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pL1P5 ZmUbiP:GRF-GIF:NosT

Plasmid

#198046

Purpose

MoClo Golden Gate Level 1 Position 5. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

Depositor

Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

Insert

ZmUbiP::TaGRF4-GIF1::NosT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Zea mays (maize) ubiquitin promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLC-ZsGreen-P2A-Blast

Plasmid

#123322

Purpose

Lentiviral vector for expression of ZsGreen-P2A-Blast cassette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

ZsGreen

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCB301TOR-CRISPR

Plasmid

#164797

Purpose

For gene editing in oomycetes

Depositor

Miaoying Tian

Article

Gumtow et al Mol Plant Microbe Interact. 2018 Mar;31(

Inserts

PsNLS-hSpCas9
sgRNA expression cassette

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

HAM34 and RPL41

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCBC-MT3T4

Plasmid

#50595

Purpose

CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Insert

T3, gRNA scaffold, U6-26t plus, OsU3p, T4

Use

CRISPR; Pcr template

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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B52_puro_empty_gRNA

Plasmid

#197557

Purpose

sites for cloning two gRNA with puromycin cassette

Depositor

Darius Bägli

Type

Empty backbone

Use

Empty grna backbone with puromycin resistance gene

Tags

Expression

Mammalian

Mutation

Promoter

U6 for gRNA, CMV for puromycin resistance.

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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c3GIC9

Plasmid

#62191

Purpose

col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes

Depositor

Lukas Dow

Article

Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

Insert

humanized S. Pyogenes Cas9

Use

CRISPR

Tags

3x FLAG

Expression

Mutation

Promoter

TRE3G

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWR

Plasmid

#112900

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of tdTomato.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35S

Plasmid

#211839

Purpose

Plant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

HR1b

Use

Synthetic Biology

Tags

GFP

Expression

Plant

Mutation

R181A R185A

Promoter

UBIQUITIN10

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos

Plasmid

#211841

Purpose

Plant transient expression cassette for the E3-DART ligase containing a nuclear localization signal.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

3xNLS-HA and mCherry

Expression

Plant

Mutation

Promoter

UBIQUITIN10

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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His6-PRC1

Plasmid

#69111

Purpose

For bacterial expression of recombinant human PRC1 tagged with His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Protein Regulator of Cytokinesis 1 (PRC1 Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pMGS58

Plasmid

#135311

Purpose

A HygR-P2A-AID cassette to tag the protein of interest with auxin-inducible degron at the n-terminal

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

HygR-P2A-AID

Use

N terminal tagging plasmid

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::PuroR-bGHpA

Plasmid

#62439

Purpose

MXS_chaining vector with CMV::PuroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Puromycin with CMV Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMC_mClover3_BSDminus_F0

Plasmid

#184165

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mClover3
bsd

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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psiTE(2)-Gal4

Plasmid

#220451

Purpose

Split intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(0)-Gal4DBD

Plasmid

#220452

Purpose

Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4DBD-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(2)-Gal4DBD

Plasmid

#220454

Purpose

Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4DBD-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(1)-p65AD

Plasmid

#220456

Purpose

Split intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-p65AD-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(0)-Gal4

Plasmid

#220449

Purpose

Split intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(2)-LexAGAD

Plasmid

#220460

Purpose

Split intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-LexAGAD-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(1)-Gal4DBD

Plasmid

#220453

Purpose

Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4DBD-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(1)-LexAGAD

Plasmid

#220459

Purpose

Split intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-LexAGAD-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(0)-p65AD

Plasmid

#220455

Purpose

Split intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-p65AD-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(1)-Gal4

Plasmid

#220450

Purpose

Split intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(0)-LexAGAD

Plasmid

#220458

Purpose

Split intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-LexAGAD-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(2)-p65AD

Plasmid

#220457

Purpose

Split intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-p65AD-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pSGKP_AcsgRNA

Plasmid

#203807

Purpose

pSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

J23119

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLC-ZsGreen-P2A-Hygro

Plasmid

#124301

Purpose

Lentiviral vector for expression of ZsGreen-P2A-Hygro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

ZsGreen

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLD-puro-CcVA

Plasmid

#24588

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

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IZ501: pMVP/Lenti-DEST

Plasmid

#121848

Purpose

pMVP destination vector, empty Lentivirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Lentiviral; Pmvp gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pXZX501

Plasmid

#169455

Purpose

pRS426-SNR52p-gRNA.MATa_option3-SUP4t; 20-bp gRNA sequence targeting MATa cassette

Depositor

Jef Boeke

Article

Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

Insert

gRNA for MATa

Use

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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