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pFETCh_FOXP1

Plasmid

#86261

Purpose

Donor vector for 3' FLAG tag of human FOXP1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

FOXP1 homology arms (FOXP1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCA528 SPASTIN , residues 108-200

Plasmid

#180628

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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SPLINTR BFP v1 backbone

Plasmid

#179779

Purpose

SPLINTR backbone vector

Depositor

Mark Dawson

Article

Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pRS313_ATP3

Plasmid

#120253

Purpose

Expression of ATP3 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Expression

Yeast

Promoter

ATP3

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pFETCh_KAT7

Plasmid

#86266

Purpose

Donor vector for 3' FLAG tag of human KAT7

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KAT7 homology arms (KAT7 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_FOXP1_1

Plasmid

#86299

Purpose

Encodes gRNA for 3' target of human FOXP1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against FOXP1 (FOXP1 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 MYC dLZ

Plasmid

#176048

Purpose

Expresses Myc Mutant (delta leucine zipper) in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Expression

Mammalian

Mutation

deleted amino acids 414-433

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pHS1068

Plasmid

#205962

Purpose

NZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promoters

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

DinG HNH proteins and CRISPR array

Expression

Bacterial

Promoter

Lac (proteins), pJ23119 (crRNA)

Available Since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV Puro SCRIB P305L

Plasmid

#88887

Purpose

SCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backbone

Depositor

Senthil Muthuswamy

Article

Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

Insert

SCRIB (SCRIB Human)

Use

Retroviral

Mutation

Proline to Leucine mutation @ 305th amino acid

Promoter

MSCV promoter

Available Since

March 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMS3: pHelper(AvCAST)_AvPSP1_ΔTnsD

Plasmid

#168135

Purpose

Inducible expression of AvCAST proteins (except TnsD) with crRNA targeting AvPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array (with spacer for AvPSP1)
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Oct4

Plasmid

#136611

Purpose

Dox-inducible lentiviral vector expressing mouse Oct4

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4 (Pou5f1 Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSCALPS-Flag-Mili-EGFP

Plasmid

#212576

Purpose

Lentiviral expression of Flag-tagged mouse Mili in mammalian cells

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

Mili (Piwil2 Mouse)

Use

Lentiviral

Tags

5x Flag and SNAP tag

Expression

Mammalian

Promoter

SFFV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMS21: pHelper(PmcCAST)_entry

Plasmid

#168154

Purpose

Inducible expression of PmcCAST proteins. Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-GFP-mSox2

Plasmid

#206374

Purpose

Expresses EGFP fused mouse SOX2 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox2 (Sox2 Mouse)

Use

Lentiviral

Tags

EGFP

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pFETCh_KDM6A

Plasmid

#86265

Purpose

Donor vector for 3' FLAG tag of human KDM6A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KDM6A homology arms (KDM6A Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_FOXP1_2

Plasmid

#86300

Purpose

Encodes gRNA for 3' target of human FOXP1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against FOXP1 (FOXP1 Human)

Use

CRISPR

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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Affibody EGFR:1907.BirA*-pET301/NT-DEST

Plasmid

#174691

Purpose

Plasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*

Depositor

Jean Gariepy

Article

Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

Insert

Anti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*

Tags

HIS tag

Expression

Bacterial

Mutation

R118G mutation in BirA

Promoter

T7 promoter

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pGS1022

Plasmid

#59576

Purpose

Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection marker

Depositor

Monika Golas, Bjoern Sander

Article

Rai et al Mol Biotechnol. 2014 Jun 27.

Insert

Twin-Strep tag optimized for PCR amplification followed by URA3 selection marker

Use

E. coli cloning vector

Tags

Twin-Strep tag

Promoter

None

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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BCL6-H2Kk-RV (H2K-BCL6)

Plasmid

#40352

Depositor

Alexander Dent

Article

Mondal et al J Immunol. 2010 Apr 15;184(8):4123-32. E

Insert

BCL6 (BCL6 Human)

Use

Retroviral

Tags

IRES mouse H2Kk

Expression

Mammalian

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pFETCh_CEBPG

Plasmid

#86285

Purpose

Donor vector for 3' FLAG tag of human CEBPG

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CEBPG homology arms (CEBPG Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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