We narrowed to 1,515 results for: Mbp

1. pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase

   Plasmid

   #196185

   Purpose

   Co-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-His

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

   Inserts

   GSK3B (GSK3B Human)
   λPPase
   

   Use

   Tags

   GST, HA, His6, and MBP

   Expression

   Bacterial

   Mutation

   changed Serine 9 to Alanine

   Promoter

   Tac

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-21a(+)/MBP-His-PINK1

   Plasmid

   #38005

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   PINK1 (PINK1 Human)
   

   Use

   Tags

   6xHIS and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pET His6 MBP TEV HDAC8_374

   Plasmid

   #122174

   Purpose

   Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag

   Depositor

   David Christianson

   Article

   Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22

   Insert

   His6 MBP TEV HDAC8_374 (HDAC8 Human)
   

   Use

   Tags

   hexahistidine tag and tobacco etch virus protease…

   Expression

   Bacterial

   Mutation

   Residues Met1–Pro7 and H375-V377 have been delete…

   Promoter

   T7

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-MBP-TEVpCS-ADGRB1 CTF-FLAG

   Plasmid

   #217681

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRB1 (ADGRB1 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-926

   Promoter

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax

   Plasmid

   #190153

   Purpose

   Expresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

   Depositor

   Brad Zuchero

   Article

   Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

   Insert

   Vamp2 (Vamp2 Mouse)
   

   Use

   AAV

   Tags

   P2AT2A-EGFP-caax

   Expression

   Mammalian

   Mutation

   Truncation that includes only AA #1-94

   Promoter

   MBP

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE-80L MBP-SspB Micro

   Plasmid

   #60410

   Purpose

   E. coli expression of SspB Micro

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   SspB micro
   

   Use

   Tags

   His-MBP-TEV

   Expression

   Bacterial

   Mutation

   R73Q, Y11K and A15E

   Promoter

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-MBP-TEVpCS-ADGRG6 CTF-FLAG

   Plasmid

   #217704

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGR6 (ADGRG6 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-840

   Promoter

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-MBP-TEVpCS-ADGRG6 CTFdeltaTA-FLAG

   Plasmid

   #217737

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGR6 (ADGRG6 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-846

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-MBP-TEVpCS-ADGRL1 CTFdeltaTA-FLAG

   Plasmid

   #217739

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRL1 (ADGRL1 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-844

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HA-MBP-TEVpCS-ADGRL2 CTFdeltaTA-FLAG

    Plasmid

    #217740

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRL2 (ADGRL2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-830

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-MBP-TEVpCS-ADGRL2 CTF-FLAG

    Plasmid

    #217707

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRL2 (ADGRL2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-824

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIneo-V5-SBP-MBP-HsStaufen1-T3_V

    Plasmid

    #147744

    Purpose

    Mammalian Expression of HsStaufen1-T3

    Depositor

    Elisa Izaurralde

    Insert

    HsStaufen1-T3 (STAU1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-His6-Sumo-CfaC-MBP

    Plasmid

    #126018

    Purpose

    Bacterial expression of CfaC-MBP fusion protein (with N-terminal His6-Sumo)

    Depositor

    Thomas Muir

    Insert

    His6-Sumo-CfaC-MBP
    

    Use

    Tags

    6xHis-SUMO and HA-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIneo-V5-SBP-MBP-HsStaufen1-T2_V

    Plasmid

    #147743

    Purpose

    Mammalian Expression of HsStaufen1-T2

    Depositor

    Elisa Izaurralde

    Insert

    HsStaufen1-T2 (STAU1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp

    Plasmid

    #183069

    Purpose

    Bacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c_4
    

    Use

    CRISPR

    Tags

    His10 and MBP

    Expression

    Bacterial

    Mutation

    Wild-type

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMJ106: pRS426-His-MBP-NlovFz2_Native

    Plasmid

    #205253

    Purpose

    S. cerevisiae expression vector for NlovFz2 native guide

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    NlovFz2
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMJ45: pRS426-His-MBP-SpuFz1_Native

    Plasmid

    #205252

    Purpose

    S. cerevisiae expression vector for SpuFz1 native guide

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMJ123: pRS426-His-MBP-SpuFz1_PSP1

    Plasmid

    #205256

    Purpose

    S. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMJ240: pRS426-His-MBP-MmeFz2_Native

    Plasmid

    #205255

    Purpose

    S. cerevisiae expression vector for MmeFz2 native guide

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    MmeFz2
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-MBP-TEVpCS-ADGRE3 CTFdeltaTA-FLAG

    Plasmid

    #217724

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRE3 (ADGRE3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-344

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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