We narrowed to 9,216 results for: Pol

1. pLV-CMV-EGFP-PolB-Hygro

   Plasmid

   #176056

   Purpose

   EGFP fused to the N-terminus of PolB & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PL452-hGH-polyA-PGK-neoR

   Plasmid

   #191681

   Purpose

   Donor plasmid backbone PGK-neoR

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Type

   Empty backbone

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA

   Plasmid

   #105648

   Purpose

   Expression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeks

   Depositor

   Sandra Rieger

   Insert

   Tol2-Isl2b:GFP:polyA (isl2b Zebrafish)
   

   Expression

   Mammalian

   Available Since

   Feb. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-TgKI-MCS-tdTomato-polyA

   Plasmid

   #174022

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   tdTomato
   

   Use

   Zebrafish knock-in tagging

   Available Since

   Nov. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. TDH3pro-rtTA-tetO7pro-T7pol-pRS413

   Plasmid

   #239293

   Purpose

   Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system

   Depositor

   Anita Hopper

   Article

   Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

   Inserts

   T7 polymerase with NLS and P278L
   reverse tetracycline transactivator (rtTA)
   

   Expression

   Yeast

   Mutation

   Contains M2-SE-G72P mutations, described in Addge…

   Promoter

   TDH3 and tetracycline-responsive element (TRE) an…

   Available Since

   June 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-45b-POLR2A-CTD-mCherry

   Plasmid

   #185011

   Purpose

   Bacterial Expression of 6xHis C-terminal domain of POLR2A mCherry Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   POLR2A (Polr2a Mouse)
   

   Expression

   Bacterial

   Available Since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pATetO 6XHis-Taq DNA Pol

   Plasmid

   #178782

   Purpose

   Inducible expression of Taq DNA polymerase in E. coli

   Depositor

   Andrew Ellington

   Article

   Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

   Insert

   Taq DNA Polymerase
   

   Expression

   Bacterial

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. LncRNA overexpression EF1α-BbsI-SV40polyA_puroR

   Plasmid

   #219826

   Purpose

   Overexpression vector for non-coding RNAs driven by EF1α promoter with puromycin resistance. Cloning using BbsI.

   Depositor

   Philipp Maass

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 29, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pHR_5xUAS-BFP-WPRE_PGK-HygR-bGHpolyA

   Plasmid

   #216665

   Purpose

   Expresses Gal4 inducible BFP, with hygromycin resistance

   Depositor

   Leonardo Morsut

   Inserts

   BFP
   HygromycinR
   

   Use

   Lentiviral

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

    Plasmid

    #208963

    Purpose

    Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    EcKlenow-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Poliovirus 1 PV1 isolate 09_05_19 3C protease

    Plasmid

    #228647

    Purpose

    Bacterial expression of the Polio 3C protease

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Polio virus 3C protease
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Available Since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. single polypeptide FPX biosensor for caspase-3

    Plasmid

    #60883

    Purpose

    FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for caspase-3
    

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-Ran-T24N-mRFP1-polyA

    Plasmid

    #104561

    Purpose

    Express Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran (Ran Mouse)
    

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    T24N

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-flag-hKDM4A(H188A)-polyA

    Plasmid

    #101052

    Purpose

    in vitro transcription (T7 promoter)

    Depositor

    Yi Zhang

    Article

    Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

    Insert

    KDM4A (KDM4A Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-Ran-Q69L-mRFP1-polyA

    Plasmid

    #104560

    Purpose

    Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran (Ran Mouse)
    

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    Q69L

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS aP2 promoter (5.4kb) polyA

    Plasmid

    #11424

    Depositor

    Ronald Kahn

    Insert

    aP2 promoter (Fabp4 Mouse)
    

    Expression

    Bacterial

    Available Since

    April 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1a-HA-hTet1CD-WPRE-PolyA

    Plasmid

    #39454

    Depositor

    Hongjun Song

    Article

    Guo et al Cell. 2011 Apr 29;145(3):423-34. Epub 20

    Insert

    HA-hTet1CD (TET1 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa1418–2136

    Promoter

    EF1a

    Available Since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

18. single polypeptide FPX biosensor for caspase-1

    Plasmid

    #60885

    Purpose

    FPX biosensor for caspase-1

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for caspase-1
    

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX

    Plasmid

    #163543

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    POLD2-APOBEC1-UdgX-nCas9-UdgX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFH2.30_Gag-MCP-dpol-T2A-eGFP

    Plasmid

    #205538

    Purpose

    Express HIV Gag-MCP-dpol-T2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    HIV Gag-MCP-dpol
    

    Expression

    Mammalian

    Mutation

    pol frameshift sequence recoded

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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