We narrowed to 4,107 results for: lenti crispr plasmid

1. pEIF3D-S528D-S529D-sgEIF3D

   Plasmid

   #222127

   Purpose

   EIF3D variant overexpression vector with sgRNA targeting eIF3D

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   eIF3D (EIF3D Human)
   

   Use

   Lentiviral

   Tags

   BFP-P2A

   Mutation

   S528D, S529D

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEIF3D-NtermDel-CtermDel-sgEIF3D

   Plasmid

   #222131

   Purpose

   EIF3D variant overexpression vector with sgRNA targeting eIF3D

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   eIF3D (EIF3D Human)
   

   Use

   Lentiviral

   Tags

   BFP-P2A

   Mutation

   161 + 527 truncation

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEIF3D-S528N-S529N-sgEIF3D

   Plasmid

   #222129

   Purpose

   EIF3D variant overexpression vector with sgRNA targeting eIF3D

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   eIF3D (EIF3D Human)
   

   Use

   Lentiviral

   Tags

   BFP-P2A

   Mutation

   S528N, S529N

   Available Since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. UCK2_Deletion_gRNA_1

   Plasmid

   #204672

   Purpose

   Dual gRNA plasmid for UCK2 deletion

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   uridine-cytidine kinase 2 (UCK2 Human)
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. UCK2_Deletion_gRNA_2

   Plasmid

   #204673

   Purpose

   Dual gRNA plasmid for UCK2 deletion

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   uridine-cytidine kinase 2 (UCK2 Human)
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV_Donor/Reporter-COL4A5

   Plasmid

   #130280

   Purpose

   COL4A5 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A5 donor template

   Depositor

   Silvestro Conticello

   Article

   Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

   Insert

   mCherry-eGFP
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2.2-h7SKgRNA5(AAVS1)-hU6gRNA5(BbsI)-PGKpuroBFP-W

   Plasmid

   #200502

   Purpose

   Lentiviral vector expressing gRNA targeting human AAVS1

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   AAVS1 (AAVS1 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_Donor/Reporter-COL4A3

   Plasmid

   #130279

   Purpose

   COL4A3 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A3 donor template

   Depositor

   Silvestro Conticello

   Article

   Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

   Insert

   mCherry-eGFP
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV_COL4A3_self-cleavingCas9

   Plasmid

   #130281

   Purpose

   Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A3 sgRNA targets to induce its self-cleaving and inactivation

   Depositor

   Silvestro Conticello

   Article

   Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

   Insert

   spCas9
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV_COL4A5_self-cleavingCas9

    Plasmid

    #130282

    Purpose

    Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A5 sgRNA targets to induce its self-cleaving and inactivation

    Depositor

    Silvestro Conticello

    Article

    Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

    Insert

    spCas9
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C

    Plasmid

    #154196

    Purpose

    Lentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    sgRNA-BC1-A, sgRNA-BC1-C
    

    Use

    Lentiviral; Catch barcode activation

    Expression

    Mammalian

    Promoter

    hU6 and mU6

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C

    Plasmid

    #154197

    Purpose

    Lentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    sgRNA-BC2-A, sgRNA-BC2-C
    

    Use

    Lentiviral; Catch barcode activation

    Expression

    Mammalian

    Promoter

    hU6 and mU6

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Human sgRNA library Brunello in lentiGuide-Puro

    Pooled Library

    #73178

    Purpose

    Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Has Service

    Lentiviral Prep

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

14. Mouse sgRNA library Brie in lentiGuide-Puro

    Pooled Library

    #73633

    Purpose

    Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Has Service

    Lentiviral Prep

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

15. CaTCH BC1

    Plasmid

    #154195

    Purpose

    Lentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    CaTCH BC1
    

    Use

    Lentiviral; Catch barcode labeling

    Expression

    Mammalian

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. CaTCH_empty-backbone

    Plasmid

    #157746

    Purpose

    Lentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequences

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Type

    Empty backbone

    Use

    Lentiviral; Catch barcode cloning

    Expression

    Mammalian

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDECKO_GFP

    Plasmid

    #141210

    Purpose

    empty GFP vector for cloning sgRNA or pgRNAs

    Depositor

    Rory Johnson

    Article

    Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFUGW-shRIIα-RIIαWT-IRES-EGFP

    Plasmid

    #183456

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with wild-type RIIα

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFUGW-shRIIα-RIIαS97A-IRES-EGFP

    Plasmid

    #183457

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFUGW-shRIIα-RIIαS97E-IRES-EGFP

    Plasmid

    #183458

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge