We narrowed to 4,564 results for: ARA-2

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pScalps_Puro_mTet2 catalytic domain HxD

Plasmid

#79611

Purpose

Expression of catalytically inactive mouse Tet2

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet2 (Tet2 Mouse)

Use

Lentiviral

Tags

Myc

Mutation

Mutant Tet2 with H1302Y, D1304A substitutions in …

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
AICSDP-1:PXN-EGFP

Plasmid

#87420

Purpose

Homology arms and linker-EGFP sequence for C-terminus tagging of human PXN

Depositor

Allen Institute for Cell Science

Article

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

PXN Homology Arms with linker-EGFP (PXN Human)

Use

CRISPR; Donor template

Tags

linker-EGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

March 15, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pVD1 tRNA-gRNA position E2 (GB2239)

Plasmid

#160561

Purpose

tRNA and scaffold for the assembly of GBoligomers for position [2-3] of a polycistronic tRNA-gRNA

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

tRNA-gRNA position E2 (Multiplexing Edit)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD S1859A

Plasmid

#169821

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

S1859A; TCCC -> AGTC silent mutations at nt527…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-OptoFGFR1-D387A

Plasmid

#59778

Purpose

Expresses optoFGFR1 with mutation in PHR to suppress homointeraction, thus cannot be activated by light

Depositor

Won Do Heo

Article

Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

Insert

OptoFGFR1-D387A (FGFR1 Mustard Weed, Human)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4 h-Stx3 resistant to shRNA #304-2xMyc/His

Plasmid

#99746

Purpose

Expresses human Stx3 with a C-term myc-myc-his that is resistant to shRNA #304 from Sigma Aldrich.

Depositor

Thomas Weimbs

Article

Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

Insert

Stx3 (STX3 Human)

Tags

myc-myc-his

Expression

Mammalian

Mutation

Made six (6) silent mutations to confer resistanc…

Promoter

CMV/TO

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR

Plasmid

#170808

Purpose

PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Inserts

GID1-VPR-IRES-HygR
GAI-tagBFP-Sa_dCas9 (GAI S. aureus, Mustard Weed, Synthetic)

Expression

Mammalian

Mutation

Synonymous mutation in PYL1 to remove XhoI site (…

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-ZNF207_∆RNA1-2_-3xFLAG

Plasmid

#231933

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆RNA1-2_-3xFLAG

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al bioRxiv [Preprint]. 2025 Apr 26:2025.04.

Insert

ZNF207 (ZNF207 Human)

Tags

FLAG

Expression

Mammalian

Mutation

deletion of RNA1-2

Promoter

CMV/TO inducible promoter

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pTW368z

Plasmid

#246581

Purpose

Golden gate acceptor for AtRaf1 library cloning

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

sfGFP, AtRaf2, AtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (RbcX2, CPN60B, CPN20, CPN60A Mustard Weed)

Expression

Bacterial

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_hnRNPA1_allW

Plasmid

#224252

Purpose

Bacterial expression of N-terminally 6His-tagged hnRNPA1_allW

Depositor

Tanja Mittag

Article

Alshareedah et al Nat Phys. 2024 Sep;20(9):1482-1491. doi:

Insert

hnRNPA1_allW (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

F17W,F25W,F31W,F37W,F43W,Y52W, Y59W, F62W, F69W, …

Promoter

T7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD M1601E

Plasmid

#169828

Purpose

Expresses C-terminal flag-tagged CAD with mutation at reported dimerization interface of the DHOase domain

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

M1601E; TCCC -> AGTC silent mutations at nt527…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD S1859E

Plasmid

#169822

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

S1859E; TCCC -> AGTC silent mutations at nt527…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7_noCoreStab

Plasmid

#248014

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&PROSS&ActiveSite stability)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_noCoreStab

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7_PROSS

Plasmid

#248012

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&PROSS)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_PROSS

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pWormgate2-myo-2p-mCherry-P2A-flag-UltraID

Plasmid

#227172

Purpose

For organ-specific expression of separated mCherry and UltraID in the pharyngeal muscle of C. elegans.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

mCherry::P2A::UltraID

Expression

Worm

Promoter

myo-2

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-KRf-TetR

Plasmid

#216269

Purpose

Construct 2: Lipid binding domain KRφ - TetR

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

Lipid binding motif (KRf) - TetR

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST EGFP-flag-CAD

Plasmid

#188118

Purpose

Expresses N-terminal EGFP-tagged/C-terminal flag-tagged human CAD in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

EGFP tag and Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pPtGE35

Plasmid

#107999

Purpose

Expresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

Depositor

David Edgell

Article

Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

Inserts

OriT
40SRPS8 Promoter
ShBle
40SRPS8 Terminator
Cen6-ArsH4-His3
I-TevI nuclease and partial linker domain

Use

CRISPR and Synthetic Biology; Episomal vector for…

Tags

Cas9

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TRC.mKO2_shB3GNT5.1

Plasmid

#110325

Purpose

TRCN0000034809 (Target GCCTATGTAATCTCCGGTGAT), silence human B3GNT5 gene and express monomeric Kusabira-Orange2

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

B3GNT5 (B3GNT5 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST Halo-flag-CAD

Plasmid

#188117

Purpose

Expresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag and Halo tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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