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pRFHUE-eGFP

Plasmid

#89469

Purpose

GFP labelling of filamentous fungi by Agrobacterium tumefaciens mediated transformation

Depositor

Luis González-Candelas

Article

Crespo-Sempere et al Int J Food Microbiol. 2011 Aug 2;148(2):

Insert

eGFP

Use

Binary plasmid for atmt of filamentous fungi

Promoter

A. nidulans gpdA

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-C-STAT1-mGFP-P2A-Puro

Plasmid

#199345

Purpose

express murine STAT1 protein fused with mGFP

Depositor

Massimo Mallardo

Article

Caiazza et al Int J Mol Sci. 2022 Nov 17;23(22):14232.

Insert

signal transducer and activator of transcription 1 (Stat1 Mouse)

Use

Lentiviral

Tags

mGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGWB436

Plasmid

#68701

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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PL452-CLSTN2-LA-p2a-EGFP-hGHpA-PGK-neoR-CLSTN2-RA

Plasmid

#191688

Purpose

For knocking p2a-EFGP into CLSTN2 locus

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

CLSTN2 homology arms and p2a-EGFP

Expression

Mammalian

Mutation

WT

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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Lifeact-mScarlet3-S2

Plasmid

#246873

Purpose

Expression of mScarelt3-S2 fused with Lifeact. This construct is used for imaging actin filaments.

Depositor

Pingyong Xu

Article

Ding et al Nat Methods. 2025 Nov 26. doi: 10.1038/s

Insert

mScarlet3-S2

Tags

Lifeact

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pgRNA-bacteria

Plasmid

#44251

Purpose

Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

guide RNA (bacteria)

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available Since

April 5, 2013

Availability

Academic Institutions and Nonprofits only

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hIRmut-GFP

Plasmid

#22397

Depositor

Joseph Bass

Article

Ramos et al Proc Natl Acad Sci U S A. 2007 Jun 19. 1

Insert

IRmut

Tags

GFP

Expression

Mammalian

Mutation

changed Leu 62 to Proline

Available Since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-L10A-Halo

Plasmid

#247300

Purpose

Mammalian expression of the large ribosomal submit protein L10A tagged with HaloTag; for single-molecule tracking of ribosomes.

Depositor

Jennifer Lippincott-Schwartz

Article

Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

Insert

pUBC-L10A-Halo

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pNH604-Padh-OsTIR1(F74G)

Plasmid

#247606

Purpose

Expression of OsTIR1

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

OsTIR1

Expression

Yeast

Mutation

F74G

Promoter

ADH1

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pNH605-Padh-OsTIR1(F74G)

Plasmid

#247605

Purpose

Expression of OsTIR1

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

OsTIR1

Expression

Yeast

Mutation

F74G

Promoter

ADH1

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pRS406-Padh-OsTIR1(F74G)

Plasmid

#247604

Purpose

Expression of OsTIR1

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

OsTIR1

Expression

Yeast

Mutation

F74G

Promoter

ADH1

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pRS405-Padh-OsTIR1(F74G)

Plasmid

#247603

Purpose

Expression of OsTIR1

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

OsTIR1

Expression

Yeast

Mutation

F74G

Promoter

ADH1

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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VN1852 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP4_Ddcm_WeaKRBS_TIR51_KanR

Plasmid

#235118

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses ASf1B and IP4.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP4

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN1316 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP4_Ddcm_eGFP-wcKanR

Plasmid

#235113

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP4.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP4

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-HA-Gna14

Plasmid

#246002

Purpose

Express HA N-terminal tagged mouse Galpha14 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gna14 (Gna14 Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pA-CBh.TP-(inactive)CNOT6-V5H6.MCh.Puro

Plasmid

#209943

Purpose

In mammalian cells expresses TP fused to an inactive CNOT6 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.

Depositor

Kevin Weeks

Article

Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38

Inserts

CCR4-NOT transcription complex subunit 6 (enzymatically inactive) (CNOT6 Human)
mCherry fluorescent protein fused to puromycin resistance marker

Tags

TP (MS2 coat protein) and V5 epitope with H6 tag

Expression

Mammalian

Promoter

CBh and CMV

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57KAN-10XALFA

Plasmid

#233106

Purpose

10X ALFA tag multimer

Depositor

Steve Stowers

Insert

ALFA tag

Use

Unspecified

Mutation

None

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-DEST-Hsp70-zCreI-mTagBFP2d-2xins (JDW 1002)

Plasmid

#229817

Purpose

A gateway compatible Tol2 destination vector containing the Hsp70 promoter driving Cre and TagBFP followed by 2 cHS4 insulator cores.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Tol2 destination vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pPMS-990

Plasmid

#232282

Purpose

Protein Expression

Depositor

Jeremy Nathans

Insert

Plxdc2 (Plxdc2 Mouse)

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-scFVtet2_bGHpA

Plasmid

#177352

Purpose

AAV expression of scFV-fused catalytic domain of TET2 for targeted DNA methylation editing

Depositor

Naoki Yamamoto

Article

Yamamoto et al Research Square [Preprint] 2021. doi: 10

Insert

Tet Methylcytosine Dioxygenase 2 (TET2 Human)

Use

AAV

Tags

myc and scFV

Expression

Mammalian

Mutation

Catalytic domains of human TET2 (1129–1936 and 14…

Promoter

CMV promoter

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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