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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,282 results for: LEA

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Showing: 4161 - 4180 of 12282 results

  5. TVBB C-term-mNeongreen-Blast

    Plasmid
    #169229
    Purpose
    Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Blast at the C-terminus of a target locus
    Depositor
    Hugo Snippert
    Publication
    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Double SapI flanked mNeongreen-P2A-Blast
    Use
    Targeting vector backbone
    Available since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    169229-plasmid-map-sequence-id-388042

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  7. pαH-S-HRV3C

    Plasmid
    #164567
    Purpose
    SARS-CoV-2 Spike protein with furin site mutated to HRV3C site (S-HRV3C variant)
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:
    Insert
    Spike - Furin site changed for HRV3C site
    Tags
    2X Strep-Tag II and 8X His tag
    Expression
    Mammalian
    Mutation
    682-685 (furin site) replaced with HRV3C site (LE…
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    164567-plasmid-map-sequence-id-324831

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  9. pGBW-m4133197

    Plasmid
    #152383
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 helicase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152383-plasmid-map-sequence-id-314728

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  10. pGBW-m4134251

    Plasmid
    #152087
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp7
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152087-plasmid-map-sequence-id-297920

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  11. pGBW-m4134072

    Plasmid
    #152082
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp4
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152082-plasmid-map-sequence-id-297918

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  12. pcDNA3.1(+)-sapphireAKAR(T/A)

    Plasmid
    #118465
    Purpose
    Negative-control mutant for sapphireAKAR biosensor.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sapphireAKAR(T/A)
    Tags
    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Mutation
    Contains Thr-to-Ala mutation in substrate sequenc…
    Promoter
    CMV
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    118465-plasmid-map-sequence-id-230227

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  14. pCMV-hAR1-660

    Plasmid
    #89079
    Purpose
    mammalian expression of human androgen receptor N-terminal fragment 1-660 amino acids
    Depositor
    Elizabeth Wilson
    Publication
    Simental et al J Biol Chem. 1991 Jan 5;266(1):510-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hAR-1-660 (AR Human)
    Expression
    Mammalian
    Mutation
    aa 1-660 (contains exons A,B,C and part of D)
    Promoter
    CMV
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    89079-plasmid-map-sequence-id-175227

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  18. pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)

    Plasmid
    #220611
    Purpose
    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.
    Depositor
    Sam Golden
    Publication
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-KORD-P2A-3x HA-hM3D(Gq)
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Promoter
    hSyn
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220611-plasmid-map-sequence-id-441218

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  19. pECFP_C1_NSD2 FL

    Plasmid
    #221382
    Purpose
    Expresses NSD2 in mammalian cells
    Depositor
    Albert Jeltsch
    Publication
    Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Histone-lysine N-methyltransferase NSD2 (NSD2 Human)
    Expression
    Mammalian
    Mutation
    L237R mutation in ECFP (Please see depositor comm…
    Promoter
    CMV
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    221382-plasmid-map-sequence-id-436975

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Showing: 4161 - 4180 of 12282 results