We narrowed to 25,965 results for: Vit;

1. pX330A-nBRD4/PITCh

   Plasmid

   #91794

   Purpose

   PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

   Depositor

   Behnam Nabet, James Bradner

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken β-actin promoter

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hADM-RFP

   Plasmid

   #22922

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 . 3():19.

   Insert

   human adrenomedullin promoter driving DsRedExpress (ADM Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-RAM-d2TTA::TRE-FLEX-tdTomato-WPREpA

   Plasmid

   #84468

   Purpose

   pAAV-CRAM-tdTomato

   Depositor

   Yingxi Lin

   Article

   Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   T230I (please see depositor comments below)

   Promoter

   TRE

   Available since

   Dec. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG/ATP11C(E184Q)-HA

   Plasmid

   #209251

   Purpose

   Mammalian expression of ATP11C mutant E184Q

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

   Insert

   ATP11C (ATP11C Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E184Q

   Promoter

   Available since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG/ATP8A1(E191Q)-HA

   Plasmid

   #209240

   Purpose

   Mammalian expression of ATP8A1 mutant E191Q

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

   Insert

   ATP8A1 (ATP8A1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E191Q

   Promoter

   Available since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEM-Cas9HF1-recA56

   Plasmid

   #89962

   Purpose

   Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Inserts

   Cas9HF1
   recA56
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   pTet and proD

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLVX-CMV-Galpha-z ONE-GO

   Plasmid

   #189734

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Inserts

   Galpha-z ONE-GO
   GNAZ (Gnaz Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pB-TAG-NICD

   Plasmid

   #130934

   Purpose

   PiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cells

   Depositor

   James Thomson

   Article

   Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

   Insert

   NICD (NOTCH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pICE-HA-MRE11-H129N

   Plasmid

   #82034

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of H129N nuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Patrick Calsou, Sébastien Britton

   Article

   Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

   Insert

   MRE11 (MRE11 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Nuclease-dead mutant: H129N MRE11

   Promoter

   CMV-tet

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLVX-CMV-Galpha-oB ONE-GO

    Plasmid

    #189733

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-oB ONE-GO
    GNAO (Gnao1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFB1.HMBP.PrS.PHF19

    Plasmid

    #125168

    Purpose

    Expresses human PHF19 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    PHF19 (PHF19 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pICE-HA-MRE11-H63D

    Plasmid

    #82036

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of H63D exonuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    MRE11 (MRE11 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Exonuclease-dead mutant: H63D MRE11

    Promoter

    CMV-tet

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV4a-Flag-c-Myc

    Plasmid

    #102625

    Purpose

    Expresses c-Myc in mammalian cells

    Depositor

    Hening Lin

    Article

    Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEM-Cas9HF1-indRecA56

    Plasmid

    #102294

    Purpose

    Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Inserts

    Cas9HF1
    recA56
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    pTet

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLentiPGK Puro DEST ERKKTRClover

    Plasmid

    #90227

    Purpose

    Lentiviral vector to express ERK KTR mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    ERK Kinase Translocation Reporter
    

    Use

    Lentiviral

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SPLICS NU-MT P2A

    Plasmid

    #210129

    Purpose

    Detect the Nucleus-Mitochondria contact

    Depositor

    Tito Calì

    Article

    Wu et al EMBO J. 2022 Dec 15;41(24):e112006. doi:

    Insert

    SPLICS NU-MT P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-FLAG-MTOR-L1460P

    Plasmid

    #69006

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-L1460P (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Leucine 1460 to Proline

    Promoter

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP‐RNASEH1

    Plasmid

    #108699

    Purpose

    For expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cells

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH1 (nuclear isoform) (RNASEH1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPRKCZ-C1-EGFP (C-PRKCZ-C1)

    Plasmid

    #217759

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Protein kinase C zeta (PRKCZ Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C1 domain (aa 123-193)

    Promoter

    CMV

    Available since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pBbA5a-sufABCDSE

    Plasmid

    #195056

    Purpose

    Expresses SUF operon from E. coli from IPTG-inducible promoter

    Depositor

    Greg Bokinsky

    Article

    D'Angelo et al Elife. 2022 Mar 4;11:e70936. doi: 10.755

    Insert

    sufABCDSE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV.Syn.NES-jRCaMP1b.WPRE.SV40

    Plasmid

    #100851

    Purpose

    AAV expression of jRCaMP1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1 and AAV9

    Insert

    jRCaMP1b
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 416 pSG5L HA RB del22

    Plasmid

    #10721

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB del22 (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletion of exon 22 (aa738-775)

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

23. pDB1 (CK2alpha)

    Plasmid

    #27083

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2alpha (CSNK2A1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Cterminal HA WDR59 pRK5

    Plasmid

    #46328

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    WDR59 (WDR59 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LV-LIN28A

    Plasmid

    #117057

    Purpose

    Overexpression of mouse LIN28A

    Depositor

    Michele De Palma

    Article

    Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

    Insert

    LIN28A (Lin28a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hPGK promoter

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV CMV mCherry-DDX1-V5

    Plasmid

    #175162

    Purpose

    Lentiviral expression of human DDX1-V5

    Depositor

    Larry Gerace

    Article

    Hammond et al J Mol Biol. 2018 Feb 16;430(4):537-553.

    Insert

    DDX1 (DDX1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-FLAG-MTOR-C1483F

    Plasmid

    #69008

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-C1483F (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Cysteine 1483 to Phenylalanine

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

28. non-targeting control gRNA (BRDN0001149198)

    Plasmid

    #80248

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAB46 (CK2beta)

    Plasmid

    #27085

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2beta (CSNK2B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA

    Plasmid

    #84474

    Purpose

    pAAV-RAM-NLS-mKate2

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Insert

    mKate2
    

    Use

    AAV

    Tags

    Nuclear localization signal of SV40 large T antig…

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAJ075_hsCRBN

    Plasmid

    #124214

    Purpose

    Insect cell expression vector for His6 tagged hsCRBN

    Depositor

    Eric Fischer

    Article

    Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

    Insert

    Cereblon (CRBN Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-pTH-iCre:EGFP-WPREpA

    Plasmid

    #213142

    Purpose

    Truncated rat TH promoter expressing iCre fused to EGFP

    Depositor

    Ulrik Gether

    Article

    Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

    Insert

    iCre
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated TH promoter from rat (Rattus norvegicus…

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBABE-HA-GOLPH3

    Plasmid

    #21687

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Golgi Phosphoprotein 3 (GOLPH3 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-Ef1a-fDIO-tdTomato

    Plasmid

    #128434

    Purpose

    Expresses tdTomato under control of eukaryotic Ef1a promoter after Flp-dependent recombination

    Depositor

    Patricia Jensen

    Article

    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

    Has Service

    AAV1

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pC1_oROS-HT

    Plasmid

    #216413

    Purpose

    Expresses the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLV-SspB-HaloTag-TIAM1-DHPH

    Plasmid

    #176127

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHAGE-FLAG-APEX2-LGALS3

    Plasmid

    #175756

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS3

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-Ef1a-fDIO-ChrimsonR-tdTomato

    Plasmid

    #171027

    Purpose

    Expresses ChrimsonR-tdTomato fusion after Flp-dependent recombination

    Depositor

    Patricia Jensen

    Article

    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

    Has Service

    AAV1 and AAV9

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    ChrimsonR is fused to tdTomato

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGEX/PD-L1 WT

    Plasmid

    #121466

    Purpose

    Expression of GST-human PD-L1 in E. Coli

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. STARR-seq luciferase validation vector_ORI_neg.cont

    Plasmid

    #99315

    Purpose

    Negative control for luciferase validation vectors

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    neutral region; chr3: 55198740-55200041
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge