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Plasmid#129002PurposepTDH3-mRUBY2 cassette plasmid flanked by ConL2 and ConREDepositorInsertConL2-pTDH3-mRUBY2-tADH1-ConRE AmpR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0658
Plasmid#129019PurposepREV1-Zif268DBD-CRY2 cassette plasmid flanked by ConL1 and ConR2DepositorInsertConL1-pRNR2-SV40NLS-ZiF268DBD-CRY2-tSSA1-ConR2-ColE1-AmpR
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0625
Plasmid#129000PurposepREV1-mRUBY2 cassette plasmid flanked by ConL2 and ConREDepositorInsertConL2-pREV1-mRUBY2-tADH1-ConRE AmpR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0657
Plasmid#129018PurposepRPL18B-Zif268DBD-CRY2 cassette plasmid flanked by ConL1 and ConR2DepositorInsertConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2-tSSA1-ConR2-ColE1-AmpR
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0656
Plasmid#129017PurposepTEF1-Zif268DBD-CRY2 cassette plasmid flanked by ConL1 and ConR2DepositorInsertConL1-pTEF1-SV40NLS-ZiF268DBD-CRY2-tSSA1-ConR2-ColE1-AmpR
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0629
Plasmid#129004PurposepRNR2-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2DepositorInsertConL1-pRNR2-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0620
Plasmid#128995PurposepTEF1-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1DepositorInsertConLS_pTEF1-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0628
Plasmid#129003PurposepRNR2-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1DepositorInsertConLS_pRNR2-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEHF-donor
Plasmid#113817PurposeCRISPR donor plasmid to tag human transcription factor EHF with GFPDepositorInsertEHF homology arms flanking EGFP-IRES-Neo cassette (EHF Human)
UseCRISPRTagsExpressionMutationrs3818514, rs11032804, hg38:11:34659087:G>APromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBCL6-donor
Plasmid#112389PurposeCRISPR donor plasmid to tag human transcription factor BCL6 with GFPDepositorInsertBCL6 homology arms flanking EGFP-IRES-Neo cassette (BCL6 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pE2F5-donor
Plasmid#112351PurposeCRISPR donor plasmid to tag human transcription factor E2F5 with GFPDepositorInsertE2F5 homology arms flanking EGFP-IRES-Neo cassette (E2F5 Human)
UseCRISPRTagsExpressionMutationhg38:8:85213859:T>C, hg38:8:85213860:T>G, h…PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF445-donor
Plasmid#112332PurposeCRISPR donor plasmid to tag human transcription factor ZNF445 with GFPDepositorInsertZNF445 homology arms flanking EGFP-IRES-Neo cassette (ZNF445 Human)
UseCRISPRTagsExpressionMutationrs78585428, hg38:3:44446218:C>T, hg38:3:444466…PromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pELK3-donor
Plasmid#112322PurposeCRISPR donor plasmid to tag human transcription factor ELK3 with GFPDepositorInsertELK3 homology arms flanking EGFP-IRES-Neo cassette (ELK3 Human)
UseCRISPRTagsExpressionMutationhg38:12:96266960:A>T, rs760335647, rs6538704PromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#244
Plasmid#110927PurposeVector for expressing proteins in a subset of 9 DA/ DB cholingergic motor neurons in the C. elegans ventral nerve cordDepositorInsertsunc-129 promoter
unc-54 3' control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
P0050::rpaAD53A
Plasmid#101831Purposedrives expression of rpaA D53A variant from an rpaA-independent promoterDepositorInsertRpaA D53A with p0050 promoter (+ gentamicin resistance casette)
UseCloning vectorTagsExpressionMutationD53 mutated to APromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMST1212_BB2_E_BC_pcoxA:pyrGtrunc_AMA1_2.8
Plasmid#90286PurposeBB2 with split pyrG marker for homologous integration with linker BC (for 1 expression cassette)DepositorInsertpyrGtrunc
UseTagsExpressionBacterialMutationtruncated version of pyrG gene (sequence is from …PromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMST1213_BB2_E_CD_pcoxA:pyrGtrunc_AMA1_2.8
Plasmid#90287PurposeBB2 with split pyrG marker for homologous integration with linker CD (for 2 expression cassettes)DepositorInsertpyrGtrunc
UseTagsExpressionBacterialMutationtruncated version of pyrG gene (sequence is from …PromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMST1214_BB2_E_DE_pcoxA:pyrGtrunc_AMA1_2.8
Plasmid#90288PurposeBB2 with split pyrG marker for homologous integration with linker DE (for 3 expression cassettes)DepositorInsertpyrGtrunc
UseTagsExpressionBacterialMutationtruncated version of pyrG gene (sequence is from …PromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-10X-tRNA
Plasmid#70123PurposeGateway DONR vector with cassette for expression of 10 intronless S. cerevisiae tRNAsDepositorInsert10X-tRNA block
UseTagsExpressionMutationPromoterNone – SUP4 pol III promoter provided by insertAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a_Msm_RbpA
Plasmid#87928PurposeMycobacterium smegmatis RpbA in pET21a (ampR) used to co-express with Msm SigA for crystallization purposesDepositorInsertRbpA
UseTagsExpressionBacterialMutationPromoterT7PAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFLIP34
Plasmid#65501PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7TFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP35
Plasmid#65502PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-TFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP36
Plasmid#65504PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-Cer-cMycExpressionMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP42
Plasmid#65617PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-Cit-attR1 and attR2-mCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-cyEGFP-6XDSR
Plasmid#49865Purposefor Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsyEGFPExpressionMutationPromoterAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-cyEGFP-3XDSR
Plasmid#49863Purposefor Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsyEGFPExpressionMutationPromoterAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-c3HA-spo5DSR
Plasmid#49872Purposefor Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTags3XHAExpressionMutationPromoterAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-c3HA-6XDSR
Plasmid#49870Purposefor Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTags3XHAExpressionMutationPromoterAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-c3HA-3XDSR
Plasmid#49868Purposefor Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTags3XHAExpressionMutationPromoterAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-cyEGFP-8XDSR
Plasmid#49866Purposefor Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsyEGFPExpressionMutationPromoterAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only