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Cofilin1_pLVX-EF1-Cry2-mCherry

Plasmid

#64370

Purpose

lentiviral expression of Cofilin1 and Cry2-mCherry

Depositor

Matthieu Piel

Insert

Cofilin1 (CFL1 Human)

Use

Lentiviral

Tags

Cry2-mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
EZRA-c004

Plasmid

#175461

Purpose

Protein expression in bacterial cells. FERM domain, M1-Q322.

Depositor

Opher Gileadi

Insert

EZR (EZR Human)

Tags

His6-TEV

Expression

Bacterial

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCHTV86.1-2.2

Plasmid

#115544

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3

Expression

Yeast

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCHKU17.1-2.2

Plasmid

#115530

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3

Expression

Yeast

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU62.2-2.2

Plasmid

#110676

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3

Expression

Yeast

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU65.2-2.2

Plasmid

#110678

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3

Expression

Yeast

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCHKU42.2-2.2

Plasmid

#110673

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3

Expression

Yeast

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU72.2-2.2

Plasmid

#110687

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3

Expression

Yeast

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf200

Plasmid

#12888

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf200

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
EGFP-Ubiquitin

Plasmid

#187910

Purpose

Mammalian cell expression of EGFP-tagged Ubiquitin

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

UBB (UBB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_Mu

Plasmid

#177578

Purpose

expressing SARS-CoV-2 B.1.621 (Mu) spike protein for pseudovirus production

Depositor

David Nemazee

Article

Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

Insert

Spike (Mu variant) (S SARS-CoV-2)

Use

Retroviral

Expression

Mammalian

Mutation

T95I, Y144T, Y145S, R346K, E484K, N501Y, D614G, …

Promoter

CMV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-s-IRESWT-Gbetagamma ONE-GO

Plasmid

#204340

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-s/Gbetagamma ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS SARS-CoV-2 BQ.1.1 Spike

Plasmid

#193710

Purpose

Expresses codon-optimized full length SARS-CoV-2 BQ.1.1 Spike

Depositor

Marceline Côté

Article

Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

Insert

SARS-CoV-2 BQ.1.1 Spike (S )

Expression

Mammalian

Mutation

Contains the following mutations: T19I, LPPA24S, …

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

Nov. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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GST scFv [N100/13]

Plasmid

#190521

Purpose

Mammalian Expression of GST scFV. Derived from hybridoma N100/13.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GST (Schistosoma japonicum) recombinant scFV

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_HSPA5-Halo

Plasmid

#175326

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HSPA5 (HSPA5 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_Lambda

Plasmid

#177579

Purpose

expressing SARS-CoV-2 C.37 (lambda) spike protein for pseudovirus production

Depositor

David Nemazee

Article

Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

Insert

Spike (Lambda variant) (S SARS-CoV-2)

Use

Retroviral

Expression

Mammalian

Mutation

75-76VI, 246-253N, L452Q, F490S, D614G, T859N, la…

Promoter

CMV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Salmonella MTAN

Plasmid

#64041

Purpose

Expression of S. enterica MTAN in E. coli

Depositor

Vern Schramm

Article

Hemeon et al Anal Chem. 2011 Jun 15;83(12):4996-5004.

Insert

Methylthioadenosine nucleosidase

Tags

Histidine tag

Expression

Bacterial

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
pαH-S-GSAS-B.1.1.7

Plasmid

#171747

Purpose

Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-B.1.1.7 variant (UK)

Depositor

Priyamvada Acharya

Article

Gobeil et al Science. 2021 Jun 24. pii: science.abi62

Insert

Spike (S-GSAS-B.1.1.7 variant) (S Severe acute respiratory syndrome coronavirus 2)

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available Since

July 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 BA.2.75 Spike

Plasmid

#190012

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.2.75 Spike

Depositor

Marceline Côté

Article

Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

Insert

SARS-CoV-2 BA.2.75 Spike (S )

Expression

Mammalian

Mutation

Contains the BA.2 mutations (T19I, LPPA24S, G142D…

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

Sept. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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QPRTA-c002

Plasmid

#205115

Purpose

QPRT protein expression

Depositor

Paul Brennan

Insert

QPRT (QPRT Human)

Tags

TEV-6His

Expression

Insect

Mutation

WT

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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