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pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)
Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tag
Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag
Leader Of Cox8 Repeated (LOC8R) is a synthetic mitochondrial targeting sequence compatible with any N1 vector. Internal restriction sites enable modular subcloning of 1x, 2x, 3x or 4x presequences.