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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,531 results for: piggyBac

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Showing: 401 - 420 of 1531 results

  1. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid
    #84244
    Purpose
    Expresses GA-inducible VPR-Sa dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAI-tagBFP-Sa dCas9
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …
    Expression
    Mammalian
    Mutation
    D10A, N580A and Synonymous mutation in PYL1 to re…
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    84244-plasmid-map-sequence-id-160887

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  3. pB-CAG-PE2-NatMX

    Plasmid
    #158567
    Purpose
    Piggybac vector that allows for the generation of PE2-expressing stable cell lines
    Depositor
    Alejandro Chavez
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158567-plasmid-map-sequence-id-309882

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  5. PB{3xP3-dsRed2; β2Tubulin-mNeonGreen}

    Plasmid
    #236085
    Purpose
    PiggyBac plasmid for the insertion of a sperm-expressed green fluorescent protein in Aedes aegypti
    Depositor
    Leslie Vosshall
    Insert
    3xP3-dsRed2; β2Tubulin-mNeonGreen
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236085-plasmid-map-sequence-id-472661

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  6. DBJS-p8.72

    Plasmid
    #246901
    Purpose
    Used for multi-site piggybac transposon insertion of MCP-tagged CAPRIN1
    Depositor
    David Bartel
    Insert
    MCP::eGFP::CAPRIN1 + Hygro resistance (CAPRIN1 Human)
    Expression
    Mammalian
    Available since
    Nov. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    246901-plasmid-map-sequence-id-490585

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  7. DBJS-p8.3

    Plasmid
    #246895
    Purpose
    Used for multi-site piggybac transposon insertion of osTIR1 ligase for overexpression
    Depositor
    David Bartel
    Insert
    osTIR1 cDNA transposon + Hygro resistance
    Expression
    Mammalian
    Available since
    Oct. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    246895-plasmid-map-sequence-id-490558

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  8. pPB12A

    Plasmid
    #210035
    Purpose
    Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Publication
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas12a
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    nucleoplasmin NLS
    Expression
    Mammalian
    Promoter
    EF-1α and TRE3G
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210035-plasmid-map-sequence-id-413996

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  9. PB Neo* PB

    Plasmid
    #138413
    Purpose
    Minimal piggyBac ITR-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Mario Capecchi
    Insert
    Neo*
    Use
    Mouse Targeting; Piggybac
    Expression
    Bacterial and Mammalian
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138413-plasmid-map-sequence-id-316185

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  15. pPB-spCas9-pZeo

    Plasmid
    #214121
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    spCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    214121-plasmid-map-sequence-id-423619

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  17. pPB-PGK-mCherry.OVA(323-339)

    Plasmid
    #190005
    Purpose
    Piggybac construct for constitutive expression of mCherry and Chicken Ovalbumin OVA323-339 peptide fusion
    Depositor
    Siyuan Zhang
    Insert
    mCherry.OVA(323-339)
    Use
    Synthetic Biology; Piggybac
    Tags
    OVA(323-339)
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    190005-plasmid-map-sequence-id-382848

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  18. pPB9v2

    Plasmid
    #210029
    Purpose
    Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Publication
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Inserts
    Cas9
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    SV40 NLS and nucleoplasmin NLS
    Expression
    Mammalian
    Promoter
    EF-1α and TRE3G
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210029-plasmid-map-sequence-id-414001

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  20. pPB1163

    Plasmid
    #210032
    Purpose
    Contains Cas3 gRNA cloning site, inducible Cas11-P2A-Cas6-T2A-Cas3, rtTA-T2A-blast
    Depositor
    Michael Talkowski
    Publication
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas11-P2A-Cas6-T2A-Cas3
    rtta-T2A-blast
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    EF-1α and TRE3G
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210032-plasmid-map-sequence-id-413995

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Showing: 401 - 420 of 1531 results