We narrowed to 1,383 results for: piggyBac

1. PB-Neo-TetO-Cdk12_Untagged

   Plasmid

   #127178

   Purpose

   Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
   

   Use

   Piggybac transposase vector

   Tags

   None

   Expression

   Mammalian

   Promoter

   TetO Promoter

   Available Since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB12A

   Plasmid

   #210035

   Purpose

   Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas12a
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   nucleoplasmin NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF1α-M2rtTA-T2A-Puro

   Plasmid

   #169175

   Purpose

   piggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174

   Depositor

   Igor Slukvin

   Article

   Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:

   Insert

   M2rtTA-T2A-Puro
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Mutation

   See Depositor Comments Below

   Promoter

   EF1a

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXL-BacII-CAG-Zeo-t2A-sHRPaNLG1

   Plasmid

   #79914

   Purpose

   piggyBac transposon vector, Zeocin-2A-sHRPbNLG1

   Depositor

   Joshua Sanes

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   Zeocin-t2A-splitHRP-aNLG1 (Nlgn1 Mouse)
   

   Use

   Piggybac

   Expression

   Mammalian

   Available Since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. EW375 (PB) (ZNF35/IKZF1tar-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF

   Plasmid

   #236177

   Purpose

   PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/IKZF1-driven BFP, and GFP-NZF dummy transcription factor

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Inserts

   EGFP-NZF
   H2B (H2BC11 Human)
   

   Tags

   mTagBFP2

   Expression

   Mammalian

   Promoter

   CMV and four ZNF35/IKZF1 sites

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. EW373 (PB) (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF

   Plasmid

   #236175

   Purpose

   PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF250-driven BFP, and GFP-NZF dummy transcription factor

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Inserts

   EGFP-NZF
   H2B (H2BC11 Human)
   

   Tags

   mTagBFP2

   Expression

   Mammalian

   Promoter

   CMV and four ZNF35/ZNF250 sites

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. EW374 (PB) (ZNF35/ZNF35tar-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF

   Plasmid

   #236176

   Purpose

   PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF35-driven BFP, and GFP-NZF dummy transcription factor

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Inserts

   EGFP-NZF
   H2B (H2BC11 Human)
   

   Tags

   mTagBFP2

   Expression

   Mammalian

   Promoter

   CMV and four ZNF35/ZNF35 sites

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB9v2

   Plasmid

   #210029

   Purpose

   Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas9
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS and nucleoplasmin NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB758

   Plasmid

   #210033

   Purpose

   Contains inducible Cas7-P2A-Cas5-T2A-Cas8, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas7-P2A-Cas5-T2A-Cas8
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB9

    Plasmid

    #210036

    Purpose

    Contains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFP

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas9
    puro-T2A-eGFP
    

    Use

    CRISPR

    Tags

    SV40 NLS and nucleoplasmin NLS

    Expression

    Mammalian

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-TAC-OK+9MS-KRAB-dCas9

    Plasmid

    #120357

    Purpose

    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    OK+9MS cassette
    

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Mutation

    Klf4 [1-483]

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-CAG-rtTA3G-IH

    Plasmid

    #214118

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    trTA3G
    

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. BII-tdT-inv-GFP

    Plasmid

    #133393

    Purpose

    Piggybac vector for bidirectional promoter reporter assay

    Depositor

    Blair Madison

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    No promoter

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-UBC-GCAMP6f-PGK-neo

    Plasmid

    #160049

    Purpose

    expression of GCaMP6f in a piggybac plasmid

    Depositor

    Xiaojun Lian

    Insert

    GCaMP6f
    

    Expression

    Mammalian

    Promoter

    Ubc

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-CAG-rtTAM2-IB

    Plasmid

    #214117

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    rtTAM2
    

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. BII-ChPtW-smURFP

    Plasmid

    #133399

    Purpose

    Piggybac vector for constitutive expression of smURFP-NLS

    Depositor

    Blair Madison

    Insert

    smURFP-NLS
    

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. BII-STAR-tdT

    Plasmid

    #133396

    Purpose

    Piggybac vector for ASCL2 reporter

    Depositor

    Blair Madison

    Insert

    tdTomato-NLS
    

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Stem Cell Specific ASCL2 response elements with L…

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. BII-ChPtW-iRFP702

    Plasmid

    #133398

    Purpose

    Piggybac vector for constitutive expression of iRFP702

    Depositor

    Blair Madison

    Insert

    IRFP-702
    

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. DBJS-p8.3

    Plasmid

    #246895

    Purpose

    Used for multi-site piggybac transposon insertion of osTIR1 ligase for overexpression

    Depositor

    David Bartel

    Insert

    osTIR1 cDNA transposon + Hygro resistance
    

    Expression

    Mammalian

    Available Since

    Oct. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSARGENT1

    Plasmid

    #216315

    Purpose

    Reporter cassette for SARGENT with piggyBac ITRs and 10x Capture Sequence

    Depositor

    Barak Cohen

    Insert

    tagBFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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