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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,362 results for: Coli

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Showing: 8241 - 8280 of 10362 results

  1. pHS1232

    Plasmid
    #205969
    Purpose
    MWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promoters
    Depositor
    Feng Zhang
    Article
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Insert
    Cas5-HNH proteins and CRISPR array
    Expression
    Bacterial
    Promoter
    Lac (proteins), pJ23119 (crRNA)
    Available Since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205969-plasmid-map-sequence-id-416250

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  2. pET26b(+)-ATI_QconCAT_1

    Plasmid
    #163957
    Purpose
    Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.
    Depositor
    Stefan Tenzer
    Article
    Sielaff et al J Proteome Res. 2021 Jan 28. doi: 10.102
    Insert
    ATI QconCAT
    Tags
    polyhistidine tag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    163957-plasmid-map-sequence-id-321834

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  3. pQCascade-IS186

    Plasmid
    #140627
    Purpose
    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS186. The crRNA-IS186 targets IS186 loci in Escherchia coli.
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS186
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Available Since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    140627-plasmid-map-sequence-id-310685

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  5. pUDP012

    Plasmid
    #101167
    Purpose
    E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    ScTDH3
    Available Since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101167-plasmid-map-sequence-id-199268

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  7. pSW002-Pc-TorT(sp)-mNeonGreen

    Plasmid
    #205021
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    TorT-mNeonGreen
    Expression
    Bacterial
    Mutation
    mNeonGreen is codon optimized for expression in P…
    Promoter
    Pc
    Available Since
    Sept. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    205021-plasmid-map-sequence-id-407999

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  17. pQCascade-IS3

    Plasmid
    #140626
    Purpose
    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS3. The crRNA-IS3 targets IS3 loci in Escherchia coli.
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS3
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Available Since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140626-plasmid-map-sequence-id-305994

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  23. pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM

    Plasmid
    #107163
    Purpose
    Expresses specific cyclic-ADP-ribose (cADPR) phosphohydrolase in Escherichia coli as a GST fusion protein that can be cut with PreScission(TM) protease to yield active enzyme
    Depositor
    Jose Carlos Cameselle
    Article
    Ribeiro et al Sci Rep. 2018 Jan 18;8(1):1036. doi: 10.
    Insert
    F37A+L196F+V252A+C253G-hADPRM GST-mutant cADPR phosphohydrolase gene
    Tags
    GST
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107163-plasmid-map-sequence-id-204806

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  25. pAR69_STRI_m(KS_MAT)_H8_pET22b

    Plasmid
    #122849
    Purpose
    Expresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.
    Depositor
    Martin Grininger
    Article
    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi
    Insert
    m(KS_MAT) (Fasn Mouse)
    Tags
    His8 tag and Twin-Strep tag
    Expression
    Bacterial
    Mutation
    wildtype
    Promoter
    T7 promoter
    Available Since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122849-plasmid-map-sequence-id-238542

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  26. pQCascade-IS2

    Plasmid
    #140625
    Purpose
    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS2. The crRNA-IS2 targets IS2 loci in Escherchia coli.
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS2
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Available Since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140625-plasmid-map-sequence-id-305996

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  38. p-OiRS3GG

    Plasmid
    #98589
    Purpose
    Expresses default target RNA (gI intron) and pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).
    Depositor
    Lydia Contreras
    Article
    Vazquez-Anderson et al Nucleic Acids Res. 2017 May 19;45(9):552
    Inserts
    group I intron
    PheS cassette to be selectively replaced by unique asRNA probes
    Expression
    Bacterial
    Promoter
    pBAD and pLtetO
    Available Since
    Sept. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    98589-plasmid-map-sequence-id-194130

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Showing: 8241 - 8280 of 10362 results