We narrowed to 11,655 results for: pcas

1. pCAGGS-3xFLAG-bIV spectrin (repeats 13-15)

   Plasmid

   #213728

   Purpose

   Mammalian cell expression vector for overexpression of bIV spectrin (repeats 13-15) in NIH/3T3 cells.

   Depositor

   Chaolin Zhang

   Article

   Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

   Insert

   bIV spectrin (repeats 13-15) (Sptbn4 Mouse)
   

   Expression

   Mammalian

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

   Plasmid

   #103866

   Purpose

   dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Inserts

   PspCas13b
   huADAR2DD (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   GSGGGGS and HIV NES

   Expression

   Mammalian

   Mutation

   Changed histidne 133 to alanine and histidine 105…

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

   Plasmid

   #126706

   Purpose

   to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

   Insert

   ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
   

   Use

   CRISPR

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-pCALM1-EYFP-WPRE-bGHpA

   Plasmid

   #213971

   Purpose

   AAV expression of EYFP from pCALM1 promoter

   Depositor

   Zhonghua Lu

   Article

   Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

   Insert

   pCALM1
   

   Use

   AAV

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-Akt-FoxO3a-KTR-EGFP

   Plasmid

   #125131

   Purpose

   Fluorescent reporter for Akt activity

   Depositor

   Kazuhiro Aoki

   Article

   Maryu et al Cell Struct Funct. 2016 Jul 22;41(2):81-

   Insert

   FOXO2 (FOXO3 Human)
   

   Tags

   Enhanced green fluorescent protein (EGFP)

   Expression

   Mammalian

   Mutation

   FoxO3A 1-402 amino acid

   Promoter

   CAG promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV pCAG-FLEX-tdTomato-WPRE

   Plasmid

   #51503

   Purpose

   Can be used to generate AAV virus that will express tdTomato in the presence of Cre

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Insert

   tdTomato
   

   Use

   AAV

   Promoter

   pCAG

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-SpCas9(D10A)-T2A-EGFP

   Plasmid

   #221230

   Purpose

   Express nCas9 nickase (D10) with GFP reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   SpCas9(D10A)-T2A-EGFP
   

   Expression

   Mammalian

   Mutation

   D10A

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGEN-Dre-nls (JDW 1131)

   Plasmid

   #229809

   Purpose

   A CAGGS-driven dre recombinase followed by a c-terminal 1x nls sequence

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   DRE recombinase-nls
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT3TS-spCas9-ABE-Umax-rest1

   Plasmid

   #222144

   Purpose

   Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Bipartite NLS

   Promoter

   T3

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid

    #106477

    Purpose

    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT3TS-spCas9 ABE-Umax-ex1

    Plasmid

    #222141

    Purpose

    Adenine base editor with modified TadA variant for extended window for A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Promoter

    T3

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV_hUbC-dSpCas9-2xVP64-T2A-BSD

    Plasmid

    #162333

    Purpose

    Lentiviral expression of dSpCas9-2xVP64 with blasticidin resistance

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    dSpCas9-2xVP64
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV pCAG-FLEX-mScarlet-WPRE

    Plasmid

    #99280

    Purpose

    Can be used to generate AAV virus that will express mScarlet in the presence of Cre

    Depositor

    Rylan Larsen

    Insert

    mScarlet
    

    Use

    AAV and Cre/Lox

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. CROPseq-EFS-HypaSpCas9-P2A-puro

    Plasmid

    #106965

    Purpose

    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

    Depositor

    Alex Hewitt

    Insert

    HypaSpCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG PSAM4 5HT3 LC IRES GFP

    Plasmid

    #123066

    Purpose

    Chemogenetic activator expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Insert

    PSAM4 5HT3 LC IRES GFP (Htr3a Mouse)
    

    Expression

    Mammalian

    Mutation

    L131G Q139L Y217F

    Promoter

    CAG

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-SpCas9(H840A)-T2A-EGFP

    Plasmid

    #221231

    Purpose

    Express nCas9 nickase (H840A) with GFP reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(H840A)-T2A-EGFP
    

    Expression

    Mammalian

    Mutation

    H840A

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2-3xFLAG-NLS-SpCas9-NLS

    Plasmid

    #51307

    Purpose

    Cas9 expression plasmid

    Depositor

    Yonglong Chen

    Article

    Guo et al Development. 2014 Jan 8.

    Insert

    3xFLAG-NLS-SpCas9-NLS
    

    Use

    CRISPR; Xenopus tropicalis expression

    Tags

    3x Flag and NLS

    Promoter

    CMV

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-GPHN-FingR-mGold2s-CCR5TC

    Plasmid

    #231785

    Purpose

    Visualization of inhibitory post-synapse using GPHN-FingR-mGold2s

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    GPHN-FingR-mGold2s-CCR5TC
    

    Expression

    Mammalian

    Promoter

    Actin promoter with CMV enhancer

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. phU1-PspCas13b-non-targeting (pEZ36)

    Plasmid

    #229053

    Purpose

    Cloning backbone for PspCas13b crRNA (Human U1 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-CBE4max-SpRYc-P2A-EGFP

    Plasmid

    #208336

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc

    Depositor

    Pranam Chatterjee

    Article

    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

    Insert

    human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    Sc++ (1-1119), SpRY (1111-1368), D10A

    Promoter

    CAG

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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