We narrowed to 7,695 results for: ALP

1. pLVX MLKL(wt)--2A-mCherry-puro

   Plasmid

   #231974

   Purpose

   Tet inducible expression of DmrB-Mlkl with mCherry to induce WT Mlkl expression

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   MLKL WT construct with bi-cistronic mCherry (Mlkl Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgTAZ-R-3

   Plasmid

   #229450

   Purpose

   knockout of WWTR1 (TAZ)

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   sgTAZ-R-3 (WWTR1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6;bU6;EFS

   Available Since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX462-hPRKAA2-gRNA_B

   Plasmid

   #74377

   Purpose

   gRNA_B to knockout human AMPK alpha 2 using Cas9n

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   human AMPK alpha 2 exon1 gRNA (PRKAA2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. JAM3-bio-His

   Plasmid

   #51807

   Purpose

   Expresses full-length Junctional adhesion molecule C precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   JAM3 (JAM3 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221_SLC51A

   Plasmid

   #132240

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC51A (SLC51A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 21, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. SSN-GFP

   Plasmid

   #163663

   Purpose

   Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail and TMD from ST6GAL1, and luminal domain from TNF in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   This chimera contains the cytosolic tail from ST,…

   Promoter

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. NSN-GFP

   Plasmid

   #163665

   Purpose

   Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from TNF, TMD from ST6GAL1 and luminal domain from TNF in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   This chimera contains the cytosolic tail from TNF…

   Promoter

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACEBac1-gamma-TuSC

   Plasmid

   #178079

   Purpose

   Insect cell expression of gamma-TuSC

   Depositor

   Tarun Kapoor

   Article

   Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

   Inserts

   gamma-tubulin (TUBG1 Human)
   GCP2 (TUBGCP2 Human)
   GCP3 (TUBGCP3 Human)
   

   Use

   Tags

   TEV-His6

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. LZRS-BRaf-ERTm

   Plasmid

   #24949

   Depositor

   Paul Khavari

   Article

   Dajee et al Oncogene. 2002 Feb 28;21(10):1527-38

   Insert

   BRaf-ER (Esr1, BRAF Human, Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   V600E in BRAF

   Promoter

   Available Since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR-Flag-IKKbeta-KM

    Plasmid

    #15466

    Depositor

    Hiroyasu Nakano

    Article

    Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

    Insert

    IkB kinase beta-KM (Ikbkb Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Lysine at 44 to Alanine

    Promoter

    Available Since

    Oct. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

11. LAMP2-bio-His

    Plasmid

    #51861

    Purpose

    Expresses full-length Lysosome-associated membrane glycoprotein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    LAMP2 (LAMP2 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMCSF-R(mB)-luc

    Plasmid

    #12422

    Depositor

    Dong-Er Zhang

    Article

    Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

    Insert

    M-CSF receptor promoter fragment (CSF1R Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    AML1 binding site mutation (created a KpnI site i…

    Promoter

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

13. ARMET-bio-His

    Plasmid

    #52026

    Purpose

    Expresses full-length Mesencephalic astrocyte-derived neurotrophic factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ARMET (MANF Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. LRRC8b-His-bio

    Plasmid

    #53362

    Purpose

    Expresses full-length Leucine rich repeat containing 8 family, member B ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    LRRC8b (LRRC8B Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. SITS-mCherry-Bin1b

    Plasmid

    #176025

    Purpose

    Leishmania cell free expression of zebrafish mCherry-Bin1b. Parton lab clone GSU

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1b (bin1b Zebrafish)
    

    Use

    Leishmania cell free expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAcBac1-Mb-Fluoro-Phe B5

    Plasmid

    #197576

    Purpose

    Expression of Methanosarcina barkeri (Mb) Fluoro-Phe B5 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10

    Inserts

    Mb Pyl Fluoro-Phe B5 synthetase
    Pyl-tRNA (4 copies)
    

    Use

    Tags

    Nuclear export sequence + FLAG

    Expression

    Mammalian

    Mutation

    N311G, C313A

    Promoter

    CMV and U6/H1

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21a-Med25-VBD-6His

    Plasmid

    #64773

    Purpose

    Bacterial expression of His-tagged Med25-VBD

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    Med25-VBD (MED25 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    insert is 394-543 only

    Promoter

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

18. CD40-bio-His

    Plasmid

    #51648

    Purpose

    Expresses full-length Tumor necrosis factor receptor superfamily member CD40 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    CD40 molecule (CD40 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EPHB1-bio-His

    Plasmid

    #51750

    Purpose

    Expresses full-length Ephrin type-B receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    EPHB1 (EPHB1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQCXIP mScarlet-MOSPD2 W201E

    Plasmid

    #186477

    Purpose

    Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to mScarlet in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Retroviral

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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