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pLVX-NPM1wt-IRES-mCherry

Plasmid

#131824

Purpose

Lentiviral plasmid expressing NPM1 wildtype (human) in mammalian cells upon transduction

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Use

Lentiviral

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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tetO-FUW-eGFP-RHOA-Q63L

Plasmid

#73081

Purpose

Lentiviral transfer vector for tet-inducible expression of EGFP tagged human RHOA-Q63L

Depositor

Andrew Putnam

Article

Kong et al Sci Rep. 2013 Dec 11;3:3474. doi: 10.103

Insert

RHOA-Q63L (RHOA Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Q63L Constitutively active

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pE-SUMO CRYAA

Plasmid

#80756

Purpose

Expression of human alpha A-crystallin in E. coli

Depositor

Larry David

Article

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYAA (CRYAA Human)

Tags

6xHIS and Smt3 SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pE-SUMO CRYGD

Plasmid

#80755

Purpose

Expression of human gamma D-crystallin in E. coli

Depositor

Larry David

Article

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYGD (CRYGD Human)

Tags

6xHIS and Smt3 SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pDY118A

Plasmid

#182958

Purpose

pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.

Depositor

Shelley Copley

Article

Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

Inserts

Cas9
Gam, Beta, Exo
I-scel

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCIBN(deltaNLS)-pmGFP

Plasmid

#26867

Purpose

Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)

Tags

EGFP

Expression

Mammalian

Mutation

NLS sequences mutated

Promoter

CMV

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pLVX-NPM1c-IRES-mCherry

Plasmid

#131825

Purpose

Lentiviral plasmid expressing NPM1 wildtype (cytoplasmic mutant, human) in mammalian cells upon transduction

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Use

Lentiviral

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pmScarlet_H2A_C1

Plasmid

#85051

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

H2A (H2AC16 Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pmScarlet_alphaTubulin_C1

Plasmid

#85045

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Alpha-Tubulin (TUBA1B Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_LKB1

Plasmid

#108111

Purpose

Lentiviral expression plasmid of human LKB1 cDNA with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

LKB1 (STK11 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS promoter

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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PB_tre_dCas9_VP160

Plasmid

#126031

Purpose

PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

dCas9 fusion with VP160
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

EF1 alpha and TRE

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag

Plasmid

#216696

Purpose

Synchronize trafficking of TNFalpha from the ER (RUCH system)

Depositor

Franck Perez

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Inserts

FIREmate-KDEL
Tumor Necrosis Factor alpha (TNFa)

Tags

FIREtag, Streptavidin Binding Protein (SBP), and …

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHIV-EF1a-2A-ZsGreen

Plasmid

#231985

Purpose

Empty backbone for expression of GoI with Bi-cistronic expression ZsGreen

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

2A-ZsGreen

Use

Lentiviral

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pmScarlet-i_alphaTubulin_C1

Plasmid

#85047

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Alpha-Tubulin (TUBA1B Human)

Tags

mScarlet-i

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3/HA-FLAG-RBP2

Plasmid

#14800

Depositor

William Kaelin

Article

Klose et al Cell. 2007 Mar 9. 128(5):889-900.

Insert

Retinoblastoma Binding Protein 2 (KDM5A Human)

Tags

FLAG and HA

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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NSP4-EGFP

Plasmid

#210378

Purpose

Expresses SARS-CoV-2 NSP4 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

NSP4 (ORF1ab SARS-CoV-2)

Tags

EGFP

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-MYO1D

Plasmid

#125125

Purpose

Expresses human FLAG-MYO1D

Depositor

Bruce Beutler

Article

McAlpine et al Dis Model Mech. 2018 Sep 27;11(9). pii:

Insert

MYO1D (MYO1D Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-VprBP

Plasmid

#133586

Purpose

Expresses human VprBP wildtype in mammalian cells

Depositor

Yue Xiong

Article

Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

Insert

Vpr (HIV-1) binding protein (DCAF1 Human)

Tags

HA

Expression

Mammalian

Mutation

Wildtype human VprBP (Dcaf1)

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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CanlonicSF

Plasmid

#178342

Purpose

To explore lactate dynamics in intact systems

Depositor

Alejandro San Martín

Article

Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

Insert

CanlonicSF

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TetON-Puro-HIF1a 3809

Plasmid

#118705

Purpose

Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

shRNA against human HIF1a

Use

Lentiviral

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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