We narrowed to 5,945 results for: pes;

1. pSLQ1371-sgITGB4-1

   Plasmid

   #121535

   Purpose

   sgITGB4-1 sequence: GAGGCGCAGTCCTTATCCACA. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

   Depositor

   Ovijit Chaudhuri, Stanley Qi

   Article

   Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

   Insert

   sgITGB4-1
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB3053(gRNA X-2, XI-5, XII-4)

   Plasmid

   #73295

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-2, XI-5, and XII-4

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ1371-sgITGB1-3

   Plasmid

   #121534

   Purpose

   sgITGB1-3 sequence: GTTCAGTGAATGGGAACAACG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

   Depositor

   Ovijit Chaudhuri, Stanley Qi

   Article

   Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

   Insert

   sgITGB1-3
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-H031-QES.i07.c09

   Plasmid

   #123271

   Purpose

   Mammalian expression plasmid for Env from the H031 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopes

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (H031) Env
   

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; Mutations S49D, F…

   Promoter

   CMV

   Available Since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP4-CH111-QES.c13.L544Y

   Plasmid

   #123267

   Purpose

   Mammalian expression plasmid for Env from the CH111 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopes

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (AD8) Env
   

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; Mutations V208M, …

   Promoter

   CMV

   Available Since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP4-CH115gp160-QES.c14

   Plasmid

   #123279

   Purpose

   Mammalian expression plasmid for Env from the CH115 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopes

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (CH115) Env
   

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; Mutations V208M, …

   Promoter

   CMV

   Available Since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-HRE-dUnaG

   Plasmid

   #124372

   Purpose

   UnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signaling

   Depositor

   Roland Friedel

   Article

   Sattiraju et al Immunity. 2023 Jul 7:S1074-7613(23)00274

   Insert

   HRE-dUnaG
   

   Use

   Lentiviral

   Tags

   PEST-degron and Myc tag

   Promoter

   HRE (HIF responsive element 5x + CMV minimal prom…

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-NLS-CBGreen-FIRE-puro

   Plasmid

   #139195

   Purpose

   Luminescent reporter of ERK activity

   Depositor

   Matthew Lazzara

   Article

   Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

   Insert

   CBG99
   

   Use

   Luciferase and Retroviral

   Tags

   Fra1 PEST domain fragment (FIRE) and nuclear loca…

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCVL Traffic Light Reporter 2.1 (VF2468 ZFN target) Ef1a

   Plasmid

   #31483

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Traffic Light Reporter 2.1 (V2468 zfn target) EF Puro
   

   Use

   Lentiviral

   Mutation

   Traffic Light 2.0 series have a +3 PEST sequence …

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. Fireworks RFP[PTC+] (AVA2626)

    Plasmid

    #85444

    Purpose

    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
    

    Use

    Minimal backbone fragment for low-copy bacterial …

    Expression

    Mammalian

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUAST-attB-3xHA-V5-RpL10Ab

    Plasmid

    #125223

    Purpose

    3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression

    Depositor

    Herman Dierick

    Article

    Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

    Insert

    RpL10Ab (RpL10Ab Fly)
    

    Tags

    3x HA, V5

    Expression

    Insect

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-CH115gp160-QES.c14-754*

    Plasmid

    #123282

    Purpose

    Mammalian expression plasmid for Env from the CH115 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (CH115) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Premature stop co…

    Promoter

    CMV

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT2-7xTcf-NLS-ONL-CP

    Plasmid

    #65716

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, ONL, hCL1-PEST
    

    Use

    Luciferase

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT2-7xTcf-NLS-YNL-CP

    Plasmid

    #65714

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, YNL, hCL1-PEST
    

    Use

    Luciferase

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2-7xTcf-NLS-CNL-CP

    Plasmid

    #65715

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, CNL, hCL1-PEST
    

    Use

    Luciferase

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. sgFFL_3x_siRNA

    Plasmid

    #59893

    Purpose

    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with three siRNA-like sites

    Depositor

    Georg Seelig

    Article

    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.

    Insert

    mCherry with intron containing the mouse mir-124–3
    

    Tags

    PEST and VAMP 3 UTR

    Expression

    Mammalian

    Mutation

    VAMP 3 UTR--has three siRNA-like targets (fully c…

    Promoter

    doxycycline (Dox)-inducible promoter (CMV/TO)

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEP4-CH111-QES.c13.L544Y-754*

    Plasmid

    #123264

    Purpose

    Mammalian expression plasmid for Env from the CH111 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (AD8) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Premature stop co…

    Promoter

    CMV

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCEP4-AD8-QES.i08.c04-754*

    Plasmid

    #123258

    Purpose

    Mammalian expression plasmid for Env from the AD8 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (AD8) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Premature stop co…

    Promoter

    CMV

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCEP4-SHIV327C-HuA10-QES.i09.c07

    Plasmid

    #123269

    Purpose

    Mammalian expression plasmid for Env from the SHIV327C-Hu A10 strain; QES mutant for enhanced presentation of quaternary epitopes

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    SHIV327C-Hu A10 Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Mutations V208M, …

    Promoter

    CMV

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a_6HT-TBP6.7(R52A)

    Plasmid

    #112729

    Purpose

    52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R52A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R52A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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