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Showing: 401 - 420 of 16657 results

  1. pX330-Flag-eSpCas9-H840A

    Plasmid
    #80454
    Purpose
    enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    ehSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80454-plasmid-map-sequence-id-151472

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  2. pET-FLAG-eSpCas9-plus

    Plasmid
    #126774
    Purpose
    Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    T7
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126774-plasmid-map-sequence-id-410603

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  4. mEmerald-Espin-like-ARD-C-18

    Plasmid
    #54088
    Purpose
    Localization: Actin Bundling, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Espin-like-ARD
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Ankyrin Repeat Domain
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. pBP-P_TALEsp1_PUPsp1-lacI_Ptac-lacO

    Plasmid
    #204079
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    TALEsp1-stabilized promoter driving lacI repressor; Ptac-lacO IPTG-inducible promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204079-plasmid-map-sequence-id-413894

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  11. pENTR-R2L3-cmER-IRESpuro-pA

    Plasmid
    #139539
    Purpose
    Provides mouse ER protein tag N-terminal to IRES-puro-pA cassette on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    ER
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    139539-plasmid-map-sequence-id-274701

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  12. pENTR-R2L3-cHA-IRESpuro-pA

    Plasmid
    #139537
    Purpose
    Provides HA protein tag N-terminal to IRES-puro-pA cassette on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    HA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139537-plasmid-map-sequence-id-274676

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  13. pENTR-R2L3-cGR-IRESpuro-pA

    Plasmid
    #139538
    Purpose
    Provides GR protein tag N-terminal to IRES-puro-pA cassette on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    GR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139538-plasmid-map-sequence-id-274620

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  16. mCherry-Espin-Like-ARD-C-18

    Plasmid
    #62743
    Purpose
    Localization: Actin Bundling, C1 Cloning Vector, Excitation:587, Emission:610
    Depositor
    Michael Davidson
    Insert
    EspinLikeARD
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 401 - 420 of 16657 results