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Plasmid#113619PurposeReporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFPDepositorInsert4-μHOM variant of EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-aC-KHC-BZ
Plasmid#160427PurposeExpression of c-luciferase from sp. Kornikeria hastingsi carriebowae (Belize)DepositorInsertluciferase
UseLuciferaseTags6x Histidine tag, Yeast alpha secretion factor, c…ExpressionYeastMutationPromoterAOXAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABE HSF Flag Ac
Plasmid#1949DepositorInsertHeat Shock Factor 1 (HSF1 Human)
UseRetroviralTagsflagExpressionMammalianMutationAcidic mutations (D416, E493, E496 A)PromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-aC-VTS-US
Plasmid#160425PurposeExpression of c-luciferase from sp. Vargula tsujii (USA)DepositorInsertluciferase
UseLuciferaseTags6x Histidine tag, Yeast alpha secretion factor, c…ExpressionYeastMutationSee depositor comments belowPromoterAOXAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-aC-MSVU-BZ
Plasmid#160426PurposeExpression of c-luciferase from sp. Maristella sp. SVU (Belize)DepositorInsertluciferase
UseLuciferaseTags6x Histidine tag, Yeast alpha secretion factor, c…ExpressionYeastMutationSee depositor comments belowPromoterAOXAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
eGFP Y93H AC editing reporter
Plasmid#198883PurposeExpress eGFP fluorescence after Y93H correction and mCherry as transfection controlDepositorInsertenhanced green fluorescent protein
UseLentiviralTagsExpressionMammalianMutationY93H, Gly92(GGT-to-GGA)PromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
env8 AC rep J1/13
Plasmid#99830PurposeGFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.DepositorInsertenv8 AC rep J1/13
UseTagsGFPuvExpressionBacterialMutationJ1/13 converted to repeating AC'sPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ac 1.8kb red opsin GFP.
Plasmid#72915Purposered opsin promoter from Carolina anole lizard (Anolis carolinensis) gDNA (nucleotides21,797 to 187, corresponding to chr2:88663108–88664997 in anoCar2.0) driving GFPDepositorInsertred opsin promoter
UseTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ac 1.8kb red opsin DsRed
Plasmid#72914Purposered opsin promoter from Carolina anole lizard (Anolis carolinensis) gDNA (nucleotides21,797 to 187, corresponding to chr2:88663108–88664997 in anoCar2.0) driving DsRedDepositorInsertred opsin promoter
UseTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Antibody#184201-rAbPurposeAnti-LRP4 (extracellular) (Mouse) recombinant mouse monoclonal antibodyDepositorRecommended ApplicationsImmunocytochemistry, Immunohistochemistry, and Western BlotReactivityMouse and RatSource SpeciesMouseIsotypeIgG2aTrial SizeAvailable to purchaseAvailable sinceAug. 11, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits
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Antibody#184174-rAbPurposeAnti-Pan-Neurofascin (extracellular) (Rat) recombinant mouse monoclonal antibodyDepositorRecommended ApplicationsImmunohistochemistry and Western BlotReactivityHuman and RatSource SpeciesMouseIsotypeIgG2aTrial SizeAvailable to purchaseAvailable sinceJune 13, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits
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JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)
Plasmid#156414Purposekdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4B-G12V (KRAS Human)
UseZebrafish transgenesisTagsEGFPExpressionMutationmutant KRAS4B-G12VPromoterkdrlAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 771 (pTol2-kdrl:EYFP-hsKRAS4B-WT-ac/Y)
Plasmid#156413Purposekdrl promoter driving EYFP fused to human KRAS4B with an alpha-crystallin zsYellow reporter flanked by Tol2 sitesDepositorInserthuman wild type KRAS4B-WT (KRAS Human)
UseZebrafish transgenesisTagsEYFPExpressionMutationPromoterkdrlAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)
Plasmid#156415Purposekdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4A-G12D (KRAS Human)
UseZebrafish transgenesisTagsmTagBFP2ExpressionMutationmutant KRAS4A-G12DPromoterkdrlAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 859 (pTol2-kdrl:V5-mScarlet-I-hsKRAS4A-G12V-ac/mC)
Plasmid#156416Purposekdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A-G12V with an alpha-crystallin mCerulean reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4A-G12V (KRAS Human)
UseZebrafish transgenesisTagsmScarlet-IExpressionMutationmutant KRAS4A-G12VPromoterkdrlAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 858 (pTol2-Kdrl-V5-mScarlet-KRAS4A-WT--AC-Cerulean)
Plasmid#180468Purposekdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A with an alpha-crystallin mCerulean reporter flanked by Tol2 sitesDepositorInsertKRAS4A (KRAS Human)
UseZebrafish transgenesisTagsV5-mScarlet-IExpressionMutationPromoterkdrlAvailable sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQexp
Plasmid#25820DepositorInsertpQexp
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
BL21 ΔrecBCD
Bacterial Strain#176581PurposeE. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.DepositorBacterial ResistanceNoneAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
BL21 Rosetta2 ΔrecBCD
Bacterial Strain#176583PurposeE. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.DepositorBacterial ResistanceChloramphenicolAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
BL21 ΔrecB
Bacterial Strain#176580PurposeE. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression.DepositorBacterial ResistanceNoneAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only