promoter
    CMV
    Available since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    137717-plasmid-map-sequence-id-274029

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  18. pcDNA3-JNKsubDD-YPet-NES

    Plasmid
    #84633
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Publication
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    JNKsubDD-YPet-NES
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    84633-plasmid-map-sequence-id-176152

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Showing: 4041 - 4060 of 12282 results