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pscALPS- HIV2-ROD vpx

Plasmid

#115816

Purpose

Encodes codon optimized 3xFLAG-HIV2ROD Vpx (M15390) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HIV2ROD Vpx (M15390)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAd-Track Flag-SIRT1

Plasmid

#8438

Depositor

Pere Puigserver

Article

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

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C3 luc (T1 del)

Plasmid

#11358

Depositor

Donald McDonnell

Article

Fan et al Mol Endocrinol. 1996 Dec . 10(12):1605-1

Insert

C3 promoter (C3 Human)

Use

Luciferase

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)

Plasmid

#73404

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
PhCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG-SBP-STAG2 (WT)

Plasmid

#73963

Purpose

STAG2 cDNA (CCDS43990) wild type

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Tags

FLAG-SBP

Expression

Mammalian

Mutation

wild type

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
wt dynamin 1 pEGFP

Plasmid

#34680

Depositor

Sandra Schmid

Article

Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

Insert

dynamin 1ba (DNM1L Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only
pFastBac-hDicer

Plasmid

#89144

Purpose

Expression of hDicer in Sf9 Cells

Depositor

Jennifer Doudna

Article

MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

Insert

hDicer (DICER1 Human)

Tags

His6-tev

Expression

Insect

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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5HT6-YFP-Inp54p(D281A)

Plasmid

#96813

Purpose

phosphatase-dead control for 5HT6-YFP-Inp54p

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-Inp54p(D281A)

Tags

YFP

Expression

Mammalian

Mutation

D281A inactivates phosphatase activity

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot8

Plasmid

#37367

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsNot8 (CNOT8 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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NET31 mRFP-N2 (490)

Plasmid

#61984

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al PLoS One. 2014 Nov 11;9(11):e111851. doi

Insert

NET31 (TMEM209 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pES005 (pTet-qacR wt/pQacA-deGFP)

Plasmid

#69034

Purpose

reporter with wild-type qacR on single plasmid

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Inserts

qacR (qacR Synthetic)
deGFP

Tags

His6 tag

Expression

Bacterial

Promoter

pQacA and pTet

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR221-NR1H3

Plasmid

#79514

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H3 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

LXR Alpha (NR1H3 Human)

Use

Multisite gateway entry clone

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pEGB 35S:YFP:Tnos (GB0209)

Plasmid

#68219

Purpose

Transcriptional unit for Yellow Flurescent Protein plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

YFP

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pOPS0379

Plasmid

#115510

Purpose

Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing sfGFP

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Rlv3841pNifH(pOGG082) sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pVEX-CC1

Plasmid

#74170

Purpose

protein expression of chicken DCTN1 p150Glued AA 217-548 (CC1) in bacteria

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

DCTN1 (DCTN1 Chicken)

Tags

None

Expression

Bacterial

Promoter

Inducible Tac promoter

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only
pCALNL-NaChBac

Plasmid

#125580

Purpose

Cre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

NaChBac

Use

Cre/Lox

Tags

FLAG

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA/Myc DeltaN TCF4

Plasmid

#16513

Depositor

Bert Vogelstein

Article

Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

Insert

TCF4 (TCF7L2 Human)

Expression

Mammalian

Mutation

Delta N: lacks aa 1-32

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pMXs-IP GFP-Atg14

Plasmid

#38264

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

autophagy related genes 14 (ATG14 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only
rs214-minor

Plasmid

#113380

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2149943 minor allele (T)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2149943 minor allele

Use

Luciferase

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPA1L

Plasmid

#19484

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA1L (HSPA1L Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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