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We narrowed to 24,286 results for: Sis

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Showing: 4141 - 4160 of 24286 results

  1. pRL_GI_PCB28

    Plasmid
    #98745
    Purpose
    For phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919
    Inserts
    heme oxygenase 1 (TED4 Mustard Weed)
    Phycocyanobilin:ferredoxin oxidoreductase
    Use
    Synthetic Biology
    Expression
    Yeast
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    ADH1m and PGK1
    Available Since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98745-plasmid-map-sequence-id-189854

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  10. pCAGGS-PHFF

    Plasmid
    #100280
    Purpose
    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Insert
    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    Tags
    FLAG, T7 and Mitochondria-targeting sequence (MTS…
    Expression
    Mammalian
    Mutation
    All genes are codon-optimized for expression in h…
    Promoter
    CAG promoter
    Available Since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    100280-plasmid-map-sequence-id-194335

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  14. pNOC_dCas9_sgRNA NC

    Plasmid
    #176259
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176259-plasmid-map-sequence-id-346581

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  16. pICE-HA-CtIP-siR-N289A-H290A

    Plasmid
    #82032
    Purpose
    Plasmid for constitutive or doxy-inducible expression of N289A+H290A mutant of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Insert
    CtIP (RBBP8 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Endonuclease-dead mutant: N289A+H290A CtIP; Mutat…
    Promoter
    CMV-tet
    Available Since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82032-plasmid-map-sequence-id-155382

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  18. pICE-HA-CtIP-siR-S664A-S679A-S745A

    Plasmid
    #82031
    Purpose
    Plasmid for constitutive or doxy-inducible expression of S664A+S679A+S745A human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Insert
    CtIP (RBBP8 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Non phosphorylatable mutations on 3 of the main A…
    Promoter
    CMV-tet
    Available Since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82031-plasmid-map-sequence-id-155380

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  19. sgSf3b1(T1)

    Plasmid
    #90424
    Purpose
    induces dsDNA break within mouse Sf3b1 gene for subsequent homology-directed recombination and coding sequence mutagenesis at codon 700
    Depositor
    Alex Minella
    Article
    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519
    Insert
    mus Sf3b1 gRNA (Sf3b1 Mouse)
    Use
    CRISPR; Guiderna encoding for gene editing
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    90424-plasmid-map-sequence-id-190772

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  20. pNOC_dfnCas12a_crRNA NC

    Plasmid
    #176256
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and spacer sequence is replaced by the type IIS restriction site for endonuclease BaeI that can be
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176256-plasmid-map-sequence-id-346577

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Showing: 4141 - 4160 of 24286 results