We narrowed to 15,789 results for: LEA

1. pcDNA3.1-V5-hTEV

   Plasmid

   #65800

   Purpose

   expresses human codon-optimised TEV protease

   Depositor

   Douglas Green

   Article

   Oberst et al J Biol Chem. 2010 May 28;285(22):16632-4

   Insert

   V5-human TEV
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   codon-optimised to express in mammalian cells

   Promoter

   CMV

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pTORKm43GWF

   Plasmid

   #112899

   Purpose

   Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mWasabi.

   Depositor

   Sebastian Schornack

   Article

   Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

   Type

   Empty backbone

   Use

   Oomycete expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDSAR-Cre-F2A-I-SceI

   Plasmid

   #62302

   Purpose

   Anopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed marker

   Depositor

   Eric Marois

   Article

   Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

   Insert

   Cre recombinase, I-SceI nuclease
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   vasa

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1 shFEN

   Plasmid

   #17921

   Depositor

   Sheila Stewart

   Article

   Saharia et al Curr Biol. 2008 Apr 8. 18(7):496-500.

   Insert

   shFEN (FEN1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pUDP293

   Plasmid

   #204228

   Purpose

   Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA

   Depositor

   Jean-Marc Daran

   Article

   Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

   Inserts

   Eubacterium rectale cas12a (Ercas12a)
   BsaI_GFP drop-out_BsaI
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mutation

   codon optimized for S. cerevisiae

   Promoter

   Saccharomyces cerevisiae PGK1 and Saccharomyces c…

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a_Pro-IL-37

   Plasmid

   #214346

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL37 (IL37 Human)
   

   Use

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-FLAG-PGRMC2

   Plasmid

   #173468

   Purpose

   Human PGRMC2 containing an N-terminal FLAG tag

   Depositor

   Bill Tsai

   Article

   Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

   Insert

   PGRMC2 (PGRMC2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL3E-hIL-17prom(-1125)-Luc

   Plasmid

   #25627

   Depositor

   Sarah Gaffen

   Article

   Liu et al J Biol Chem. 2004 Dec 10. 279(50):52762-

   Insert

   human IL-17 promoter (IL17A Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-EGFP-ELYS-polyA

   Plasmid

   #59746

   Purpose

   Express EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   EGFP-ELYS
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-emerin-EGFP-polyA

    Plasmid

    #59747

    Purpose

    Express emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    emerin-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-LBR-EGFP-polyA

    Plasmid

    #59748

    Purpose

    Express LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    LBR-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HA.PKD.K/W

    Plasmid

    #10809

    Depositor

    Alex Toker

    Article

    Storz et al J Biol Chem. 2003 May 16. 278(20):17969-

    Insert

    PKD (PRKD1 Human)
    

    Use

    Tags

    HA and Myc (inserted between amino acids 33&3…

    Expression

    Mammalian

    Mutation

    Kinase dead mutant: K612W (Lys 612 to Trp) (bp183…

    Promoter

    CMV

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-hBcan

    Plasmid

    #18966

    Depositor

    Mariano Viapiano

    Article

    Hu et al J Biol Chem. 2008 Sep 5;283(36):24848-59

    Insert

    Brevican (BCAN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

14. B52_puro_empty_gRNA

    Plasmid

    #197557

    Purpose

    sites for cloning two gRNA with puromycin cassette

    Depositor

    Darius Bägli

    Type

    Empty backbone

    Use

    Empty grna backbone with puromycin resistance gene

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for gRNA, CMV for puromycin resistance.

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ210

    Plasmid

    #109329

    Purpose

    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selection

    Depositor

    Yiping Qi

    Article

    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

    Type

    Empty backbone

    Use

    Gateway compatible attr1-attr2 destination vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p35S-GW-3Avi

    Plasmid

    #127918

    Purpose

    Gateway destination vector for transcient expression of proteins with C-terminal 3_Avi in plant protoplast

    Depositor

    Silin Zhong

    Article

    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

    Type

    Empty backbone

    Use

    Tags

    3xAviTag

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRG659_lexO-FseI_LexA-TF_LEU2MX

    Plasmid

    #154816

    Purpose

    Inducible expression of FseI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    FseI endonuclease
    LexA-ER-B112
    

    Use

    Tags

    FLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRG646_lexO-HO_LexA-TF_LEU2MX

    Plasmid

    #154815

    Purpose

    Inducible expression of HO endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    HO endonuclease (HO Budding Yeast)
    LexA-ER-B112
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-p53 (NES-)

    Plasmid

    #12092

    Depositor

    Tyler Jacks

    Article

    Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

    Insert

    p53 (NES-) (TP53 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L348A L350A Localized to nucleus.

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMSCV PIG IMP-1-short

    Plasmid

    #21659

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    IGF-II mRNA-binding protein 1 (IGF2BP1 Human)
    

    Use

    Retroviral

    Tags

    GFP and IRES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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