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pECE-M2-BAIAP2 wt

Plasmid

#31656

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

BAI1-associated protein 2 (BAIAP2 Human)

Tags

M2

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pZW16 (CK2alpha')

Plasmid

#27087

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha' (CSNK2A2 Human)

Tags

HA

Expression

Mammalian

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only

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Venus-G protein Beta2 in pcDNA3.1

Plasmid

#42182

Depositor

Narasimhan Gautam

Article

O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

Insert

G protein Beta 2 (GNB2 Human)

Tags

Venus(156-239)

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-hGABARAP

Plasmid

#87871

Purpose

Express a EGFP-human GABARAP in mammalian cells

Depositor

Eiki Kominami, Isei Tanida

Article

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

GABARAP (GABARAP Human)

Expression

Mammalian

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-mGFAP-Lck-PinkFlamindo

Plasmid

#228394

Purpose

AAV vector to express the red cAMP indicator in astrocytes

Depositor

Tetsuya Kitaguchi

Article

Vittani et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

Pink Flamindo

Use

AAV

Promoter

mGFAP

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEHAC1-HA-BirA-GSGS-Traf6

Plasmid

#232586

Purpose

Express BirA tagged to the N-terminus of TRAF6 with a GSGS linker between BirA and TRAF6.

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

BirA -TRAF6 (TRAF6 Human)

Tags

HA

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

Plasmid

#134868

Purpose

Expresses EGFP-tagged lysosomal marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Lysosomal-associated membrane protein 1 (LAMP1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pKD279.8

Plasmid

#173171

Purpose

Encodes expression of SO_4388(REC)-PsdR(DBD) 137 and an sfGFP reporter

Depositor

Jeffrey Tabor

Article

Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

Inserts

SO_4388(REC)-PsdR(DBD) 137
sfGFP

Expression

Bacterial

Promoter

J23108 and PpsdA110

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV10-mApg16L

Plasmid

#24302

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Sci. 2003 May 1. 116(Pt 9):1679-8

Insert

autophagy related genes 16L (Atg16l1 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

V583M

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pZW12 (CK2beta)

Plasmid

#27088

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2beta (CSNK2B Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR wt-FLAG

Plasmid

#204532

Purpose

Mammalian expression of human CALR wt-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR wt-FLAG (CALR Human)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-hPPP3CB-FLAG

Plasmid

#179135

Purpose

Expression vector of human protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCIRCgp41-1

Plasmid

#74227

Purpose

Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

Depositor

Barbara Di Ventura, Roland Eils

Article

Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

Type

Empty backbone

Use

Synthetic Biology

Tags

gp41-1 C-intein and gp41-1 N-intein

Expression

Bacterial

Promoter

T7-lac

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-Calr-KDEL-IRES-Puromycin

Plasmid

#134664

Purpose

Expresses EGFP-tagged endoplasmic reticulum marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

calreticulin (CALR Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 18-413

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144~451-CMV2-mir1

Plasmid

#164664

Purpose

Express miR-144~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144~451 (MIR451A, MIR144 Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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YFP-UBF1-C1

Plasmid

#134532

Purpose

Expresses YFP-UBF1 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

UBF1 (UBTF Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin

Plasmid

#133027

Purpose

Expresses EGFP-tagged late endosome marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

RAB7A (RAB7A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

Human elongation factor 1 alpha

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pH4R-mCherry-N1

Plasmid

#84328

Purpose

Histamine-4 receptor tagged with mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H4R-mCherry (HRH4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-GRK3

Plasmid

#32689

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

Insert

GRK3 (GRK3 Bovine)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only
LENTICRISPR-SNRNP70_sgRNA1

Plasmid

#201620

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

SNRNP70 (SNRNP70 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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