We narrowed to 11,287 results for: aga

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pYNL-N1_3xNLS

Plasmid

#65672

Purpose

Expresses NLS-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

three copies of SV40 nuclear localization signal

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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PCDNA3-N3HA-TAF12-HFD(50-130)

Plasmid

#105613

Purpose

transient express TAF12 HFD with 3* HA tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 HFD amino acid 50-130 with HA tag (Taf12 Mouse)

Tags

3*HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-TAF4(827-909)

Plasmid

#105614

Purpose

transient express TAF4 HFD in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF4 HFD amino acid 827-909 (Taf4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAP2042-1

Plasmid

#105244

Purpose

mammalian eGFP with TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

eGFP with extra-sequence (TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker::eGFP)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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M26V DJ-1 pET15b

Plasmid

#60688

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26V DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26V

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pLKO.1 shPIK3CD.v2 puro

Plasmid

#58707

Purpose

Lentiviral shRNA vector for knockdown of human PIK3CD

Depositor

Kevin Janes

Article

Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

Insert

shPIK3CD (PIK3CD Human)

Use

Lentiviral

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only
pYNL-N1_Mito

Plasmid

#65669

Purpose

Expresses Mito-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

cytochrome c oxidase subunit VIII

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly-tj

Plasmid

#86386

Purpose

Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Insert

D. melanogaster tj enhancer

Use

Stap-seq screening vector

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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hSTARR-seq_SULT1C vector

Plasmid

#99294

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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hSTARR-seq_TTF2 vector

Plasmid

#99293

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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spa-GCaMP6m

Plasmid

#67557

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6m

Tags

HIS-tag

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pONL-N1_zyxin

Plasmid

#65683

Purpose

Expresses Zyxin-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

zyxin (Zyx Mouse)

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2015

Availability

Academic Institutions and Nonprofits only
pONL-N1_3xNLS

Plasmid

#65674

Purpose

Expresses NLS-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

three copies of SV40 nuclear localization signal

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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sspa-GCaMP6m

Plasmid

#67560

Purpose

short photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

sspa-GCaMP6m

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pONL-N1_TUBB5

Plasmid

#65680

Purpose

Expresses beta-tubulin-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

beta-tubulin class V

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pHRIG-S6K

Plasmid

#59446

Purpose

lentiviral expression of S6K and EGFP

Depositor

Heng Zhao

Article

Xie et al Stroke. 2014 Jul 10. pii: STROKEAHA.114.

Insert

S6K (Rps6kb1 Rat)

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mammalian

Mutation

S6K starts at aa24 and contains 7 extra amino aci…

Promoter

CMV

Available Since

Oct. 28, 2014

Availability

Academic Institutions and Nonprofits only
D10 pAcGP67a

Plasmid

#41812

Depositor

Chris Garcia

Article

Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

Insert

D10 Super-2 (IL2 Human)

Tags

8x His

Expression

Insect

Promoter

Polyhedron

Available Since

Jan. 8, 2013

Availability

Academic Institutions and Nonprofits only
pGEX RalB G23V ΔC

Plasmid

#50995

Purpose

Expresses RalB G23V ΔC in bacterial cells

Depositor

Anna Sablina

Article

Simicek et al Nat Cell Biol. 2013 Oct;15(10):1220-30.

Insert

RALB (RALB Human)

Tags

GST

Expression

Bacterial

Mutation

G23V ΔC

Promoter

tac_promoter

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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MET FLAG46QDProCFPp303

Plasmid

#15589

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 46Q Delta Pro (HTT Human)

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
GAL HA25Q+ProCFPp303

Plasmid

#15570

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q + Pro (HTT Human)

Tags

CFP and HA

Expression

Yeast

Mutation

Proline-rich region present

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only