We narrowed to 15,991 results for: LEA

1. FUGW-VGLUT2-pHluorin

   Plasmid

   #207469

   Purpose

   Lentiviral expression of VGLUT2-pHluorin

   Depositor

   Robert Edwards

   Article

   Silm et al Neuron. 2019 May 22;102(4):786-800.e5. d

   Insert

   VGLUT2 (Slc17a6 Rat)
   

   Use

   Lentiviral

   Tags

   pHluorin

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-I-PhoCl-HCVp

   Plasmid

   #87697

   Purpose

   Express I-PhoCl-HCVp with N-term EBFP2 in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   I-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)
   

   Tags

   EBFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. ND4.2-Right DdCBE-G1397-C- T1413I

   Plasmid

   #179685

   Purpose

   Expression of base editor in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_SOD2 3'UTR
   

   Expression

   Mammalian

   Mutation

   T1413I

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-ERT2-PhoCl-Gal4VP16-PhoCl-ERT2

   Plasmid

   #87693

   Purpose

   Express ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   ERT2-PhoCl-Gal4VP16-PhoCl-ERT2
   

   Expression

   Mammalian

   Available Since

   May 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMJ826

   Plasmid

   #39316

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Histidine 840 to Alanine (H840A)

   Promoter

   T7

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD24-sfGFPx2

   Plasmid

   #51559

   Purpose

   Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)

   Depositor

   Francisco Malagon, Sankar Adhya

   Article

   Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

   Insert

   superfolder GFP
   

   Tags

   GLESTCRHASLAVLADERRFSA and MARARA

   Expression

   Bacterial

   Mutation

   Contains additonal aa in the N and C terminus (in…

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. Vinculin-venus

   Plasmid

   #27300

   Depositor

   Martin Schwartz

   Article

   Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

   Insert

   Vinculin (VCL Chicken)
   

   Tags

   Venus A206K

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDG04163

   Plasmid

   #216373

   Purpose

   xhNup214-Nb1t (+2Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xhNup214-Nb1t (+2Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-scSUMO

   Expression

   Bacterial

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-mPCSK4

   Plasmid

   #122675

   Purpose

   Expresses mouse PCSK4 in mammalian cells

   Depositor

   Liming Pei

   Article

   Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

   Insert

   Mus musculus proprotein convertase subtilisin/kexin type 4 (Pcsk4) (Pcsk4 Mouse)
   

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV Flag WT-PTEN

    Plasmid

    #22231

    Depositor

    Hong Wu

    Article

    Mosessian et al J Biol Chem. 2009 Oct 30;284(44):30159-6

    Insert

    PTEN (PTEN Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-Myc-Caspase-5

    Plasmid

    #214305

    Purpose

    Expression of gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP5 (CASP5 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Promoter

    MESV

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. WT PBRM1- TRIPZ-neo

    Plasmid

    #107406

    Purpose

    Mammalian expression, inducible PBRM1

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOCC151

    Plasmid

    #118899

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal GST, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-GST-3C-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    GST tag, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-GFP1-10-pDEST-C

    Plasmid

    #118369

    Purpose

    Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    HA-GFP1-10

    Expression

    Mammalian

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHJ421

    Plasmid

    #20680

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Ezrin (EZR Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSDM052

    Plasmid

    #194341

    Purpose

    pUC mini-Tn7 vector expressing LseCas13a

    Depositor

    Joseph Bondy-Denomy

    Article

    Mendoza et al Nature. 2020 Jan;577(7789):244-248. doi:

    Insert

    Cas13a(Lse)
    

    Expression

    Bacterial

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMJ841

    Plasmid

    #39318

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine (D10A) and Histidine 840 …

    Promoter

    T7

    Available Since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. 2HA-pDEST-C

    Plasmid

    #118374

    Purpose

    Gateway compatible mammalian expression vector with C-terminal 2HA to tag proteins of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    2HA

    Expression

    Mammalian

    Available Since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEYFP-C3-HA-Ubiquitin

    Plasmid

    #28236

    Depositor

    Michael Mancini

    Article

    Stenoien et al Nat Cell Biol. 2002 Oct . 4(10):806-10.

    Insert

    Ubiquitin (UBB Human)
    

    Tags

    EYFP and HA

    Expression

    Mammalian

    Available Since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPY22-mNeonGreen-Hyg

    Plasmid

    #137082

    Purpose

    Codon-optimized mNeonGreen for expression in Arabidopsis thaliana and Physcomitrella patens

    Depositor

    Gohta Goshima

    Article

    Yi et al Curr Biol. 2020 Jul 20;30(14):2860-2868.

    Insert

    mNeonGreen-Flag
    

    Expression

    Plant

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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