We narrowed to 5,115 results for: LucY

1. pmirGlo-RBM8A-3'UTR 240-546

   Plasmid

   #158108

   Purpose

   reporter 3'UTR hRBM8A (short)

   Depositor

   Emanuela Felley-Bosco

   Article

   Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

   Insert

   3'UTR of hRBM8A (RBM8A Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNOC_dCas9_sgRNA Td2

   Plasmid

   #176258

   Purpose

   pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   dead spCas9
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Mutation

   H840A and D10A mutations on spCas9 to inactivate …

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNOC_dfnCas12a_crRNA Td2

   Plasmid

   #176255

   Purpose

   pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Mutation

   D917A and E1006A mutations to inactivate the endo…

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pmirGlo-CALB2-3'UTR delta ARE&mir30

   Plasmid

   #74430

   Purpose

   Calb2 UTR with mutated AU binding sequence and mir-30 site 1 and 2

   Depositor

   Rolf Stahel, Emanuela Felley-Bosco

   Article

   Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

   Insert

   CALB2 3'UTR (CALB2 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. HNF4A_P2-198

   Plasmid

   #31065

   Depositor

   Gerhart Ryffel

   Article

   Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

   Insert

   HNF4A P2 promoter (HNF4A Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   -198 to -1 of P2 HNF4A promoter

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-EF1a-eFFly-mCherry

   Plasmid

   #104833

   Purpose

   3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detection

   Depositor

   Irmela Jeremias

   Article

   Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

   Inserts

   eFFly
   mCherry
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5/FRT/TO-GAlphaQ-RLuc8

   Plasmid

   #140982

   Purpose

   Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphaQ-RLuc8 (GNAQ Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA5/FRT/TO-GAlpha15-RLuc8

   Plasmid

   #140984

   Purpose

   Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlpha15-RLuc8 (GNA15 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. p28-2iDMD-puro

   Plasmid

   #88895

   Purpose

   28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.

   Depositor

   Michele Calos

   Article

   Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/

   Inserts

   Dystrophin (DMD Human)
   luciferase
   puromycin resistance enzyme
   mCherry
   

   Expression

   Mammalian

   Mutation

   codon optimized

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tet-O-FUW-ZEB2-P2A-mCherry

    Plasmid

    #203857

    Purpose

    Tet-inducible lentiviral plasmid expressing ZEB2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    ZEB2 (ZEB2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO-GAlphaGustducin-RLuc8

    Plasmid

    #140979

    Purpose

    Encodes a G alpha subunit (GNAT3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaGustducin-RLuc8 (GNAT3 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL3-TGFb1

    Plasmid

    #101762

    Purpose

    TGFb1 promoter reporter

    Depositor

    Yuh-Shan Jou

    Article

    Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

    Insert

    transforming growth factor beta 1 promoter (TGFB1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPGC1a_Proximal Promoter-FLuc2P

    Plasmid

    #154259

    Purpose

    Destabilized firefly luciferase reporter containing the Human PGC-1alpha Proximal Promoter from -500 to +52.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    PGC-1a Proximal Promoter (PPARGC1A Human)
    

    Expression

    Mammalian

    Promoter

    Human PGC-1alpha Proximal Promoter

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPGC1a_Distal Promoter-FLuc2P

    Plasmid

    #154260

    Purpose

    Destabilized firefly luciferase reporter containing the upstream Human PGC-1alpha Distal Promoter from -500 to +52.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    PGC-1a Distal Promoter (PPARGC1A Human)
    

    Expression

    Mammalian

    Promoter

    Human PGC-1alpha Distal Promoter

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAi14-luc-MC

    Plasmid

    #87113

    Purpose

    minicircle parental backbone plasmid including luc-pA knock-in donor and one cutting site for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    Luciferase-SV40pA
    

    Use

    CRISPR and Luciferase

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNOC_dfnCas12a-KRAB_crRNA NC

    Plasmid

    #176262

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    Kruppel associated box domain and luciferase

    Mutation

    D917A and E1006A mutations to inactivate the endo…

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid

    #176264

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    Kruppel associated box domain and luciferase

    Mutation

    H840A and D10A mutations on spCas9 to inactivate …

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pINSPECT_5'-xrRNA_NLuc_3'-HCV-UTR

    Plasmid

    #197265

    Purpose

    Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' HCV-UTR). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

    Type

    Empty backbone

    Use

    CRISPR, Luciferase, Synthetic Biology, and Unspec…

    Expression

    Mammalian

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pINSPECT_5'-xrRNA_NLuc_3'-XAP1

    Plasmid

    #197264

    Purpose

    Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

    Type

    Empty backbone

    Use

    CRISPR, Luciferase, Synthetic Biology, and Unspec…

    Expression

    Mammalian

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-p57

    Plasmid

    #101760

    Purpose

    p57 promoter reporter

    Depositor

    Yuh-Shan Jou

    Article

    Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

    Insert

    cyclin dependent kinase inhibitor 1C promoter (CDKN1C Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge