We narrowed to 1,257 results for: LIPI

1. pLCKO_AAVS1_sgRNA_4

   Plasmid

   #155087

   Purpose

   lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LCV2_AAVS1_sgRNA_4

   Plasmid

   #155090

   Purpose

   lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBOB-N-Flag-GK (281-473)

   Plasmid

   #134273

   Purpose

   Lentivector encoding Flag-tagged GK truncation (amino acids 281-473)

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk (Gk Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   mouse Gk amino acids 281-473

   Promoter

   CMV

   Available since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBOB-N-Flag-GK (12-272)

   Plasmid

   #134272

   Purpose

   Lentivector encoding Flag-tagged GK truncation (amino acids 12-272)

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk (Gk Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   mouse Gk amino acids 12-272

   Promoter

   CMV

   Available since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBOB-N-Flag-GK_v1

   Plasmid

   #134270

   Purpose

   Lentivector encoding Flag-tagged GK (isoform 1)

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk (Gk Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   mouse Gk isoform 1; 524 amino acids

   Promoter

   CMV

   Available since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMBP-parallel1-GK5_v2 (D443N)

   Plasmid

   #134293

   Purpose

   Expresses GK5 (isoform 2)-D443N mutant in bacteria

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk5 (Gk5 Mouse)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed Aspartic Acid 443 to Asparagine

   Promoter

   T7

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMBP-parallel1-GK5_v2 (D280N)

   Plasmid

   #134290

   Purpose

   Expresses GK5 (isoform 2)-D280N mutant in bacteria

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk5 (Gk5 Mouse)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed Aspartic Acid 280 to Asparagine

   Promoter

   T7

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMBP-parallel1-GK5_v2 (D30N)

   Plasmid

   #134289

   Purpose

   Expresses GK5 (isoform 2)-D30N mutant in bacteria

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk5 (Gk5 Mouse)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed Aspartic Acid 30 to Asparagine

   Promoter

   T7

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA6-C-Flag-GK5_v1

   Plasmid

   #134281

   Purpose

   Expresses Flag-tagged GK5 (isoform 1) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk5 (Gk5 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   mouse Gk5 isoform 1; 516 amino acids

   Promoter

   CMV

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA6-GK5_v2

    Plasmid

    #134280

    Purpose

    Expresses GK5 (isoform 2) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mouse Gk5 isoform 2; 534 amino acids

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA6-GK5_v1

    Plasmid

    #134279

    Purpose

    Expresses GK5 (isoform 1) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mouse Gk5 isoform 1; 516 amino acids

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBOB-N-Flag-GK_v2

    Plasmid

    #134271

    Purpose

    Lentivector encoding Flag-tagged GK (isoform 2)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk (Gk Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk isoform 2; 553 amino acids

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBOB-C-3HA-GK5_v2

    Plasmid

    #134269

    Purpose

    Lentivector encoding 3XHA-tagged GK5 (isoform 2)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Lentiviral

    Tags

    3X HA

    Expression

    Mammalian

    Mutation

    mouse Gk5 isoform 2; 534 amino acids

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBOB-C-3HA-GK5_v1

    Plasmid

    #134268

    Purpose

    Lentivector encoding 3XHA-tagged GK5 (isoform 1)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Lentiviral

    Tags

    3X HA

    Expression

    Mammalian

    Mutation

    mouse Gk5 isoform 1; 516 amino acids

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBOB-C-Flag-GK5_v2

    Plasmid

    #134260

    Purpose

    Lentivector encoding Flag-tagged GK5 (isoform 2)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk5 isoform 2; 534 amino acids

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBOB-C-Flag-GK5_v1

    Plasmid

    #134257

    Purpose

    Lentivector encoding Flag-tagged GK5 (isoform 1)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk5 isoform 1; 516 amino acids

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-2TKN-bp1

    Plasmid

    #133428

    Purpose

    Human BP1 fragment of SREBF1 in vector for bacterial expression of GST fusion protein.

    Depositor

    Gerhard Wagner

    Article

    Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

    Insert

    SREBF1 (SREBF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    SREBP1a amino acids 1-50 fused to GST

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMDC43-FIT2-FLL[157-159]AAA

    Plasmid

    #96994

    Purpose

    Express GFP-tagged mutated mouse FIT2 gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

    Insert

    MmFIT2 (Fitm2 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    Amino acid residues 157-159 were mutated (FLL[157…

    Promoter

    35S

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMDC32-FIT2-FLL[157-159]AAA

    Plasmid

    #96991

    Purpose

    Express mouse FIT2 gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

    Insert

    MmFIT2 (Fitm2 Mouse)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Amino acid residues 157-159 were mutated (FLL[157…

    Promoter

    35S

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pICE-EGFP-FLAG-PSMD2

    Plasmid

    #161917

    Purpose

    Expresses human PSMD2 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    26S proteasome non-ATPase regulatory subunit 2 (PSMD2 Human)
    

    Use

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #129418

    Purpose

    Encoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminus

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    SpCas9 and sgRAB18.N (RAB18 Human)
    

    Use

    CRISPR

    Tags

    T2A-mCherry-P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDNA5_miniTurbo-C12orf49

    Plasmid

    #155111

    Purpose

    transfection plasmid for the exogneous expression of N-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    C12orf49 (SPRING1 Human)
    

    Use

    Tags

    miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO inducible promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mRFP-FKBP12-5ptpase domain

    Plasmid

    #67516

    Purpose

    Recruitable human type IV 5-phosphatase domain (214-644)

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

    Insert

    FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)
    

    Use

    Tags

    mRFP

    Expression

    Mutation

    INPP5E has C641A to destroy the C-terminal CAAX d…

    Promoter

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pAD-CMV-Caveolin1-CMV-GFP

    Plasmid

    #83272

    Purpose

    Adenoviral expression of Caveolin1 with co-expression of GFP

    Depositor

    Andrew Brooks

    Article

    Fernandez-Rojo et al Cell Rep. 2013 Jul 25;4(2):238-47. doi:

    Inserts

    CAV1 (CAV1 Human)
    eGFP
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-Lyso-INPP4B-mCherry

    Plasmid

    #184055

    Purpose

    Lysosome targeted inositol polyphosphate-4-phosphatase type II B; tagged with mCherry.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    LGP120 Lyso-INPP4B-mCherry (LAMP1, INPP4B Human)
    

    Use

    Tags

    Lysosome-associated membrane protein 1 (LAMP1). a…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDNA5_C12orf49-miniTurbo

    Plasmid

    #155112

    Purpose

    transfection plasmid for the exogneous expression of C-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    C12orf49 (SPRING1 Human)
    

    Use

    Tags

    3x Flag and miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO inducible promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Flag_HsIRE1a_deltaP29_D408_K599A_pBabePuro

    Plasmid

    #58492

    Purpose

    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408 and bearing mutation K599A (kinase domain mutant)

    Depositor

    David Ron

    Article

    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

    Insert

    IRE1a (ERN1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and preprotrypsin signal sequence

    Expression

    Mammalian

    Mutation

    deleted amino acids P29 to D408 and bearing mutat…

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mRFP-FKBP12-5ptpase enzyme

    Plasmid

    #67515

    Purpose

    Recruitable human type IV 5-phosphatase enzyme

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

    Insert

    FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    INPP5E has C641A to destroy the C-terminal CAAX d…

    Promoter

    CMV

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pETM30-ARC105

    Plasmid

    #133426

    Purpose

    Human ARC105 coding sequence in vector for in vitro transcription and protein expression, with T7 promoter.

    Depositor

    Gerhard Wagner

    Article

    Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

    Insert

    PCQAP (MED15 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains amino acids 5-78 fused to C-terminal of …

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGL3-sgACSL3.C-Cas9-P2A-Puro

    Plasmid

    #129412

    Purpose

    Encoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminus

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    SpCas9 and sgACSL3.C (ACSL3 Human)
    

    Use

    CRISPR

    Tags

    P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGL3-Basic-ACSL3-sfGFP(C) HDR template

    Plasmid

    #129413

    Purpose

    HDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFP

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    ACSL3 HDR template (ACSL3 Human)
    

    Use

    CRISPR and TALEN; Endogenous tagging hdr template

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGEX-2TK-arc105

    Plasmid

    #133425

    Purpose

    Human ARC105 coding sequence in vector for bacterial expression of GST fusion protein.

    Depositor

    Gerhard Wagner

    Article

    Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

    Insert

    PCQAP (MED15 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains amino acids 1-95 or ARC105 fused to the …

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pICE-EGFP-FLAG-TK1

    Plasmid

    #161919

    Purpose

    Expresses human TK1 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    Thymidine kinase, cytosolic (TK1 Human)
    

    Use

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Flag_MmPERK_S503_N1114_pBabePuro

    Plasmid

    #58423

    Purpose

    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant mouse PERK deleted from amino acids M1 to Y502. The remaining amino acids are S503 to N1114.

    Depositor

    David Ron

    Article

    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

    Insert

    PERK (Eif2ak3 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG and preprotrypsin signal sequence

    Expression

    Mammalian

    Mutation

    Deleted amino acids M1 to Y502

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. LCV2_PSMB2_sgRNA_1

    Plasmid

    #155092

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting PSMB2 (core essential gene)

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    PSMB2_sgRNA_1 (PSMB2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. LCV2_PSMD1_sgRNA_1

    Plasmid

    #155091

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting PSMD1 (core essential gene)

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    PSMD1_sgRNA_1 (PSMD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. LCV2_mCherry_PSMD1_sgRNA_1

    Plasmid

    #155107

    Purpose

    lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting PSMD1 (core essential gene)

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    PSMD1_sgRNA_1 (PSMD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-Lyso-InPTapp

    Plasmid

    #236000

    Purpose

    Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4)P2. Targeted to lysosome surface.

    Depositor

    Jin Zhang

    Article

    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

    Insert

    Lyso-InPTapp (PLEKHA1, LAMP1 Synthetic, Human)
    

    Use

    Tags

    Citrine, Full-length LAMP1, and truncECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-Lyso-INPP5E

    Plasmid

    #236004

    Purpose

    Lysosome-targeted inositol polyphosphate 5 phosphatase (INPP5E); hydrolyzes 5-phosphate in PI(3,4,5)P3 and PI(4,5)P2.

    Depositor

    Jin Zhang

    Article

    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

    Insert

    Lyso-INPP5E (LAMP1, INPP5E Synthetic, Human)
    

    Use

    Tags

    Full-length LAMP1 and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pE-N1-RDH11

    Plasmid

    #161916

    Purpose

    Expresses untagged human RDH11. Confers G418 resistance.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    Retinol dehydrogenase 11 (RDH11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCAG-eSpCas9-2A-GFP-sgRNA-RDH11

    Plasmid

    #161924

    Purpose

    To generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    sgRNA targeting RDH11 exon 2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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42. pMXS-IRES-Blast HA-GPAT4-ΔNs5

    Plasmid

    #173169

    Purpose

    Retroviral vector to express sgRNA resistant HA tagged human GPAT4 with mutation in CHP1 binding site

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Glycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Synonymous mutations at sgRNA sites, amino acids …

    Promoter

    MMLV

    Available since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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43. pMXS-IRES-Blast 3XFLAG-CHP1-myr_mut

    Plasmid

    #173167

    Purpose

    Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1 with myristoylation site mutation

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Calcineurin Like EF-Hand Protein 1 (CHP1 Human)
    

    Use

    Retroviral

    Tags

    3XFLAG

    Expression

    Mutation

    Synonymous mutations at sgRNA sites, G2A, S6A

    Promoter

    MMLV

    Available since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pMBP-parallel1-GK5_v2 (D30N+D280N+D443N)

    Plasmid

    #134291

    Purpose

    Expresses GK5 (isoform 2)-D30N/D280N/D443N mutant in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed Aspartic Acids 30, 280 and 443 to Asparag…

    Promoter

    T7

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pBOB-C-Flag-GK5-toku (1-174)

    Plasmid

    #134261

    Purpose

    Lentivector encoding Flag-tagged toku form of GK5 (amino acids 1-174)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk5 amino acids 1-174

    Promoter

    CMV

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pMBP-parallel1-GK5_v2 (D30N+D280N)

    Plasmid

    #134292

    Purpose

    Expresses GK5 (isoform 2)-D30N/D280N mutant in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed Aspartic Acids 30 and 280 to Asparagine

    Promoter

    T7

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pBOB-C-Flag-GK5 (416-534)

    Plasmid

    #134267

    Purpose

    Lentivector encoding Flag-tagged GK5 truncation (amino acids 416-534)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk5 amino acids 396-534

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pBOB-C-Flag-GK5 (396-534)

    Plasmid

    #134266

    Purpose

    Lentivector encoding Flag-tagged GK5 truncation (amino acids 396-534)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk5 amino acids 396-534

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pBOB-C-Flag-GK5 (287-534)

    Plasmid

    #134264

    Purpose

    Lentivector encoding Flag-tagged GK5 truncation (amino acids 287-534)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk5 amino acids 287-534

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pBOB-C-Flag-GK5 (25-287)

    Plasmid

    #134263

    Purpose

    Lentivector encoding Flag-tagged GK5 truncation (amino acids 25-287)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk5 amino acids 25-287

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge