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pMST1212_BB2_E_BC_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90286

Purpose

BB2 with split pyrG marker for homologous integration with linker BC (for 1 expression cassette)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST1213_BB2_E_CD_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90287

Purpose

BB2 with split pyrG marker for homologous integration with linker CD (for 2 expression cassettes)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST1214_BB2_E_DE_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90288

Purpose

BB2 with split pyrG marker for homologous integration with linker DE (for 3 expression cassettes)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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Ppms028(JBEI-11601)

Plasmid

#87958

Purpose

jStack yeast assembly into plant vectors, Level 1 Intermediary Vector, p15A origin

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pms5102(JBEI-10536)

Plasmid

#87969

Purpose

jStack yeast assembly into plant vectors

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Insert

{L_AtHsp18.2}

Use

Synthetic Biology

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pms5055(JBEI-10543)

Plasmid

#87962

Purpose

jStack yeast assembly into plant vectors

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Insert

{L_tOcs}

Use

Synthetic Biology

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pms5092(JBEI-14430)

Plasmid

#87968

Purpose

jStack yeast assembly into plant vectors

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Insert

{T_AtHsp18.2}

Use

Synthetic Biology

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pms5056(JBEI-10534)

Plasmid

#87963

Purpose

jStack yeast assembly into plant vectors

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Insert

{T_tNos}

Use

Synthetic Biology

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pAcUW21-CqJHE

Plasmid

#84213

Purpose

baculovirus transfer vector containing cqjhe coding sequence from Culex quinquefasiatus

Depositor

Bruce Hammock

Article

Kamita et al PLoS One. 2011;6(12):e28392. doi: 10.137

Insert

JHE

Expression

Insect

Promoter

p10

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
ARK2a

Plasmid

#81017

Purpose

E.coli BW25113 + JBEI-9310 (pA5c-MevT(O)-T21-MKco, JBx_039538) + JBEI-9314(pTrc99a-PMDsc, JBx_039366)

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Inserts

MevTo-BBa1002-pTrc-Mkco
PMD

Promoter

LacUV5 and Trc promoter

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pJK040 (pET-PurQ-A128T)

Plasmid

#73840

Purpose

Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)

Depositor

T. J. Kappock, JoAnne Stubbe

Article

Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

Insert

formylglycinamidine ribonucleotide synthetase, glutaminase subunit

Expression

Bacterial

Mutation

A128T mutant

Promoter

T7

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pJK044 (pET-PurQ-wt)

Plasmid

#73839

Purpose

Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)

Depositor

T. J. Kappock, JoAnne Stubbe

Article

Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

Insert

formylglycinamidine ribonucleotide synthetase, glutaminase subunit

Expression

Bacterial

Promoter

T7

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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POT5-TDH3p-ycrtYB-ADH1t

Plasmid

#65358

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TDH3p-ycrtYB-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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POT2-TDH3p-ycrtE-ADH1t

Plasmid

#65352

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TDH3p-ycrtE-ADH1t

Use

Unspecified

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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MXS_PA-GFP

Plasmid

#62412

Purpose

MXS_chaining vector with PA-GFP

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

PA-GFP

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_loxP

Plasmid

#62430

Purpose

MXS_chaining vector with loxP

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

loxP site

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_linker

Plasmid

#62431

Purpose

MXS_chaining vector with linker

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Linker 12AA Gly-/Ser-rich

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_MCS4

Plasmid

#62398

Purpose

MXS_chaining vector with MCS4

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

MluI-XhoI-PvuII-BglII-SalI MCS

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pERB170

Plasmid

#58288

Purpose

const. Mis12-FKBP3; ind. FRB-mCH-Mad1^485-584

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-FKBPx3
inducible FRB-mCh-Mad1^485-584
FKBP-targeted miRNA

Tags

mCherry

Expression

Mammalian

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCVD403

Plasmid

#63740

Purpose

Contains the LT gene probe (HincII fragment) cloned on BamHI linkers into pACYC184

Depositor

James Kaper

Article

Gicquelais et al J Clin Microbiol. 1990 Nov;28(11):2485-9

Insert

LT gene probe

Expression

Bacterial

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only
SNAP-EF(N)-20nmSAH-GFP(1-10)

Plasmid

#61661

Purpose

20nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-20nmSAH-GFP(1-10)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pGLx2-set1-H1017K

Plasmid

#53258

Purpose

GAL driven LexA-tagged SET1-catalytically dead (H1017K)

Depositor

Jean Cook

Article

Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

Insert

SET1

Tags

LexA

Expression

Yeast

Mutation

H1017K mutation - catalytically dead

Promoter

GAL1/10

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pBbA5k-EPL54

Plasmid

#45417

Depositor

Aindrila Mukhopadhyay

Article

Dunlop et al Mol Syst Biol. 2011 May 10;7:487. doi: 1

Insert

Rmet_1702

Use

Synthetic Biology

Tags

His

Promoter

LacUV5

Available Since

Jan. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pBbA5k-EPL52

Plasmid

#45415

Depositor

Aindrila Mukhopadhyay

Article

Dunlop et al Mol Syst Biol. 2011 May 10;7:487. doi: 1

Insert

Rmet_4866

Use

Synthetic Biology

Tags

His

Promoter

LacUV5

Available Since

Jan. 24, 2014

Availability

Academic Institutions and Nonprofits only

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psiCheck2 6xmut UTR

Plasmid

#40764

Purpose

Renilla luciferase reporter containing siRNA target sites

Depositor

Phillip Zamore

Article

Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

Insert

6 copies (3 site expanded) of the CXCR4 small RNA target site

Use

Luciferase

Tags

luciferase

Promoter

TK/SV40

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
TAL3487

Plasmid

#47206

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-matrixmetalloproteinase14a(membrane-inserted)-right

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
TAL3486

Plasmid

#47205

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-matrixmetalloproteinase14a(membrane-inserted)-left

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
TAL3485

Plasmid

#47204

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-matrixmetallopeptidase15(membrane-inserted)-right

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
TAL3484

Plasmid

#47203

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-matrixmetallopeptidase15(membrane-inserted)-left

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
pBbA5k-EPL70

Plasmid

#45429

Depositor

Aindrila Mukhopadhyay

Article

Dunlop et al Mol Syst Biol. 2011 May 10;7:487. doi: 1

Insert

Gmet_1652

Use

Synthetic Biology

Tags

His

Promoter

LacUV5

Available Since

June 27, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbA5k-EPL69

Plasmid

#45428

Depositor

Aindrila Mukhopadhyay

Article

Dunlop et al Mol Syst Biol. 2011 May 10;7:487. doi: 1

Insert

Gmet_2518

Use

Synthetic Biology

Tags

His

Promoter

LacUV5

Available Since

June 27, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbA5k-EPL68

Plasmid

#45427

Depositor

Aindrila Mukhopadhyay

Article

Dunlop et al Mol Syst Biol. 2011 May 10;7:487. doi: 1

Insert

Gmet_1664

Use

Synthetic Biology

Tags

His

Promoter

LacUV5

Available Since

June 27, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
KHBD00420

Plasmid

#34527

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11301

Use

Gateway donor vector

Available Since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
pCGN R-Ras 38V, CVLS

Plasmid

#14735

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

Insert

R-ras (RRAS Human)

Tags

HA

Expression

Mammalian

Mutation

38V, CVLS

Available Since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only
pCGN R-Ras 38V, SVLL

Plasmid

#14736

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

Insert

R-ras (RRAS Human)

Tags

HA

Expression

Mammalian

Mutation

38V, SVLL

Available Since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only
pCGN R-Ras 38V, SPCVLL

Plasmid

#14737

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

Insert

R-ras (RRAS Human)

Tags

HA

Expression

Mammalian

Mutation

38V, SPCVLL

Available Since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only
pRSET FLII282Pglu-4m

Plasmid

#13564

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A T282S-ECFP-N283R

Tags

Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pBbE8k-fcs

Plasmid

#83068

Purpose

Expresses Fcs under pBAD regulation

Depositor

Matthew Chang

Article

Lo et al Cell Syst. 2016 Aug;3(2):133-43. doi: 10

Insert

fcs

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_RO1_8

Plasmid

#158013

Purpose

Expression vector for the de novo designed protein Expression_vector_RO1_8

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO1_8

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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Expression_vector_RO1_9

Plasmid

#158014

Purpose

Expression vector for the de novo designed protein Expression_vector_RO1_9

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO1_9

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

Enlarge

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