We narrowed to 54,324 results for: tra

1. pRS2 (CK2alpha', CK2beta)

   Plasmid

   #27093

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Inserts

   CK2beta (CSNK2B Human)
   CK2alpha' (CSNK2A2 Human)
   

   Use

   Tetracycline-regulated expression

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   bidirectional tet-responsive promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGV13 (CK2alpha K68M, CK2beta)

   Plasmid

   #27094

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Inserts

   CK2beta (CSNK2B Human)
   CK2alpha K68M (CSNK2A1 Human)
   

   Use

   Tetracycline-regulated expression

   Tags

   HA on CK2alpha and Myc on CK2beta

   Expression

   Mammalian

   Mutation

   K68M on CK2alpha

   Promoter

   bidirectional tet-responsive promoter and bidirec…

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGV7 (CK2alpha' K69M, CK2beta)

   Plasmid

   #27095

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Inserts

   CK2beta (CSNK2B Human)
   CK2alpha' K69M (CSNK2A2 Human)
   

   Use

   Tetracycline-regulated expression

   Tags

   HA tag on CK2alpha' and Myc on CK2beta

   Expression

   Mammalian

   Mutation

   K69M on CK2alpha'

   Promoter

   bidirectional tet-responsive promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDCAF3-IS5

   Plasmid

   #65220

   Purpose

   Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.

   Depositor

   Jeffrey Barrick

   Article

   Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.

   Inserts

   ndmA
   ndmB
   ndmC
   ndmD
   gst9
   chlR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Changed start codon from gtg to atg and Changed s…

   Promoter

   BBa_J23100

   Available since

   Jan. 8, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCEP4-myc-PDGFRA-R914W_EGFP-PEST_reporter

   Plasmid

   #172596

   Purpose

   Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-R914W mutant signaling.

   Depositor

   Daniel Kiss

   Article

   Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

   Insert

   myc-PDGFRA-R914W (PDGFRA Synthetic, Human)
   

   Use

   Egfp-pest_reporter

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   PDGFRA-R914W

   Promoter

   CMV

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGB1alpha2_KanR_BastaR_Rb7_GFP

   Plasmid

   #186427

   Purpose

   optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)

   Depositor

   Lukas Fischer

   Article

   Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

   Inserts

   KanR
   BastaR
   Rb7
   eGFP
   

   Use

   Synthetic Biology; Binary vector for escherichia …

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   CaMV 35S and Pnos

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLViN-iRFP670-α-cateninA+ΔβH

   Plasmid

   #229707

   Purpose

   Lentiviral expression of iRFP670-alpha-cateninA+delta-beta-H in mammalian cells

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   amino acids 670-673, RAIM--> GSGS

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Nav1.5_1-2linker411-717_PPSYtoAASA1960-1996

   Plasmid

   #187732

   Purpose

   Express a chimera of the Nav1.5 linker between the first and second transmembrane domain with the motif surrounding PPSY; residues 1974-1977 PPSY mutated to AASA

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   Nav1.5 1,2linker residues 411-717 and Nav1.5 residues 1960-1996 PPSY mutated to AASA (SCN5A Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   deletion of residues 1-410 and 718-1959 and 1997-…

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-myc-PDGFRA-D842V_EGFP-PEST_reporter

   Plasmid

   #172598

   Purpose

   Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V mutant signaling.

   Depositor

   Daniel Kiss

   Article

   Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

   Insert

   myc-PDGFRA-D842V (PDGFRA Synthetic, Human)
   

   Use

   Egfp-pest_reporter

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   PDGFRA-D842V

   Promoter

   CMV

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-myc-PDGFRA-D842V+R914W_EGFP-PEST_reporter

    Plasmid

    #172599

    Purpose

    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V+R914W mutant signaling.

    Depositor

    Daniel Kiss

    Article

    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

    Insert

    myc-PDGFRA-D842V+R914W (PDGFRA Synthetic, Human)
    

    Use

    Egfp-pest_reporter

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    PDGFRA-D842V+R914W

    Promoter

    CMV

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-myc-PDGFRA-K627R+R914W_EGFP-PEST_reporter

    Plasmid

    #172600

    Purpose

    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R+R914W mutant signaling.

    Depositor

    Daniel Kiss

    Article

    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

    Insert

    myc-PDGFRA-K627R+R914W (PDGFRA Synthetic, Human)
    

    Use

    Egfp-pest_reporter

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    PDGFRA-K627R+R914W

    Promoter

    CMV

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-myc-PDGFRA-K627R_EGFP-PEST_reporter

    Plasmid

    #172597

    Purpose

    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R mutant signaling.

    Depositor

    Daniel Kiss

    Article

    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

    Insert

    myc-PDGFRA-K627R (PDGFRA Synthetic, Human)
    

    Use

    Egfp-pest_reporter

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    PDGFRA-K627R

    Promoter

    CMV

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT2iU6Apowriter2N(#355)

    Plasmid

    #184106

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transient transgenesis + in vitro trans…

    Tags

    internal tag: 5myc

    Expression

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    Zf-Ubi+SP6

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGP-AAV-syn-jGCaMP7s-WPRE (AAV PHP.eB)

    Viral Prep

    #104487-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-jGCaMP7s-WPRE (#104487). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven GCaMP7s calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Promoter

    Syn

    Tags

    None

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-S5E2-GCaMP6f (AAV PHP.eB)

    Viral Prep

    #135632-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV-S5E2-GCaMP6f (#135632). In addition to the viral particles, you will also receive purified pAAV-S5E2-GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Promoter

    Tags

    None

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

16. pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (AAV PHP.eB)

    Viral Prep

    #104491-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (#104491). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven, Cre-dependent, GCaMP7s calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Promoter

    Syn

    Tags

    None

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (AAV Retrograde)

    Viral Prep

    #125712-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (#125712). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE plasmid DNA. CaMKIIa-driven expression of the optimized mosquito OPN3 rhodopsin in-frame with mScarlet. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

    Promoter

    short version of αCamKinase II promoter (0.4kb)

    Tags

    None

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

18. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV PHP.eB)

    Viral Prep

    #104492-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Promoter

    Syn

    Tags

    None

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV5)

    Viral Prep

    #112677-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Promoter

    EF1a

    Tags

    mCherry (Cre-negative cells), EGFP (Cre-positive cells)

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

20. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV1)

    Viral Prep

    #112677-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Promoter

    EF1a

    Tags

    mCherry (Cre-negative cells), EGFP (Cre-positive cells)

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only