We narrowed to 8,468 results for: sms

1. pENN496_miniCRISPRcharm_dSaCas9

   Plasmid

   #220837

   Purpose

   mini CRISPRcharm expression in mammalian cells

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   mini CRISPRcharm
   

   Use

   CRISPR

   Tags

   P2A-TagBFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EFS

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRE-Lepr_FL-IRES-eGFP

   Plasmid

   #196635

   Purpose

   Doxycycline inducible expression of full length mouse Lepr with eGFP marker

   Depositor

   Elaine Fuchs

   Article

   Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

   Insert

   Lepr (Lepr Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT/TO-eGFP-53BP1

   Plasmid

   #60813

   Purpose

   mammalian expression vector

   Depositor

   Daniel Durocher

   Article

   Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

   Insert

   53BP1 (TP53BP1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   Dec. 9, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. AbVec2.0-mIgkc

   Plasmid

   #127157

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), kappa constant region

   Depositor

   Hedda Wardemann

   Article

   Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

   Insert

   Igkc (Igkc Mouse)
   

   Expression

   Mammalian

   Promoter

   hCMV IE1 gene promoter

   Available Since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV_TRET_hNgn2_UBC_Puro

   Plasmid

   #61474

   Purpose

   3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection marker

   Depositor

   Ron Weiss

   Article

   Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

   Inserts

   Ngn2 (NEUROG2 Human)
   Puro
   

   Use

   Lentiviral

   Available Since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-3xFLAG-bIV spectrin (repeats 13-15)

   Plasmid

   #213728

   Purpose

   Mammalian cell expression vector for overexpression of bIV spectrin (repeats 13-15) in NIH/3T3 cells.

   Depositor

   Chaolin Zhang

   Article

   Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

   Insert

   bIV spectrin (repeats 13-15) (Sptbn4 Mouse)
   

   Expression

   Mammalian

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX6P1-3xMBT

   Plasmid

   #46987

   Purpose

   Inducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coli

   Depositor

   Or Gozani

   Article

   Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:

   Insert

   Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)
   

   Tags

   GST and PreScission cleavage site

   Expression

   Bacterial

   Mutation

   contains amino acids 190–530 of accession NP_0562…

   Promoter

   tac

   Available Since

   Sept. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR

   Plasmid

   #171594

   Purpose

   Strong human ACE2 abundance from Bxb1 landing pad

   Depositor

   Kenneth Matreyek

   Article

   Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

   Inserts

   ACE2 (ACE2 Human)
   mCherry
   H2A
   Pac
   

   Use

   Synthetic Biology; Promoterless bxb1 dna recombin…

   Expression

   Mammalian

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOPINK-OTUD3 (OTU, aa 52-209)

   Plasmid

   #61412

   Purpose

   Expresses human OTUD3 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD3 (OTUD3 Human)
   

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-51 and aa 210-398.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET26b_AT118-H-V5-His

    Plasmid

    #222850

    Purpose

    E. coli periplasmic expression plasmid encoding Nb. AT118-A, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-H
    

    Tags

    V5-His

    Expression

    Bacterial

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-mDyn3 pcDNA3

    Plasmid

    #36265

    Depositor

    Pietro De Camilli

    Article

    Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

    Insert

    Dynamin 3 (Dnm3 Mouse)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2012

    Availability

    Academic Institutions and Nonprofits only

12. lenti-EF1a-dCas9-VP64-Puro

    Plasmid

    #99371

    Purpose

    3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    dCas9-VP64-T2A-Puro
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

    Plasmid

    #99278

    Purpose

    Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance

    Depositor

    Jordan Miller

    Article

    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

    Inserts

    H2B-mVenus-IRES-mCherry
    Puro
    

    Use

    Lentiviral

    Tags

    H2B, IRES, PGK, mCherry, and mVenus

    Promoter

    EF1 alpha-human and PGK-mouse

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Sniper2L

    Plasmid

    #193856

    Purpose

    Expresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/

    Insert

    Sniper2L
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    E1007L

    Promoter

    EFS

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cherry-Fibrillarin-C1

    Plasmid

    #134539

    Purpose

    Expresses Cherry-Fibrillarin fusion protein

    Depositor

    Susan Janicki

    Article

    Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

    Insert

    Fibrillarin (FBL Human)
    

    Tags

    Cherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMP71-super-IL-2

    Plasmid

    #174599

    Purpose

    Expresses extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) developed by the Garcia Lab

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    extracellular domain of huIL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F)
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-HA AKT1

    Plasmid

    #78778

    Purpose

    To overexpress AKT1 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

    Insert

    AKT1 (AKT1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. AbVec2.0-mIghg2b

    Plasmid

    #127155

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2b isotype

    Depositor

    Hedda Wardemann

    Article

    Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

    Insert

    Ighg2b (Ighg2b Mouse)
    

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-eNme2-T.1-ABE8e

    Plasmid

    #185668

    Purpose

    Expresses eNme2-T.1-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-neNme2-T.2
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOPINK-OTUD6B (full-length, aa 1-293)

    Plasmid

    #61418

    Purpose

    Expresses human OTUD6B (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6B (OTUD6B Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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