We narrowed to 31,166 results for: ica
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Plasmid#193792PurposeCassette 1: Expresses frame-shifted CI under PLLacO-1 promoter, Cassette 2: Expresses frame-shifted CI under PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertTwo copies of frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLLacO-1 and PLTetO-1Available sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV/CaMKIIa::iLMO2
Plasmid#129481PurposeAAV2 transfer plasmid for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical, under control of the glutamatergic-selective CaMKIIa promoterDepositorInsertiLMO2
UseAAVTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS238D1::sptse5-CT
Plasmid#192959PurposeFor expression of Tse5-CT (1169-1317) in P. putida with the PelB signal peptide fused at the N-terminus in biological function studiesDepositorInsertsptse5-CT
UseTagsPelB signal peptideExpressionBacterialMutationencodes for tse5 (1169-1317)PromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE070
Plasmid#176827PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert709
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE072
Plasmid#176829PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert711
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
eDrMet
Plasmid#183999PurposeOptically controlled eDrcMet expression in mammalian cellsDepositorInserteDrMet (MET Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
HEpl016
Plasmid#179991PurposepCBh_3xFLAG_NLS_SiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized SiCas12i driven by CBh promoter and tagmCherry driven by CMV promoterDepositorInsertSiCas12i
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2 SS/R
Plasmid#166976PurposeExpresses FBF-2 with SS/R mutations in repeat 5 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
UseTagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575…PromoterT7Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2 AQ/Y
Plasmid#166975PurposeExpresses FBF-2 with AQ/Y mutations in repeat 5 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
UseTagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575…PromoterT7Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCu-His6-Atg13[571–700]-Ss(424)
Plasmid#166852PurposeExpresses 6xHis tagged ATG13 [571–700] fragment with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast.DepositorInsertAtg13[571–700] (ATG13 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterpCuAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 SEC66-VAC8-GFP
Plasmid#166840PurposeExpresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorUseTagsGFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
FUG-T2A-H93R
Plasmid#140739PurposeLentiviral vector to overexpress the autism-associated PTEN mutation H93R and GFP as two separate proteinsDepositorInsertGFP-T2A-PTENH93R (PTEN Human)
UseLentiviralTagsnoneExpressionMammalianMutationH93RPromoterhAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUG-PtenN276S
Plasmid#140737PurposeLentiviral vector to overexpress the PTEN mutation N276S as a GFP-PTEN fusion proteinDepositorInsertPTEN – phosphatase and tensin homolog (PTEN Human)
UseLentiviralTagsGFPExpressionMammalianMutationN276SPromoterhUbiCAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUG-PtenW274L
Plasmid#140736PurposeLentiviral vector to overexpress the autism-associated PTEN mutation W274L as a GFP-PTEN fusion proteinDepositorInsertGFP-PTEN (PTEN Human)
UseLentiviralTagsGFPExpressionMammalianMutationW274LPromoterhUbiCAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUG-PtenD252G
Plasmid#140735PurposeLentiviral vector to overexpress the autism-associated PTEN mutation D252G as a GFP-PTEN fusion proteinDepositorInsertGFP-PTEN (PTEN Human)
UseLentiviralTagsGFPExpressionMammalianMutationD252GPromoterhUbiCAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUG-PtenH93R
Plasmid#140733PurposeLentiviral vector to overexpress the autism-associated PTEN mutation H93R as a GFP-PTEN fusion proteinDepositorInsertGFP-PTEN (PTEN Human)
UseLentiviralTagsGFPExpressionMammalianMutationH93RPromoterhUbiCAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSHDY*in_PpetE:BCD2:MrBBS:Strep
Plasmid#133973PurposeHeterologous, copper-inducible expression of (-)-α- bisabolol synthase from Matricaria recutita (MrBBS) in Synechocystis sp. PCC 6803. The MrBBS CDS is fused to a C-termial StrepII-tag.DepositorInsertMrBBS:StrepII
UseSynthetic BiologyTagsStrepIIExpressionBacterialMutationinserted gene is codon optimized for Synechocyst…PromoterPpetE:BCD2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 PUM FL
Plasmid#112997PurposeFor protein expression and purification of Drosophila pumilio (amino acids 566-1090)DepositorInsertpumilio (pum Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_FNBP1-1/1
Plasmid#91552PurposeProtein expression and purification of human SH3 domain construct FNBP1-1/1DepositorInsertFNBP1-1/1 (FNBP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ABL2-1/1
Plasmid#91533PurposeProtein expression and purification of human SH3 domain construct ABL2-1/1DepositorInsertABL2-1/1 (ABL2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only