We narrowed to 76,396 results for: cha
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Plasmid#206120Purposeexpression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1DepositorAvailable SinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only
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Cav2.2 HA E1658A (EIVA) pcDNA3
Plasmid#206114Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surfaceDepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 EIVA (E1707A) pcDNA3
Plasmid#206125Purposeexpression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation bindingDepositorAvailable SinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 HA EIA (E320A) pcDNA3
Plasmid#206124Purposeexpression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation bindingDepositorAvailable SinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 SIRT1-deltaExon2
Plasmid#105671PurposeMammalian expression construct of mouse SIRT1 short isoform (lacking Exon2)DepositorInsertSIRT1 (Sirt1 Mouse)
ExpressionMammalianMutationSynonymous base changes not affecting the protein…PromoterCMVAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-CMV-Flag-SREBP-1c
Plasmid#237348PurposeAdenoviral-SREBP-1c cleavage-activation reporter systemDepositorAvailable SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
MCUabbb-Flag-PLYS1
Plasmid#236787PurposeMCU-MCUB fusion in abbb pattern. Only the initial MCU has a mitochondrial targeting sequence.DepositorUseLentiviralTagsFLAGExpressionMammalianPromoterCMVAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDual
Plasmid#207883PurposeExpresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.DepositorInsertsThermosensitive fusion of I-OnuI and the L212P VMA1 intein.
Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
UseSynthetic BiologyExpressionBacterialMutationLeucine 212 changed to proline, leucine 434 in th…Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIVA (E1658A) pCAGGS
Plasmid#206082Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surfaceDepositorAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIA (E314A) pCAGGS
Plasmid#206081Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain I which reduces trafficking to the cell surfaceDepositorAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIVK (E1658K) pcDNA3
Plasmid#206066Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surfaceDepositorAvailable SinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIVK (E1658K) pCAGGS
Plasmid#206096Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surfaceDepositorAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4 TAPBPR-TM-TN6
Plasmid#178650PurposeMammalian expression of FLAG-tagged TAPBPR with MHC TM (E205K, R207E, Q209S, Q272S)DepositorInsertTAPBPR (TAPBPL Human)
TagsFLAG and Signal/leader sequence from HLA class I …ExpressionMammalianMutationE205K, R207E, Q209S, Q272S, switched transmembran…PromoterCMVAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3 SIRT1-NTerm Exon1-Exon3
Plasmid#105681PurposeBacterial Expression construct of N terminus of SIRT1: Exon1-Exon3 (deltaExon2)DepositorInsertSIRT1-NTerm: Exon1-Exon3 (Sirt1 Mouse)
TagsGSTExpressionBacterialMutationSynonymous base changes not affecting the protein…Promotertac_PromoterAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3- SIRT1-NTerm Exon1-Exon2-Exon3
Plasmid#105680PurposeBacterial Expression construct of N terminus of SIRT1: Exon1-Exon2-Exon3DepositorInsertSIRT1-NTerm: Exon1-Exon2-Exon3 (Sirt1 Mouse)
TagsGSTExpressionBacterialMutationSynonymous base changes not affecting the protein…Promotertac_PromoterAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLgw EcoDam-V5-NKX2-5Y191C
Plasmid#98598PurposeMammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloningDepositorInsertNKX2-5 (Nkx2-5 Mouse)
UseLentiviral; DamidTagsDam (DNA adenine methyltransferase) and V5ExpressionMammalianMutationY191C point mutantPromoterHeat Shock Minimal PromoterAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLgw NKX2-5Y191C-V5-EcoDam
Plasmid#98599PurposeMammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloningDepositorInsertNKX2-5 (Nkx2-5 Mouse)
UseLentiviral; DamidTagsV5 and Dam (DNA adenine methyltransferase)ExpressionMammalianMutationY191C point mutantPromoterHeat Shock Minimal PromoterAvailable SinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMiR-CIP2A-stop-mut
Plasmid#53713PurposeLuciferase reporter assay for CIP2A with mutated firefly stop codonDepositorInsertCIP2A (CIP2A Human)
UseLuciferaseMutationMutation on the stop codon of firefly luciferase …Available SinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP
Plasmid#31484DepositorInsertEF1s HA NLS Sce(opt) T2A IFP1.4
UseLentiviralTagsHA and NLSMutation"Opt" indicates the I-SceI coding seque…Available SinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)
Plasmid#31476DepositorInsertSFFV d14 GFP donor EF1s I-SceI
UseLentiviralTagsHA and NLSMutationGFP has 14 amino acids deleted from c terminusAvailable SinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV-EF1s HA.NLS.Sce(opt)
Plasmid#31479DepositorInsertSFFV/Ef1s HA NLS I-SceI
UseLentiviralTagsHA and NLSMutation"opt" indicates the coding sequence has…Available SinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX iLOV
Plasmid#26587DepositorInsertiLOV
TagsGSTExpressionBacterialAvailable SinceDec. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pM201
Plasmid#173179PurposedCas9-24xGCN4DepositorInsertdCas9-24xGCN4
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterCMVAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-Sec16L
Plasmid#15776DepositorAvailable SinceNov. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-L
Plasmid#51226Purposebi-directional expression vector for yeast, constitutively active, LEU2 selectionDepositorTypeEmpty backboneExpressionYeastPromoterGPD/ADH1Available SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NMHC II-C
Plasmid#10843DepositorAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pclbw-mitoTagRFP
Plasmid#58425PurposeMammalian expression of tagRFP targeted to the mitochondrial matrixDepositorInsertmito-tagRFP
UseRetroviralExpressionMammalianPromotercmv-bactinAvailable SinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TurboID-3MYC-kanMX6 (pFB1420)
Plasmid#126049PurposeFor C-terminal tagging with TurboID in yeast including geneticin-resistant markerDepositorInsertTurboID
UsePcr-based c-terminal taggingAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-PP7v3
Plasmid#107230PurposeExpresses 24x optimized PP7 stem loops (version 3) in bacterial cellsDepositorInsert24xPP7v3
Tags3x FLAG-tag and P2A siteExpressionBacterialAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TurboID-3MYC-natMX6 (pFB1434)
Plasmid#126050PurposeFor C-terminal tagging with TurboID in yeast including nourseothricin-resistant markerDepositorInsertTurboID
UsePcr-based c-terminal taggingAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-mRuby2-FRET-10
Plasmid#58179PurposeLocalization: Cytosolic FRET, Excitation: , Emission:DepositorTypeEmpty backboneTagsmNeonGreen and mRuby2ExpressionMammalianAvailable SinceMarch 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
5'UTR CGG 99x FMR1-EGFP
Plasmid#63091PurposeContains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with EGFP.DepositorInsertexpanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable SinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pFLAG ARMS
Plasmid#24086DepositorAvailable SinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
hUbc13
Plasmid#51131Purposehuman ubc13, E2 for synthesis k63 linked ubiquitin chainDepositorAvailable SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
VV073: 1xCox8 - GCaMP5G in fubi
Plasmid#58509PurposeExpresses the calcium sensor GCaMP5G in the mitochondriaDepositorInsert1xCox8 - GCaMP5G
UseLentiviralExpressionMammalianPromotera-CamKIIAvailable SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-IRAlus-Nicn1
Plasmid#92345PurposePlasmid for expression of GFP gene with a Nicn1 gene derived IR Alu element containing 3'UTR.DepositorInsertNicn1 gene derived IR Alu element containing 3'UTR (NICN1 Human)
ExpressionMammalianPromoterCMVAvailable SinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-rev-ChR2-tdtomato
Plasmid#18917PurposeCre-dependent viral expression of ChR2-tdTomato for neuronal excitationDepositorInsertChannelrhodopsin 2-tdtomato
UseAAV and Cre/Lox; Adeno-associated virusTagstdtomatoPromoterCAGAvailable SinceFeb. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-lacO-His-sfGFP
Plasmid#180754PurposepET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
Tagsstrep tagExpressionBacterialMutationG140C- Please see depositor commentsAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
N-GFP-Dcp2
Plasmid#163585Purposeexpress N-terminal GFP tagged Dcp2 FL at endogenous level in S.cerevisiaeDepositorAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only