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pQTEV-STX10

Plasmid

#31572

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

syntaxin 10 (STX10 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pQTEV-MTPN

Plasmid

#34829

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

myotrophin (MTPN Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pLN397 (FuGW-S(E2F1)p-Sponge7)

Plasmid

#105181

Purpose

Module 2 - synthetic promoter E2F1 drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(E2F1)p-Sponge7

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCSN074

Plasmid

#166731

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Article

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-NLS

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-2x

Plasmid

#172282

Purpose

Expressing EGFP mRNA fused with 2 tandem repeats of a 50-base sequence

Depositor

Antony K Chen

Article

Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

Insert

EGFP-N1-2x

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-4x

Plasmid

#172283

Purpose

Expressing EGFP mRNA fused with 4 tandem repeats of a 50-base sequence

Depositor

Antony K Chen

Article

Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

Insert

EGFP-N1-4x

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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h-alpha-ENaC-2

Plasmid

#83431

Purpose

full length human alpha ENaC-2 with flag tag

Depositor

Christie Thomas

Article

Thomas et al Am J Physiol. 1998 May;274(5 Pt 1):C1312

Insert

human alpha ENaC isoform 2 (SCNN1A Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Rab4a-7

Plasmid

#55443

Purpose

Localization: Ras GTPase, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Rab4a (RAB4A Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pMX155

Plasmid

#29481

Depositor

Linda Wordeman

Article

Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Insert

Kif2A-alpha (Kif2a Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

E140K

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-9D

Plasmid

#114029

Purpose

Inducible expression of eGFP-tagged HEC1-9D in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1 - 9D (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 9D

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLBC0010

Plasmid

#172774

Purpose

Level 0 module containing NT1_CTPSSU

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Gene sequence for Rubisco small subunit (SSU) chloroplast transit peptide (CTP) of Arabidopsis thaliana

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0011

Plasmid

#172775

Purpose

Level 0 module containing NT2_CTPSSU

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Gene sequence for Rubisco small subunit (SSU) chloroplast transit peptide (CTP) of Arabidopsis thaliana

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0060

Plasmid

#172783

Purpose

Level 0 module containing NT2_cYFP3XHA

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS C-terminal eYFP (175-238) 3XHA tag

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0061

Plasmid

#172784

Purpose

Level 0 module containing CT_cYFP3XHA

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS C-terminal eYFP (175-238) 3XHA tag

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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mOrange2-Endo-14

Plasmid

#57955

Purpose

Localization: Endosomes, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Endo (RHOB Human)

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pETM6-Pc4CL-PhCHS-MsCHI

Plasmid

#73396

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Pc4CL
PhCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-Vv4CL-PhCHS-CmCHI

Plasmid

#73401

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Vv4CL
PhCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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IRE-TPI-wildtype-TREAT

Plasmid

#196923

Purpose

The coding sequence of Triose phosphate isomerase gene was fused downstream Renilla in the ORF

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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IRE-nonOpt-TREAT

Plasmid

#196922

Purpose

The codon optimized Renilla coding sequence in the IRE-TREAT reporter was replaced by non-optimal Renilla containing 93 synonymous codon substitutions.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

non-optimal Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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Let7R-IRE-TREAT

Plasmid

#196934

Purpose

Reverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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