We narrowed to 14,304 results for: TIM;
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Plasmid#209446Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning siteDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyPromoterPtipA; sgRNA expression from ermE* promoterAvailable SinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-3xrGBD
Plasmid#176108PurposeVisualization of active RhoDepositorAvailable SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPS0382
Plasmid#133232PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter PsNifH (pOGG043), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-mRFP-pcDNA3.1-
Plasmid#52510Purposeexpresses mRFP in mammalian cells with FLAG-HA tag at N-terminusDepositorInsertmRFP
TagsFLAG-HAExpressionMammalianMutationengineered variant of tetrameric red fluorescent …PromoterCMVAvailable SinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mCherry2-L
Plasmid#104003PurposePlasmid encoding mCherry2-LDepositorInsertmCherry2-L
UseLow copyExpressionBacterialMutationMutations relative to DsRed (MSKGEE NNLA IIKEF… V…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-VSVG
Plasmid#80606PurposeMammalian expression vector that expresses a VSV-G protein with a FLAG tag near the N-terminus of the proteinDepositorInsertVSV-G
TagsFLAGExpressionMammalianPromoterCMVAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-HA-Suv4-20h1
Plasmid#86689PurposeFor transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'DepositorAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-LiMETER-v1
Plasmid#113933PurposeExpress an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFPDepositorInsertLOV2 and Rit polybasic domain (PB)
TagsGFPExpressionMammalianPromoterCMVAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
CD40L-MIEG-hCD4 (hCD4-CD40L)
Plasmid#40355DepositorAvailable SinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pclbw-eGFPomp25
Plasmid#58424PurposeMammalian expression of GFP targeted to the mitochondrial outer membraneDepositorInserteGFP-omp25 (Synj2bp Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationcontains aa's 109-145 of mouse omp25PromoterCMV/b-actinAvailable SinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha11_SulR
Plasmid#186424Purposetranscriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter; suitable for quintuple assemblyDepositorInsertSulR
UseSynthetic Biology; Binary vector for escherichia …ExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-QUAS5x:GFPNLS-SV40pA
Plasmid#155122PurposeTol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertQUAS5x:GFPNLS
UseZebrafish expressionPromoterQUAS5x-E1bAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB2-neo-TET
Plasmid#51287Purposeexpresses the rodent SINE, B2, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassetteDepositorInsertsine B2
TagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianPromoterenhancer from 7SL geneAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMY-MCS-P2A-eGFP
Plasmid#163356PurposeRetroviral backbone for in-frame cloning of a gene of interest and a P2A separated GFP markerDepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
SWEET11:SWEET11-2A-GFP-GUS
Plasmid#172484PurposeTranslational fusion of SWEET11 with a P2A self cleaving peptide sequenceDepositorAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMY-MCS-P2A-CD90.2
Plasmid#163355PurposeRetroviral backbone for in-frame cloning of a gene of interest and a P2A separated CD90.2 surface markerDepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP gRNA (BRDN0000562805)
Plasmid#80035Purpose3rd generation lentiviral gRNA plasmid targeting EGFPDepositorInsertEGFP
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA16_Efa-NLS(SV40) (KAC459)
Plasmid#134337PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Efa with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA16_Efa
TagsNLS(SV40)ExpressionMammalianPromoterCMV and T7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI
Plasmid#226011PurposeCasRx guide RNA cloning backbone (lenti)DepositorTypeEmpty backboneUseLentiviralAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIV301
Plasmid#204969PurposeAMA1 plasmid with Aspergillus optimized Mad7 and ble (bleomycin) resistance markerDepositorInsertsMad7
ble (bleomycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only