We narrowed to 4,240 results for: pes

1. pMP71-tCD19-LLO-SIINFEKL

   Plasmid

   #174598

   Purpose

   expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the LLO190 and SIINFEKL epitopes

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   membrane bound CD19, LLO antigen, SIINFEKL (ovalbumin) antigen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ

   Plasmid

   #192380

   Purpose

   To test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCfB3020(gRNA X-2)

   Plasmid

   #73282

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCfB3042(gRNA X-4)

   Plasmid

   #73284

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCfB3050(gRNA XII-5)

   Plasmid

   #73292

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCfB3041(gRNA X-3)

   Plasmid

   #73283

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-3

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCfB3047(gRNA XII-1)

   Plasmid

   #73289

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-1

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCfB3046(gRNA XI-5)

   Plasmid

   #73288

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-5

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCfB3044(gRNA XI-2)

   Plasmid

   #73286

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-2

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCfB3048(gRNA XII-2)

    Plasmid

    #73290

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-2

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJK2067

    Plasmid

    #171845

    Purpose

    C. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)

    Depositor

    Judith Kimble

    Article

    Aoki et al Nat Commun. 2021 Feb 12;12(1):996. doi:

    Insert

    his-58, eGFP, mCherry
    

    Use

    Tags

    N-terminal Tag OLLAS, V5 and C-terminal Tag PEST…

    Expression

    Worm

    Mutation

    boxB wild type and hairpin mutant

    Promoter

    mex-5 promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCfB3043(gRNA XI-1)

    Plasmid

    #73285

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-1

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCfB3049(gRNA XII-4)

    Plasmid

    #73291

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-4

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSLQ1371-sgYAP-2

    Plasmid

    #121423

    Purpose

    sgYAP-2 sequence: GAGATGACTTCCTGAACAGTG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

    Depositor

    Ovijit Chaudhuri, Stanley Qi

    Article

    Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

    Insert

    sgYAP-2
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOAD eIF4G1 (1-341)

    Plasmid

    #196833

    Purpose

    Expesses S.cerevisiae eIF4G1 amino acids 1-341 as Gal4 activation domain fusion.

    Depositor

    Stan Fields

    Article

    Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

    Insert

    eIF4G1 (TIF4631 Budding Yeast)
    

    Use

    Tags

    Gal4

    Expression

    Yeast

    Mutation

    amino acids 1-341

    Promoter

    ADH

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LLP754_L2pV1_NOT_FRT_Rluc_1IF_lacZ

    Plasmid

    #192399

    Purpose

    Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-Rluc-FRT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LLP753_L2pV1_NOT_FRT_Rluc_0I_lacZ

    Plasmid

    #192398

    Purpose

    On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-Rluc-FRT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LLP730_L2_pV01_0I_FRT-OCS-TB-rLUC_TCTP-fLUC

    Plasmid

    #192373

    Purpose

    To test for the repressive potential of the OCS and Transcription Block (TB) fusion with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-TB-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LLP733_L2_pV01_0I_FRT-uORF-rLUC_TCTP-fLUC

    Plasmid

    #192376

    Purpose

    To test for the repressive potential of the conserved peptide uORF from AT1G36730 with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-uORF-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LLP727_L2_pV01_0I_TMV-rLUC_TCTP-fLUC

    Plasmid

    #192370

    Purpose

    To control for variation introduced to the gene circuits, Rluc expression not affected by the introduction of terminators or recombination target sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge