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NHA-Ago4

Plasmid

#21537

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago4 (AGO4 Human)

Tags

NHA

Expression

Mammalian

Available Since

Aug. 6, 2009

Availability

Academic Institutions and Nonprofits only
Flag Snail 2A4E

Plasmid

#16224

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

Flag

Expression

Mammalian

Mutation

2A4E: S97A, S101A, S108E, S112E, S116E, S120E

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pAW-NbVHH05-mCherry

Plasmid

#171572

Purpose

Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cells

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-mCherry (UBE2J1 Human)

Tags

mCherry

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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LDB-14-p65

Plasmid

#158985

Purpose

Expresses LDB-14 fused with a C-terminal p65 transcriptional activation domain in mammalian cells

Depositor

Liangcai Gu

Article

Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

Insert

LDB-14-p65

Tags

V5 tag , 6xHis tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p35S-A3A-PBE

Plasmid

#119773

Purpose

Conversion of C to T in potato

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

APOBEC3A-nCas9-NLS-UGI-NLS

Tags

NLS (flanking UGI)

Expression

Plant

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

CaMv 2x35S

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSW610 - GR-LhG4_BD

Plasmid

#115992

Purpose

entry vector for GreenGate cloning method, CDS, with adaptors for B and D modules

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

GR-LhG4_BD

Use

Unspecified

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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shPBRM1#32-Tet-pLKO-puro

Plasmid

#107399

Purpose

Lentiviral expression of shRNA targeting PBRM

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

shRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1mLCAD_FLAG

Plasmid

#24484

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Long chain acyl-CoA dehydrogenase (Acadl Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only
pCDH-MND-OsTIR1(F74G)-P2A-EGFP-AID2-EF1a-RFP

Plasmid

#232800

Purpose

Helper plasmid to trigger auxin-inducible-degradation of targeted proteins.

Depositor

Chunliang Li

Article

Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

Insert

OsTIR1 F74G EGFP-AID

Use

Lentiviral

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-LMP2A-IRES-mCherry

Plasmid

#220353

Purpose

Expresses EBV latent gene LMP2A in mammalian cells

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

LMP2A (LMP-2A Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Expression

Mammalian

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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LRTi

Plasmid

#214072

Purpose

Luminescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

Firefly luciferase

Expression

Mammalian

Mutation

Linker sequence present between firefly luciferas…

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBad33_ISYmu1_reRNA

Plasmid

#205358

Purpose

Bacterial expression of ISYmu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISYmu1 TnpB

Expression

Bacterial

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO‐puro‐2A‐mdx4cv‐GFP

Plasmid

#187053

Purpose

fluorescent reporter for adenine base editing of mdx4cv point mutation

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET28C-tCorn

Plasmid

#124588

Purpose

Bacterial Expression of tRNA-Corn aptamer

Depositor

Samie Jaffrey

Article

Song et al Nat Chem Biol. 2017 Nov;13(11):1187-1194

Insert

tRNA-Corn

Expression

Bacterial

Promoter

lacI

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMVSP6-NR2F2-SV40-PURO

Plasmid

#138361

Purpose

Lentiviral plasmid contains human NR2F2 driven by CMVSP6 and puromycin resistance for selection

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

Human NR2F2, with puromycin driven by SV40 for selection

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p5E-CMVSP6

Plasmid

#138356

Purpose

Gateway compatible 5' entry clone contains Cytomegalovirus and SP6 dual promoter(CMVSSP6)

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

CMV-SP6 promoter

Use

Gateway compatible

Promoter

CMV-SP6

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX puro TRIM21 C54Y - GFP

Plasmid

#116942

Purpose

Expression of TRIM21 C54Y -GFP fusion

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

TRIM21 C54Y-GFP (TRIM21 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

C54Y

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAC1814_pCR8-gSMN2-DN2

Plasmid

#118647

Purpose

Transient transfection; Expressed SMN2-DN2 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-DN2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBMN-dGBP1-TagBFP

Plasmid

#80087

Purpose

MMLV retroviral vector for expression of dGBP1-TagBFP with t-hcRed as an infection/transfection marker

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

dGBP1-TagBFP

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPERretro-Sirt7 shRNA2

Plasmid

#53145

Purpose

shRNAi vector for Sirt7

Depositor

Katrin Chua

Article

Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

Insert

Sirt7

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

H1 RNA polymerase III

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only